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Probing Tertiary Structure of Proteins Using Single Trp Mutations with Circular Dichroism at Low Temperature
[Image: see text] Trp is the most spectroscopically informative aromatic amino acid of proteins. However, the near-UV circular dichroism (CD) spectrum of Trp is complicated because the intensity and sign of (1)L(a) and (1)L(b) bands vary independently. To resolve vibronic structure and gain site-spe...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Chemical
Society
2014
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983331/ https://www.ncbi.nlm.nih.gov/pubmed/24404774 http://dx.doi.org/10.1021/jp4120145 |