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Atomic Force Microscopy Probing of Receptor–Nanoparticle Interactions for Riboflavin Receptor Targeted Gold–Dendrimer Nanocomposites

[Image: see text] Riboflavin receptors are overexpressed in malignant cells from certain human breast and prostate cancers, and they constitute a group of potential surface markers important for cancer targeted delivery of therapeutic agents and imaging molecules. Here we report on the fabrication a...

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Autores principales: Witte, Amanda B., Leistra, Abigail N., Wong, Pamela T., Bharathi, Sophia, Refior, Kevin, Smith, Phillip, Kaso, Ola, Sinniah, Kumar, Choi, Seok Ki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983334/
https://www.ncbi.nlm.nih.gov/pubmed/24571134
http://dx.doi.org/10.1021/jp412053w
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author Witte, Amanda B.
Leistra, Abigail N.
Wong, Pamela T.
Bharathi, Sophia
Refior, Kevin
Smith, Phillip
Kaso, Ola
Sinniah, Kumar
Choi, Seok Ki
author_facet Witte, Amanda B.
Leistra, Abigail N.
Wong, Pamela T.
Bharathi, Sophia
Refior, Kevin
Smith, Phillip
Kaso, Ola
Sinniah, Kumar
Choi, Seok Ki
author_sort Witte, Amanda B.
collection PubMed
description [Image: see text] Riboflavin receptors are overexpressed in malignant cells from certain human breast and prostate cancers, and they constitute a group of potential surface markers important for cancer targeted delivery of therapeutic agents and imaging molecules. Here we report on the fabrication and atomic force microscopy (AFM) characterization of a core–shell nanocomposite consisting of a gold nanoparticle (AuNP) coated with riboflavin receptor-targeting poly(amido amine) dendrimer. We designed this nanocomposite for potential applications such as a cancer targeted imaging material based on its surface plasmon resonance properties conferred by AuNP. We employed AFM as a technique for probing the binding interaction between the nanocomposite and riboflavin binding protein (RfBP) in solution. AFM enabled precise measurement of the AuNP height distribution before (13.5 nm) and after chemisorption of riboflavin-conjugated dendrimer (AuNP–dendrimer; 20.5 nm). Binding of RfBP to the AuNP–dendrimer caused a height increase to 26.7 nm, which decreased to 22.8 nm when coincubated with riboflavin as a competitive ligand, supporting interaction of AuNP–dendrimer and its target protein. In summary, physical determination of size distribution by AFM imaging can serve as a quantitative approach to monitor and characterize the nanoscale interaction between a dendrimer-covered AuNP and target protein molecules in vitro.
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spelling pubmed-39833342015-02-26 Atomic Force Microscopy Probing of Receptor–Nanoparticle Interactions for Riboflavin Receptor Targeted Gold–Dendrimer Nanocomposites Witte, Amanda B. Leistra, Abigail N. Wong, Pamela T. Bharathi, Sophia Refior, Kevin Smith, Phillip Kaso, Ola Sinniah, Kumar Choi, Seok Ki J Phys Chem B [Image: see text] Riboflavin receptors are overexpressed in malignant cells from certain human breast and prostate cancers, and they constitute a group of potential surface markers important for cancer targeted delivery of therapeutic agents and imaging molecules. Here we report on the fabrication and atomic force microscopy (AFM) characterization of a core–shell nanocomposite consisting of a gold nanoparticle (AuNP) coated with riboflavin receptor-targeting poly(amido amine) dendrimer. We designed this nanocomposite for potential applications such as a cancer targeted imaging material based on its surface plasmon resonance properties conferred by AuNP. We employed AFM as a technique for probing the binding interaction between the nanocomposite and riboflavin binding protein (RfBP) in solution. AFM enabled precise measurement of the AuNP height distribution before (13.5 nm) and after chemisorption of riboflavin-conjugated dendrimer (AuNP–dendrimer; 20.5 nm). Binding of RfBP to the AuNP–dendrimer caused a height increase to 26.7 nm, which decreased to 22.8 nm when coincubated with riboflavin as a competitive ligand, supporting interaction of AuNP–dendrimer and its target protein. In summary, physical determination of size distribution by AFM imaging can serve as a quantitative approach to monitor and characterize the nanoscale interaction between a dendrimer-covered AuNP and target protein molecules in vitro. American Chemical Society 2014-02-26 2014-03-20 /pmc/articles/PMC3983334/ /pubmed/24571134 http://dx.doi.org/10.1021/jp412053w Text en Copyright © 2014 American Chemical Society
spellingShingle Witte, Amanda B.
Leistra, Abigail N.
Wong, Pamela T.
Bharathi, Sophia
Refior, Kevin
Smith, Phillip
Kaso, Ola
Sinniah, Kumar
Choi, Seok Ki
Atomic Force Microscopy Probing of Receptor–Nanoparticle Interactions for Riboflavin Receptor Targeted Gold–Dendrimer Nanocomposites
title Atomic Force Microscopy Probing of Receptor–Nanoparticle Interactions for Riboflavin Receptor Targeted Gold–Dendrimer Nanocomposites
title_full Atomic Force Microscopy Probing of Receptor–Nanoparticle Interactions for Riboflavin Receptor Targeted Gold–Dendrimer Nanocomposites
title_fullStr Atomic Force Microscopy Probing of Receptor–Nanoparticle Interactions for Riboflavin Receptor Targeted Gold–Dendrimer Nanocomposites
title_full_unstemmed Atomic Force Microscopy Probing of Receptor–Nanoparticle Interactions for Riboflavin Receptor Targeted Gold–Dendrimer Nanocomposites
title_short Atomic Force Microscopy Probing of Receptor–Nanoparticle Interactions for Riboflavin Receptor Targeted Gold–Dendrimer Nanocomposites
title_sort atomic force microscopy probing of receptor–nanoparticle interactions for riboflavin receptor targeted gold–dendrimer nanocomposites
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983334/
https://www.ncbi.nlm.nih.gov/pubmed/24571134
http://dx.doi.org/10.1021/jp412053w
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