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Nucleolus-tethering system (NoTS) reveals that assembly of photobodies follows a self-organization model

Protein–protein interactions play essential roles in regulating many biological processes. At the cellular level, many proteins form nuclear foci known as nuclear bodies in which many components interact with each other. Photobodies are nuclear bodies containing proteins for light-signaling pathways...

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Autores principales: Liu, Yin, Liu, Qi, Yan, Qingqing, Shi, Leilei, Fang, Yuda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983000/
https://www.ncbi.nlm.nih.gov/pubmed/24554768
http://dx.doi.org/10.1091/mbc.E13-09-0527
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author Liu, Yin
Liu, Qi
Yan, Qingqing
Shi, Leilei
Fang, Yuda
author_facet Liu, Yin
Liu, Qi
Yan, Qingqing
Shi, Leilei
Fang, Yuda
author_sort Liu, Yin
collection PubMed
description Protein–protein interactions play essential roles in regulating many biological processes. At the cellular level, many proteins form nuclear foci known as nuclear bodies in which many components interact with each other. Photobodies are nuclear bodies containing proteins for light-signaling pathways in plants. What initiates the formation of photobodies is poorly understood. Here we develop a nucleolar marker protein nucleolin2 (Nuc2)–based method called the nucleolus-tethering system (NoTS) by artificially tethering a protein of interest to the nucleolus to analyze the initiation of photobodies. A candidate initiator is evaluated by visualizing whether a protein fused with Nuc2 forms body-like structures at the periphery of the nucleolus, and other components are recruited to the de novo–formed bodies. The interaction between two proteins can also be revealed through relocation and recruitment of interacting proteins to the nucleolus. Using the NoTS, we test the interactions among components in photobodies. In addition, we demonstrate that components of photobodies such as CONSTITUTIVELY PHOTOMORPHOGENIC 1, photoreceptors, and transcription factors tethered to the nucleolus have the capacity to form body-like structures at the periphery of the nucleolus, which contain other components of photobodies, suggesting a self-organization model for the biogenesis of photobodies.
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spelling pubmed-39830002014-06-30 Nucleolus-tethering system (NoTS) reveals that assembly of photobodies follows a self-organization model Liu, Yin Liu, Qi Yan, Qingqing Shi, Leilei Fang, Yuda Mol Biol Cell Articles Protein–protein interactions play essential roles in regulating many biological processes. At the cellular level, many proteins form nuclear foci known as nuclear bodies in which many components interact with each other. Photobodies are nuclear bodies containing proteins for light-signaling pathways in plants. What initiates the formation of photobodies is poorly understood. Here we develop a nucleolar marker protein nucleolin2 (Nuc2)–based method called the nucleolus-tethering system (NoTS) by artificially tethering a protein of interest to the nucleolus to analyze the initiation of photobodies. A candidate initiator is evaluated by visualizing whether a protein fused with Nuc2 forms body-like structures at the periphery of the nucleolus, and other components are recruited to the de novo–formed bodies. The interaction between two proteins can also be revealed through relocation and recruitment of interacting proteins to the nucleolus. Using the NoTS, we test the interactions among components in photobodies. In addition, we demonstrate that components of photobodies such as CONSTITUTIVELY PHOTOMORPHOGENIC 1, photoreceptors, and transcription factors tethered to the nucleolus have the capacity to form body-like structures at the periphery of the nucleolus, which contain other components of photobodies, suggesting a self-organization model for the biogenesis of photobodies. The American Society for Cell Biology 2014-04-15 /pmc/articles/PMC3983000/ /pubmed/24554768 http://dx.doi.org/10.1091/mbc.E13-09-0527 Text en © 2014 Liu et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Liu, Yin
Liu, Qi
Yan, Qingqing
Shi, Leilei
Fang, Yuda
Nucleolus-tethering system (NoTS) reveals that assembly of photobodies follows a self-organization model
title Nucleolus-tethering system (NoTS) reveals that assembly of photobodies follows a self-organization model
title_full Nucleolus-tethering system (NoTS) reveals that assembly of photobodies follows a self-organization model
title_fullStr Nucleolus-tethering system (NoTS) reveals that assembly of photobodies follows a self-organization model
title_full_unstemmed Nucleolus-tethering system (NoTS) reveals that assembly of photobodies follows a self-organization model
title_short Nucleolus-tethering system (NoTS) reveals that assembly of photobodies follows a self-organization model
title_sort nucleolus-tethering system (nots) reveals that assembly of photobodies follows a self-organization model
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983000/
https://www.ncbi.nlm.nih.gov/pubmed/24554768
http://dx.doi.org/10.1091/mbc.E13-09-0527
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