Cargando…
Markers of Epithelial to Mesenchymal Transition in Association with Survival in Head and Neck Squamous Cell Carcinoma (HNSCC)
BACKGROUND: Elucidating the molecular phenotype of cancers with high metastatic potential will facilitate the development of novel therapeutic approaches to the disease. Gene expression profiles link epithelial to mesenchymal transition (EMT) phenotype with high-risk HNSCC. We sought to determine th...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983114/ https://www.ncbi.nlm.nih.gov/pubmed/24722213 http://dx.doi.org/10.1371/journal.pone.0094273 |
_version_ | 1782311264204095488 |
---|---|
author | Pectasides, Eirini Rampias, Theodoros Sasaki, Clarence Perisanidis, Christos Kouloulias, Vassilis Burtness, Barbara Zaramboukas, Thomas Rimm, David Fountzilas, George Psyrri, Amanda |
author_facet | Pectasides, Eirini Rampias, Theodoros Sasaki, Clarence Perisanidis, Christos Kouloulias, Vassilis Burtness, Barbara Zaramboukas, Thomas Rimm, David Fountzilas, George Psyrri, Amanda |
author_sort | Pectasides, Eirini |
collection | PubMed |
description | BACKGROUND: Elucidating the molecular phenotype of cancers with high metastatic potential will facilitate the development of novel therapeutic approaches to the disease. Gene expression profiles link epithelial to mesenchymal transition (EMT) phenotype with high-risk HNSCC. We sought to determine the role of protein biomarkers of EMT in head and neck squamous carcinoma (HNSC) prognosis. METHODS: Protein expression analysis of EGFR, β-catenin and E-cadherin was performed on a cohort of 102 patients with HNSCC recruited between 1992 and 2005 using automated quantitative protein analysis (AQUA). We evaluated associations with clinicopathological parameters and prognosis. RESULTS: There were 67 patients with primary squamous cell carcinoma of the head and neck in this cohort who met inclusion criteria and for whom we had complete E-cadherin, beta-catenin and EGFR expression data. High E-cadherin expressers had longer 5-year progression-free survival (PFS) compared to those with low E-cadherin (59.7% versus 40.6%, p = 0.04) and overall survival (OS) (69.6% versus 44.3%, p = 0.05). Kaplan-Meier analysis showed that patients with low beta-catenin-expressing tumors trended toward worse 5-year PFS (p = 0.057). High EGFR expressers had inferior OS compared to low EGFR expressers (27.7% vs. 54%, p = 0.029). In the multivariable analysis context, E-cadherin remained an independent predictor of improved OS (HR = 0.204, 95% CI 0.043 to 0.972, p = 0.046) while EGFR trended towards significance for OS. CONCLUSIONS: The putative markers of EMT defined within a panel of HNSCC using AQUA are associated with tumors of poor prognosis. |
format | Online Article Text |
id | pubmed-3983114 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-39831142014-04-15 Markers of Epithelial to Mesenchymal Transition in Association with Survival in Head and Neck Squamous Cell Carcinoma (HNSCC) Pectasides, Eirini Rampias, Theodoros Sasaki, Clarence Perisanidis, Christos Kouloulias, Vassilis Burtness, Barbara Zaramboukas, Thomas Rimm, David Fountzilas, George Psyrri, Amanda PLoS One Research Article BACKGROUND: Elucidating the molecular phenotype of cancers with high metastatic potential will facilitate the development of novel therapeutic approaches to the disease. Gene expression profiles link epithelial to mesenchymal transition (EMT) phenotype with high-risk HNSCC. We sought to determine the role of protein biomarkers of EMT in head and neck squamous carcinoma (HNSC) prognosis. METHODS: Protein expression analysis of EGFR, β-catenin and E-cadherin was performed on a cohort of 102 patients with HNSCC recruited between 1992 and 2005 using automated quantitative protein analysis (AQUA). We evaluated associations