Enzyme-free Passage of Human Pluripotent Stem Cells by Controlling Divalent Cations

Enzymes used for passaging human pluripotent stem cells (hPSCs) digest cell surface proteins, resulting in cell damage. Moreover, cell dissociation using divalent cation-free solutions causes apoptosis. Here we report that Mg(2+) and Ca(2+) control cell-fibronectin and cell-cell binding of hPSCs, re...

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Detalles Bibliográficos
Autores principales: Ohnuma, Kiyoshi, Fujiki, Ayaka, Yanagihara, Kana, Tachikawa, Saoko, Hayashi, Yohei, Ito, Yuzuru, Onuma, Yasuko, Chan, Techuan, Michiue, Tatsuo, Furue, Miho K., Asashima, Makoto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983606/
https://www.ncbi.nlm.nih.gov/pubmed/24721898
http://dx.doi.org/10.1038/srep04646

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983606/
https://www.ncbi.nlm.nih.gov/pubmed/24721898
http://dx.doi.org/10.1038/srep04646

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