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Viscoelastic Parameters for Quantifying Liver Fibrosis: Three-Dimensional Multifrequency MR Elastography Study on Thin Liver Rat Slices

OBJECTIVE: To assess in a high-resolution model of thin liver rat slices which viscoelastic parameter at three-dimensional multifrequency MR elastography has the best diagnostic performance for quantifying liver fibrosis. MATERIALS AND METHODS: The study was approved by the ethics committee for anim...

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Autores principales: Ronot, Maxime, Lambert, Simon A., Wagner, Mathilde, Garteiser, Philippe, Doblas, Sabrina, Albuquerque, Miguel, Paradis, Valérie, Vilgrain, Valérie, Sinkus, Ralph, Van Beers, Bernard E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983230/
https://www.ncbi.nlm.nih.gov/pubmed/24722733
http://dx.doi.org/10.1371/journal.pone.0094679
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author Ronot, Maxime
Lambert, Simon A.
Wagner, Mathilde
Garteiser, Philippe
Doblas, Sabrina
Albuquerque, Miguel
Paradis, Valérie
Vilgrain, Valérie
Sinkus, Ralph
Van Beers, Bernard E.
author_facet Ronot, Maxime
Lambert, Simon A.
Wagner, Mathilde
Garteiser, Philippe
Doblas, Sabrina
Albuquerque, Miguel
Paradis, Valérie
Vilgrain, Valérie
Sinkus, Ralph
Van Beers, Bernard E.
author_sort Ronot, Maxime
collection PubMed
description OBJECTIVE: To assess in a high-resolution model of thin liver rat slices which viscoelastic parameter at three-dimensional multifrequency MR elastography has the best diagnostic performance for quantifying liver fibrosis. MATERIALS AND METHODS: The study was approved by the ethics committee for animal care of our institution. Eight normal rats and 42 rats with carbon tetrachloride induced liver fibrosis were used in the study. The rats were sacrificed, their livers were resected and three-dimensional MR elastography of 5±2 mm liver slices was performed at 7T with mechanical frequencies of 500, 600 and 700 Hz. The complex shear, storage and loss moduli, and the coefficient of the frequency power law were calculated. At histopathology, fibrosis and inflammation were assessed with METAVIR score, fibrosis was further quantified with morphometry. The diagnostic value of the viscoelastic parameters for assessing fibrosis severity was evaluated with simple and multiple linear regressions, receiver operating characteristic analysis and Obuchowski measures. RESULTS: At simple regression, the shear, storage and loss moduli were associated with the severity of fibrosis. At multiple regression, the storage modulus at 600 Hz was the only parameter associated with fibrosis severity (r = 0.86, p<0.0001). This parameter had an Obuchowski measure of 0.89+/−0.03. This measure was significantly larger than that of the loss modulus (0.78+/−0.04, p = 0.028), but not than that of the complex shear modulus (0.88+/−0.03, p = 0.84). CONCLUSION: Our high resolution, three-dimensional multifrequency MR elastography study of thin liver slices shows that the storage modulus is the viscoelastic parameter that has the best association with the severity of liver fibrosis. However, its diagnostic performance does not differ significantly from that of the complex shear modulus.
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spelling pubmed-39832302014-04-15 Viscoelastic Parameters for Quantifying Liver Fibrosis: Three-Dimensional Multifrequency MR Elastography Study on Thin Liver Rat Slices Ronot, Maxime Lambert, Simon A. Wagner, Mathilde Garteiser, Philippe Doblas, Sabrina Albuquerque, Miguel Paradis, Valérie Vilgrain, Valérie Sinkus, Ralph Van Beers, Bernard E. PLoS One Research Article OBJECTIVE: To assess in a high-resolution model of thin liver rat slices which viscoelastic parameter at three-dimensional multifrequency MR elastography has the best diagnostic performance for quantifying liver fibrosis. MATERIALS AND METHODS: The study was approved by the ethics committee for animal care of our institution. Eight normal rats and 42 rats with carbon tetrachloride induced liver fibrosis were used in the study. The rats were sacrificed, their livers were resected and three-dimensional MR elastography of 5±2 mm liver slices was performed at 7T with mechanical frequencies of 500, 600 and 700 Hz. The complex shear, storage and loss moduli, and the coefficient of the frequency power law were calculated. At histopathology, fibrosis and inflammation were assessed with METAVIR score, fibrosis was further quantified with morphometry. The diagnostic value of the viscoelastic parameters for assessing fibrosis severity was evaluated with simple and multiple linear regressions, receiver operating characteristic analysis and Obuchowski measures. RESULTS: At simple regression, the shear, storage and loss moduli were associated with the severity of fibrosis. At multiple regression, the storage modulus at 600 Hz was the only parameter associated with fibrosis severity (r = 0.86, p<0.0001). This parameter had an Obuchowski measure of 0.89+/−0.03. This measure was significantly larger than that of the loss modulus (0.78+/−0.04, p = 0.028), but not than that of the complex shear modulus (0.88+/−0.03, p = 0.84). CONCLUSION: Our high resolution, three-dimensional multifrequency MR elastography study of thin liver slices shows that the storage modulus is the viscoelastic parameter that has the best association with the severity of liver fibrosis. However, its diagnostic performance does not differ significantly from that of the complex shear modulus. Public Library of Science 2014-04-10 /pmc/articles/PMC3983230/ /pubmed/24722733 http://dx.doi.org/10.1371/journal.pone.0094679 Text en © 2014 Ronot et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ronot, Maxime
Lambert, Simon A.
Wagner, Mathilde
Garteiser, Philippe
Doblas, Sabrina
Albuquerque, Miguel
Paradis, Valérie
Vilgrain, Valérie
Sinkus, Ralph
Van Beers, Bernard E.
Viscoelastic Parameters for Quantifying Liver Fibrosis: Three-Dimensional Multifrequency MR Elastography Study on Thin Liver Rat Slices
title Viscoelastic Parameters for Quantifying Liver Fibrosis: Three-Dimensional Multifrequency MR Elastography Study on Thin Liver Rat Slices
title_full Viscoelastic Parameters for Quantifying Liver Fibrosis: Three-Dimensional Multifrequency MR Elastography Study on Thin Liver Rat Slices
title_fullStr Viscoelastic Parameters for Quantifying Liver Fibrosis: Three-Dimensional Multifrequency MR Elastography Study on Thin Liver Rat Slices
title_full_unstemmed Viscoelastic Parameters for Quantifying Liver Fibrosis: Three-Dimensional Multifrequency MR Elastography Study on Thin Liver Rat Slices
title_short Viscoelastic Parameters for Quantifying Liver Fibrosis: Three-Dimensional Multifrequency MR Elastography Study on Thin Liver Rat Slices
title_sort viscoelastic parameters for quantifying liver fibrosis: three-dimensional multifrequency mr elastography study on thin liver rat slices
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983230/
https://www.ncbi.nlm.nih.gov/pubmed/24722733
http://dx.doi.org/10.1371/journal.pone.0094679
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