Cargando…

High Sensitivity Detection and Quantitation of DNA Copy Number and Single Nucleotide Variants with Single Color Droplet Digital PCR

[Image: see text] In this study, we present a highly customizable method for quantifying copy number and point mutations utilizing a single-color, droplet digital PCR platform. Droplet digital polymerase chain reaction (ddPCR) is rapidly replacing real-time quantitative PCR (qRT-PCR) as an efficient...

Descripción completa

Detalles Bibliográficos
Autores principales:	Miotke, Laura, Lau, Billy T., Rumma, Rowza T., Ji, Hanlee P.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2014
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3982983/ https://www.ncbi.nlm.nih.gov/pubmed/24483992 http://dx.doi.org/10.1021/ac403843j

Ejemplares similares

Correction to High Sensitivity Detection and Quantitation of DNA Copy Number and Single Nucleotide Variants with Single Color Droplet Digital PCR
por: Miotke, Laura, et al.
Publicado: (2014)

Correction to High Sensitivity Detection and Quantitation of DNA Copy Number and Single Nucleotide Variants with Single Color Droplet Digital PCR
por: Miotke, Laura, et al.
Publicado: (2015)

Quantification of Plasmid Copy Number with Single Colour Droplet Digital PCR
por: Plotka, Magdalena, et al.
Publicado: (2017)

Quantitative mitochondrial DNA copy number determination using droplet digital PCR with single-cell resolution
por: O'Hara, Ryan, et al.
Publicado: (2019)

Single-Color Digital PCR Provides High-Performance Detection of Cancer Mutations from Circulating DNA
por: Wood-Bouwens, Christina, et al.
Publicado: (2017)

High-Throughput Droplet Digital PCR System for Absolute Quantitation of DNA Copy Number
por: Hindson, Benjamin J., et al.
Publicado: (2011)

DNA copy number concentration measured by digital and droplet digital quantitative PCR using certified reference materials
por: Corbisier, Philippe, et al.
Publicado: (2015)

Quantitative Analysis of Single Nucleotide Polymorphisms within Copy Number Variation
por: Lee, Soohyun, et al.
Publicado: (2008)

Copy number variability of expression plasmids determined by cell sorting and Droplet Digital PCR
por: Jahn, Michael, et al.
Publicado: (2016)

Quantification of rare somatic single nucleotide variants by droplet digital PCR using SuperSelective primers
por: Pablo-Fontecha, Verónica, et al.
Publicado: (2023)

Clinically Applicable Assessment of Tisagenlecleucel CAR T Cell Treatment by Digital Droplet PCR for Copy Number Variant Assessment
por: Gkazi, Soragia Athina, et al.
Publicado: (2022)

Detection of MET Gene Copy Number in Cancer Samples Using the Droplet Digital PCR Method
por: Zhang, Yanni, et al.
Publicado: (2016)

Copy Number Variation and Rearrangements Assessment in Cancer: Comparison of Droplet Digital PCR with the Current Approaches
por: Cusenza, Vincenza Ylenia, et al.
Publicado: (2021)

Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation
por: Whale, Alexandra S., et al.
Publicado: (2012)

Comparison of single copy gene-based duplex quantitative PCR and digital droplet PCR for monitoring of expansion of CD19-directed CAR T cells in treated patients
por: Schubert, Maria-Luisa, et al.
Publicado: (2022)

Measurement of copy number variation in single cancer cells using rapid-emulsification digital droplet MDA
por: Kim, Samuel C., et al.
Publicado: (2017)

Bayesian estimation of genomic copy number with single nucleotide polymorphism genotyping arrays
por: Guo, Beibei, et al.
Publicado: (2010)

Application of Droplet Digital PCR for Estimating Vector Copy Number States in Stem Cell Gene Therapy
por: Lin, Huan-Ting, et al.
Publicado: (2016)

