Estimation of the Relative Sensitivity of qPCR Analysis Using Pooled Samples

The high sensitivity of qPCR makes it a desirable diagnostic method in epidemiological surveillance programs. However, due to high costs, the use of pooling has been suggested. In this paper, an algorithm based on the Montecarlo method has been designed and implemented. The algorithm had been tested...

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Detalles Bibliográficos
Autores principales: Muniesa, Ana, Ferreira, Chelo, Fuertes, Héctor, Halaihel, Nabil, de Blas, Ignacio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983103/
https://www.ncbi.nlm.nih.gov/pubmed/24722485
http://dx.doi.org/10.1371/journal.pone.0093491
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author Muniesa, Ana
Ferreira, Chelo
Fuertes, Héctor
Halaihel, Nabil
de Blas, Ignacio
author_facet Muniesa, Ana
Ferreira, Chelo
Fuertes, Héctor
Halaihel, Nabil
de Blas, Ignacio
author_sort Muniesa, Ana
collection PubMed
description The high sensitivity of qPCR makes it a desirable diagnostic method in epidemiological surveillance programs. However, due to high costs, the use of pooling has been suggested. In this paper, an algorithm based on the Montecarlo method has been designed and implemented. The algorithm had been tested in many different situations, and finally it was validated with a real dataset. Moreover, based on the results obtained and depending on pooling conditions, a drastic decrease of sensitivity is observed.
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spelling pubmed-39831032014-04-15 Estimation of the Relative Sensitivity of qPCR Analysis Using Pooled Samples Muniesa, Ana Ferreira, Chelo Fuertes, Héctor Halaihel, Nabil de Blas, Ignacio PLoS One Research Article The high sensitivity of qPCR makes it a desirable diagnostic method in epidemiological surveillance programs. However, due to high costs, the use of pooling has been suggested. In this paper, an algorithm based on the Montecarlo method has been designed and implemented. The algorithm had been tested in many different situations, and finally it was validated with a real dataset. Moreover, based on the results obtained and depending on pooling conditions, a drastic decrease of sensitivity is observed. Public Library of Science 2014-04-10 /pmc/articles/PMC3983103/ /pubmed/24722485 http://dx.doi.org/10.1371/journal.pone.0093491 Text en © 2014 Muniesa et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Muniesa, Ana
Ferreira, Chelo
Fuertes, Héctor
Halaihel, Nabil
de Blas, Ignacio
Estimation of the Relative Sensitivity of qPCR Analysis Using Pooled Samples
title Estimation of the Relative Sensitivity of qPCR Analysis Using Pooled Samples
title_full Estimation of the Relative Sensitivity of qPCR Analysis Using Pooled Samples
title_fullStr Estimation of the Relative Sensitivity of qPCR Analysis Using Pooled Samples
title_full_unstemmed Estimation of the Relative Sensitivity of qPCR Analysis Using Pooled Samples
title_short Estimation of the Relative Sensitivity of qPCR Analysis Using Pooled Samples
title_sort estimation of the relative sensitivity of qpcr analysis using pooled samples
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983103/
https://www.ncbi.nlm.nih.gov/pubmed/24722485
http://dx.doi.org/10.1371/journal.pone.0093491
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