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Kdm3a lysine demethylase is an Hsp90 client required for cytoskeletal rearrangements during spermatogenesis

The lysine demethylase Kdm3a (Jhdm2a, Jmjd1a) is required for male fertility, sex determination, and metabolic homeostasis through its nuclear role in chromatin remodeling. Many histone-modifying enzymes have additional nonhistone substrates, as well as nonenzymatic functions, contributing to the fu...

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Autores principales: Kasioulis, Ioannis, Syred, Heather M., Tate, Peri, Finch, Andrew, Shaw, Joseph, Seawright, Anne, Fuszard, Matt, Botting, Catherine H., Shirran, Sally, Adams, Ian R., Jackson, Ian J., van Heyningen, Veronica, Yeyati, Patricia L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3982988/
https://www.ncbi.nlm.nih.gov/pubmed/24554764
http://dx.doi.org/10.1091/mbc.E13-08-0471
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author Kasioulis, Ioannis
Syred, Heather M.
Tate, Peri
Finch, Andrew
Shaw, Joseph
Seawright, Anne
Fuszard, Matt
Botting, Catherine H.
Shirran, Sally
Adams, Ian R.
Jackson, Ian J.
van Heyningen, Veronica
Yeyati, Patricia L.
author_facet Kasioulis, Ioannis
Syred, Heather M.
Tate, Peri
Finch, Andrew
Shaw, Joseph
Seawright, Anne
Fuszard, Matt
Botting, Catherine H.
Shirran, Sally
Adams, Ian R.
Jackson, Ian J.
van Heyningen, Veronica
Yeyati, Patricia L.
author_sort Kasioulis, Ioannis
collection PubMed
description The lysine demethylase Kdm3a (Jhdm2a, Jmjd1a) is required for male fertility, sex determination, and metabolic homeostasis through its nuclear role in chromatin remodeling. Many histone-modifying enzymes have additional nonhistone substrates, as well as nonenzymatic functions, contributing to the full spectrum of events underlying their biological roles. We present two Kdm3a mouse models that exhibit cytoplasmic defects that may account in part for the globozoospermia phenotype reported previously. Electron microscopy revealed abnormal acrosome and manchette and the absence of implantation fossa at the caudal end of the nucleus in mice without Kdm3a demethylase activity, which affected cytoplasmic structures required to elongate the sperm head. We describe an enzymatically active new Kdm3a isoform and show that subcellular distribution, protein levels, and lysine demethylation activity of Kdm3a depended on Hsp90. We show that Kdm3a localizes to cytoplasmic structures of maturing spermatids affected in Kdm3a mutant mice, which in turn display altered fractionation of β-actin and γ-tubulin. Kdm3a is therefore a multifunctional Hsp90 client protein that participates directly in the regulation of cytoskeletal components.
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spelling pubmed-39829882014-06-30 Kdm3a lysine demethylase is an Hsp90 client required for cytoskeletal rearrangements during spermatogenesis Kasioulis, Ioannis Syred, Heather M. Tate, Peri Finch, Andrew Shaw, Joseph Seawright, Anne Fuszard, Matt Botting, Catherine H. Shirran, Sally Adams, Ian R. Jackson, Ian J. van Heyningen, Veronica Yeyati, Patricia L. Mol Biol Cell Articles The lysine demethylase Kdm3a (Jhdm2a, Jmjd1a) is required for male fertility, sex determination, and metabolic homeostasis through its nuclear role in chromatin remodeling. Many histone-modifying enzymes have additional nonhistone substrates, as well as nonenzymatic functions, contributing to the full spectrum of events underlying their biological roles. We present two Kdm3a mouse models that exhibit cytoplasmic defects that may account in part for the globozoospermia phenotype reported previously. Electron microscopy revealed abnormal acrosome and manchette and the absence of implantation fossa at the caudal end of the nucleus in mice without Kdm3a demethylase activity, which affected cytoplasmic structures required to elongate the sperm head. We describe an enzymatically active new Kdm3a isoform and show that subcellular distribution, protein levels, and lysine demethylation activity of Kdm3a depended on Hsp90. We show that Kdm3a localizes to cytoplasmic structures of maturing spermatids affected in Kdm3a mutant mice, which in turn display altered fractionation of β-actin and γ-tubulin. Kdm3a is therefore a multifunctional Hsp90 client protein that participates directly in the regulation of cytoskeletal components. The American Society for Cell Biology 2014-04-15 /pmc/articles/PMC3982988/ /pubmed/24554764 http://dx.doi.org/10.1091/mbc.E13-08-0471 Text en © 2014 Kasioulis et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Kasioulis, Ioannis
Syred, Heather M.
Tate, Peri
Finch, Andrew
Shaw, Joseph
Seawright, Anne
Fuszard, Matt
Botting, Catherine H.
Shirran, Sally
Adams, Ian R.
Jackson, Ian J.
van Heyningen, Veronica
Yeyati, Patricia L.
Kdm3a lysine demethylase is an Hsp90 client required for cytoskeletal rearrangements during spermatogenesis
title Kdm3a lysine demethylase is an Hsp90 client required for cytoskeletal rearrangements during spermatogenesis
title_full Kdm3a lysine demethylase is an Hsp90 client required for cytoskeletal rearrangements during spermatogenesis
title_fullStr Kdm3a lysine demethylase is an Hsp90 client required for cytoskeletal rearrangements during spermatogenesis
title_full_unstemmed Kdm3a lysine demethylase is an Hsp90 client required for cytoskeletal rearrangements during spermatogenesis
title_short Kdm3a lysine demethylase is an Hsp90 client required for cytoskeletal rearrangements during spermatogenesis
title_sort kdm3a lysine demethylase is an hsp90 client required for cytoskeletal rearrangements during spermatogenesis
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3982988/
https://www.ncbi.nlm.nih.gov/pubmed/24554764
http://dx.doi.org/10.1091/mbc.E13-08-0471
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