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Quantitative Detection of Epstein-Barr Virus DNA in Cerebrospinal Fluid and Blood Samples of Patients with Relapsing-Remitting Multiple Sclerosis
The presence of Epstein-Barr Virus (EBV) DNA in cerebrospinal fluid (CSF) and peripheral blood (PB) samples collected from 55 patients with clinical and radiologically-active relapsing-remitting MS (RRMS) and 51 subjects with other neurological diseases was determined using standardized commercially...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983161/ https://www.ncbi.nlm.nih.gov/pubmed/24722060 http://dx.doi.org/10.1371/journal.pone.0094497 |
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author | Cocuzza, Clementina E. Piazza, Fabrizio Musumeci, Rosario Oggioni, Davide Andreoni, Simona Gardinetti, Margherita Fusco, Letizia Frigo, Maura Banfi, Paola Rottoli, Maria R. Confalonieri, Paolo Rezzonico, Monica Ferrò, Maria T. Cavaletti, Guido |
author_facet | Cocuzza, Clementina E. Piazza, Fabrizio Musumeci, Rosario Oggioni, Davide Andreoni, Simona Gardinetti, Margherita Fusco, Letizia Frigo, Maura Banfi, Paola Rottoli, Maria R. Confalonieri, Paolo Rezzonico, Monica Ferrò, Maria T. Cavaletti, Guido |
author_sort | Cocuzza, Clementina E. |
collection | PubMed |
description | The presence of Epstein-Barr Virus (EBV) DNA in cerebrospinal fluid (CSF) and peripheral blood (PB) samples collected from 55 patients with clinical and radiologically-active relapsing-remitting MS (RRMS) and 51 subjects with other neurological diseases was determined using standardized commercially available kits for viral nucleic acid extraction and quantitative EBV DNA detection. Both cell-free and cell-associated CSF and PB fractions were analyzed, to distinguish latent from lytic EBV infection. EBV DNA was detected in 5.5% and 18.2% of cell-free and cell-associated CSF fractions of patients with RRMS as compared to 7.8% and 7.8% of controls; plasma and peripheral blood mononuclear cells (PBMC) positivity rates were 7.3% and 47.3% versus 5.8% and 31.4%, respectively. No significant difference in median EBV viral loads of positive samples was found between RRMS and control patients in all tested samples. Absence of statistically significant differences in EBV positivity rates between RRMS and control patients, despite the use of highly sensitive standardized methods, points to the lack of association between EBV and MS disease activity. |
format | Online Article Text |
id | pubmed-3983161 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-39831612014-04-15 Quantitative Detection of Epstein-Barr Virus DNA in Cerebrospinal Fluid and Blood Samples of Patients with Relapsing-Remitting Multiple Sclerosis Cocuzza, Clementina E. Piazza, Fabrizio Musumeci, Rosario Oggioni, Davide Andreoni, Simona Gardinetti, Margherita Fusco, Letizia Frigo, Maura Banfi, Paola Rottoli, Maria R. Confalonieri, Paolo Rezzonico, Monica Ferrò, Maria T. Cavaletti, Guido PLoS One Research Article The presence of Epstein-Barr Virus (EBV) DNA in cerebrospinal fluid (CSF) and peripheral blood (PB) samples collected from 55 patients with clinical and radiologically-active relapsing-remitting MS (RRMS) and 51 subjects with other neurological diseases was determined using standardized commercially available kits for viral nucleic acid extraction and quantitative EBV DNA detection. Both cell-free and cell-associated CSF and PB fractions were analyzed, to distinguish latent from lytic EBV infection. EBV DNA was detected in 5.5% and 18.2% of cell-free and cell-associated CSF fractions of patients with RRMS as compared to 7.8% and 7.8% of controls; plasma and peripheral blood mononuclear cells (PBMC) positivity rates were 7.3% and 47.3% versus 5.8% and 31.4%, respectively. No significant difference in median EBV viral loads of positive samples was found between RRMS and control patients in all tested samples. Absence of statistically significant differences in EBV positivity rates between RRMS and control patients, despite the use of highly sensitive standardized methods, points to the lack of association between EBV and MS disease activity. Public Library of Science 2014-04-10 /pmc/articles/PMC3983161/ /pubmed/24722060 http://dx.doi.org/10.1371/journal.pone.0094497 Text en © 2014 Cocuzza et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Cocuzza, Clementina E. Piazza, Fabrizio Musumeci, Rosario Oggioni, Davide Andreoni, Simona Gardinetti, Margherita Fusco, Letizia Frigo, Maura Banfi, Paola Rottoli, Maria R. Confalonieri, Paolo Rezzonico, Monica Ferrò, Maria T. Cavaletti, Guido Quantitative Detection of Epstein-Barr Virus DNA in Cerebrospinal Fluid and Blood Samples of Patients with Relapsing-Remitting Multiple Sclerosis |
title | Quantitative Detection of Epstein-Barr Virus DNA in Cerebrospinal Fluid and Blood Samples of Patients with Relapsing-Remitting Multiple Sclerosis |
title_full | Quantitative Detection of Epstein-Barr Virus DNA in Cerebrospinal Fluid and Blood Samples of Patients with Relapsing-Remitting Multiple Sclerosis |
title_fullStr | Quantitative Detection of Epstein-Barr Virus DNA in Cerebrospinal Fluid and Blood Samples of Patients with Relapsing-Remitting Multiple Sclerosis |
title_full_unstemmed | Quantitative Detection of Epstein-Barr Virus DNA in Cerebrospinal Fluid and Blood Samples of Patients with Relapsing-Remitting Multiple Sclerosis |
title_short | Quantitative Detection of Epstein-Barr Virus DNA in Cerebrospinal Fluid and Blood Samples of Patients with Relapsing-Remitting Multiple Sclerosis |
title_sort | quantitative detection of epstein-barr virus dna in cerebrospinal fluid and blood samples of patients with relapsing-remitting multiple sclerosis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983161/ https://www.ncbi.nlm.nih.gov/pubmed/24722060 http://dx.doi.org/10.1371/journal.pone.0094497 |
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