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Time-Insensitive Fluorescent Sensor for Human Serum Albumin and Its Unusual Red Shift
[Image: see text] The concentration of human serum albumin (HSA) indicates the health state of individuals and is routinely measured by UV spectroscopy with bromocresol. However, this method tends to overestimate HSA, and more critically, depends highly on the timing, in seconds, of the measurements...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American
Chemical
Society
2014
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983026/ https://www.ncbi.nlm.nih.gov/pubmed/24527887 http://dx.doi.org/10.1021/ac5001256 |
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author | Smith, Sara E. Williams, Jessica M. Ando, Shin Koide, Kazunori |
author_facet | Smith, Sara E. Williams, Jessica M. Ando, Shin Koide, Kazunori |
author_sort | Smith, Sara E. |
collection | PubMed |
description | [Image: see text] The concentration of human serum albumin (HSA) indicates the health state of individuals and is routinely measured by UV spectroscopy with bromocresol. However, this method tends to overestimate HSA, and more critically, depends highly on the timing, in seconds, of the measurements. Here, we report an analog of 2′,7′-dichlorofluorescein that can be used as a fluorescent sensor to quantify HSA in human sera. The accuracy of this new method proved superior to that of bromocresol when an international standard serum sample was analyzed. This method is more convenient than the bromocresol method because it allows for fluorescence measurements during a >15 min period. Colorimetric analysis was also performed to further investigate the effects of the binding of the sensor to HSA. These spectroscopic studies suggest that absorption and emission changes upon HSA binding may be due to the dehydration of the dye and/or stabilization of the tritylic cation species. |
format | Online Article Text |
id | pubmed-3983026 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | American
Chemical
Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-39830262015-02-14 Time-Insensitive Fluorescent Sensor for Human Serum Albumin and Its Unusual Red Shift Smith, Sara E. Williams, Jessica M. Ando, Shin Koide, Kazunori Anal Chem [Image: see text] The concentration of human serum albumin (HSA) indicates the health state of individuals and is routinely measured by UV spectroscopy with bromocresol. However, this method tends to overestimate HSA, and more critically, depends highly on the timing, in seconds, of the measurements. Here, we report an analog of 2′,7′-dichlorofluorescein that can be used as a fluorescent sensor to quantify HSA in human sera. The accuracy of this new method proved superior to that of bromocresol when an international standard serum sample was analyzed. This method is more convenient than the bromocresol method because it allows for fluorescence measurements during a >15 min period. Colorimetric analysis was also performed to further investigate the effects of the binding of the sensor to HSA. These spectroscopic studies suggest that absorption and emission changes upon HSA binding may be due to the dehydration of the dye and/or stabilization of the tritylic cation species. American Chemical Society 2014-02-14 2014-03-04 /pmc/articles/PMC3983026/ /pubmed/24527887 http://dx.doi.org/10.1021/ac5001256 Text en Copyright © 2014 American Chemical Society |
spellingShingle | Smith, Sara E. Williams, Jessica M. Ando, Shin Koide, Kazunori Time-Insensitive Fluorescent Sensor for Human Serum Albumin and Its Unusual Red Shift |
title | Time-Insensitive Fluorescent Sensor for Human Serum
Albumin and Its Unusual Red Shift |
title_full | Time-Insensitive Fluorescent Sensor for Human Serum
Albumin and Its Unusual Red Shift |
title_fullStr | Time-Insensitive Fluorescent Sensor for Human Serum
Albumin and Its Unusual Red Shift |
title_full_unstemmed | Time-Insensitive Fluorescent Sensor for Human Serum
Albumin and Its Unusual Red Shift |
title_short | Time-Insensitive Fluorescent Sensor for Human Serum
Albumin and Its Unusual Red Shift |
title_sort | time-insensitive fluorescent sensor for human serum
albumin and its unusual red shift |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983026/ https://www.ncbi.nlm.nih.gov/pubmed/24527887 http://dx.doi.org/10.1021/ac5001256 |
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