Using droplet digital PCR to analyze MYCN and ALK copy number in plasma from patients with neuroblastoma
por: Lodrini, Marco, et al.
Publicado: (2017)

Application of droplet digital PCR for the detection of vector copy number in clinical CAR/TCR T cell products
por: Lu, Alex, et al.
Publicado: (2020)

Accurate bulk quantitation of droplet digital PCR
por: Sun, Chen, et al.
Publicado: (2021)

Killer-cell Immunoglobulin-like Receptor gene linkage and copy number variation analysis by droplet digital PCR
por: Roberts, Chrissy h, et al.
Publicado: (2014)

Droplet digital PCR shows the D-Loop to be an error prone locus for mitochondrial DNA copy number determination
por: Li, Brian, et al.
Publicado: (2018)

Detection of clinically relevant copy number alterations in oral cancer progression using multiplexed droplet digital PCR
por: Hughesman, Curtis B., et al.
Publicado: (2017)

Rapid screening of copy number variations in STRC by droplet digital PCR in patients with mild-to-moderate hearing loss
por: Ito, Taku, et al.
Publicado: (2019)

Detection and quantification of integrated vector copy number by multiplex droplet digital PCR in dual-transduced CAR T cells
por: Wang, Wei, et al.
Publicado: (2023)

Relationship between clinical features and droplet digital PCR copy number in non-HIV patients with pneumocystis pneumonia
por: Bian, Wenjie, et al.
Publicado: (2023)

Quantitative telomerase enzyme activity determination using droplet digital PCR with single cell resolution
por: Ludlow, Andrew T., et al.
Publicado: (2014)

VCS: Tool for Visualizing Copy Number Variation and Single Nucleotide Polymorphism
por: Kim, HyoYoung, et al.
Publicado: (2014)

Differences in AMY1 Gene Copy Numbers Derived from Blood, Buccal Cells and Saliva Using Quantitative and Droplet Digital PCR Methods: Flagging the Pitfall
por: Ooi, Delicia Shu Qin, et al.
Publicado: (2017)

Accuracy of analysis of cfDNA for detection of single nucleotide variants and copy number variants in breast cancer
por: Yang, Xin, et al.
Publicado: (2019)

Simultaneous Ultra-Sensitive Detection of Structural and Single Nucleotide Variants Using Multiplex Droplet Digital PCR in Liquid Biopsies from Children with Medulloblastoma
por: Arthur, Cecilia, et al.
Publicado: (2023)

Single and multi-laboratory validation of a droplet digital PCR method
por: Gatto, Francesco, et al.
Publicado: (2022)

A Robust Protocol for Using Multiplexed Droplet Digital PCR to Quantify Somatic Copy Number Alterations in Clinical Tissue Specimens
por: Hughesman, Curtis B., et al.
Publicado: (2016)

Comparative analysis of HER2 copy number between plasma and tissue samples in gastric cancer using droplet digital PCR
por: Kim, Boram, et al.
Publicado: (2020)

Development and Evaluation of a Droplet Digital PCR Assay for 8p23 β-Defensin Cluster Copy Number Determination
por: Wen, Tingting, et al.
Publicado: (2021)

Array Comparative Genomic Hybridisation and Droplet Digital PCR Uncover Recurrent Copy Number Variation of the TTN Segmental Duplication Region
por: Sagath, Lydia, et al.
Publicado: (2022)

Comparative analyses of seven algorithms for copy number variant identification from single nucleotide polymorphism arrays
por: Dellinger, Andrew E., et al.
Publicado: (2010)

The Contribution of Copy Number Variants and Single Nucleotide Polymorphisms to the Additive Genetic Variance of Carcass Traits in Cattle
por: Rafter, Pierce, et al.
Publicado: (2021)

Quantitative Analysis of Pork and Chicken Products by Droplet Digital PCR
por: Cai, Yicun, et al.
Publicado: (2014)

Cannot write session to /tmp/vufind_sessions/sess_4objs6s1m7il8m6ajfcollhfi4