with clinicopathological parameters and prognosis. RESULTS: There were 67 patients with primary squamous cell carcinoma of the head and neck in this cohort who met inclusion criteria and for whom we had complete E-cadherin, beta-catenin and EGFR expression data. High E-cadherin expressers had longer 5-year progression-free survival (PFS) compared to those with low E-cadherin (59.7% versus 40.6%, p = 0.04) and overall survival (OS) (69.6% versus 44.3%, p = 0.05). Kaplan-Meier analysis showed that patients with low beta-catenin-expressing tumors trended toward worse 5-year PFS (p = 0.057). High EGFR expressers had inferior OS compared to low EGFR expressers (27.7% vs. 54%, p = 0.029). In the multivariable analysis context, E-cadherin remained an independent predictor of improved OS (HR = 0.204, 95% CI 0.043 to 0.972, p = 0.046) while EGFR trended towards significance for OS. CONCLUSIONS: The putative markers of EMT defined within a panel of HNSCC using AQUA are associated with tumors of poor prognosis. Public Library of Science 2014-04-10 /pmc/articles/PMC3983114/ /pubmed/24722213 http://dx.doi.org/10.1371/journal.pone.0094273 Text en © 2014 Pectasides et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Pectasides, Eirini Rampias, Theodoros Sasaki, Clarence Perisanidis, Christos Kouloulias, Vassilis Burtness, Barbara Zaramboukas, Thomas Rimm, David Fountzilas, George Psyrri, Amanda Markers of Epithelial to Mesenchymal Transition in Association with Survival in Head and Neck Squamous Cell Carcinoma (HNSCC) |
title | Markers of Epithelial to Mesenchymal Transition in Association with Survival in Head and Neck Squamous Cell Carcinoma (HNSCC) |
title_full | Markers of Epithelial to Mesenchymal Transition in Association with Survival in Head and Neck Squamous Cell Carcinoma (HNSCC) |
title_fullStr | Markers of Epithelial to Mesenchymal Transition in Association with Survival in Head and Neck Squamous Cell Carcinoma (HNSCC) |
title_full_unstemmed | Markers of Epithelial to Mesenchymal Transition in Association with Survival in Head and Neck Squamous Cell Carcinoma (HNSCC) |
title_short | Markers of Epithelial to Mesenchymal Transition in Association with Survival in Head and Neck Squamous Cell Carcinoma (HNSCC) |
title_sort | markers of epithelial to mesenchymal transition in association with survival in head and neck squamous cell carcinoma (hnscc) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983114/ https://www.ncbi.nlm.nih.gov/pubmed/24722213 http://dx.doi.org/10.1371/journal.pone.0094273 |
work_keys_str_mv | AT pectasideseirini markersofepithelialtomesenchymaltransitioninassociationwithsurvivalinheadandnecksquamouscellcarcinomahnscc AT rampiastheodoros markersofepithelialtomesenchymaltransitioninassociationwithsurvivalinheadandnecksquamouscellcarcinomahnscc AT sasakiclarence markersofepithelialtomesenchymaltransitioninassociationwithsurvivalinheadandnecksquamouscellcarcinomahnscc AT perisanidischristos markersofepithelialtomesenchymaltransitioninassociationwithsurvivalinheadandnecksquamouscellcarcinomahnscc AT koulouliasvassilis markersofepithelialtomesenchymaltransitioninassociationwithsurvivalinheadandnecksquamouscellcarcinomahnscc AT burtnessbarbara markersofepithelialtomesenchymaltransitioninassociationwithsurvivalinheadandnecksquamouscellcarcinomahnscc AT zaramboukasthomas markersofepithelialtomesenchymaltransitioninassociationwithsurvivalinheadandnecksquamouscellcarcinomahnscc AT rimmdavid markersofepithelialtomesenchymaltransitioninassociationwithsurvivalinheadandnecksquamouscellcarcinomahnscc AT fountzilasgeorge markersofepithelialtomesenchymaltransitioninassociationwithsurvivalinheadandnecksquamouscellcarcinomahnscc AT psyrriamanda markersofepithelialtomesenchymaltransitioninassociationwithsurvivalinheadandnecksquamouscellcarcinomahnscc |