Cargando…

Licochalcone A induces apoptosis in KB human oral cancer cells via a caspase-dependent FasL signaling pathway

Licochalcone A (Lico-A) is a natural phenol licorice compound with multiple bioactivities, including anti-inflammatory, anti-microbial, anti-fungal and osteogenesis-inducing properties. In the present study, we investigated the Lico-A-induced apoptotic effects and examined the associated apoptosis p...

Descripción completa

Detalles Bibliográficos
Autores principales: KIM, JAE-SUNG, PARK, MI-RA, LEE, SOOK-YOUNG, KIM, DO KYOUNG, MOON, SUNG-MIN, KIM, CHUN SUNG, CHO, SEUNG SIK, YOON, GOO, IM, HEE-JEONG, YOU, JAE-SEEK, OH, JI-SU, KIM, SU-GWAN
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983909/
https://www.ncbi.nlm.nih.gov/pubmed/24337492
http://dx.doi.org/10.3892/or.2013.2929
_version_ 1782311379977371648
author KIM, JAE-SUNG
PARK, MI-RA
LEE, SOOK-YOUNG
KIM, DO KYOUNG
MOON, SUNG-MIN
KIM, CHUN SUNG
CHO, SEUNG SIK
YOON, GOO
IM, HEE-JEONG
YOU, JAE-SEEK
OH, JI-SU
KIM, SU-GWAN
author_facet KIM, JAE-SUNG
PARK, MI-RA
LEE, SOOK-YOUNG
KIM, DO KYOUNG
MOON, SUNG-MIN
KIM, CHUN SUNG
CHO, SEUNG SIK
YOON, GOO
IM, HEE-JEONG
YOU, JAE-SEEK
OH, JI-SU
KIM, SU-GWAN
author_sort KIM, JAE-SUNG
collection PubMed
description Licochalcone A (Lico-A) is a natural phenol licorice compound with multiple bioactivities, including anti-inflammatory, anti-microbial, anti-fungal and osteogenesis-inducing properties. In the present study, we investigated the Lico-A-induced apoptotic effects and examined the associated apoptosis pathway in KB human oral cancer cells. Lico-A decreased the number of viable KB oral cancer cells. However, Lico-A did not have an effect on primary normal human oral keratinocytes. In addition, the IC(50) value of Lico-A was determined to be ~50 μM following dose-dependent stimulation. KB oral cancer cells stimulated with Lico-A for 24 h showed chromatin condensation by DAPI staining, genomic DNA fragmentation by agarose gel electrophoresis and a gradually increased apoptotic cell population by FACS analysis. These data suggest that Lico-A induces apoptosis in KB oral cancer cells. Additionally, Lico-A-induced apoptosis in KB oral cancer cells was mediated by the expression of factor associated suicide ligand (FasL) and activated caspase-8 and −3 and poly(ADP-ribose) polymerase (PARP). Furthermore, in the KB oral cancer cells co-stimulation with a caspase inhibitor (Z-VAD-fmk) and Lico-A significantly abolished the apoptotic phenomena. Our findings demonstrated that Lico-A-induced apoptosis in KB oral cancer cells involves the extrinsic apoptotic signaling pathway, which involves a caspase-dependent FasL-mediated death receptor pathway. Our data suggest that Lico-A be developed as a chemotherapeutic agent for the management of oral cancer.
format Online
Article
Text
id pubmed-3983909
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-39839092014-04-11 Licochalcone A induces apoptosis in KB human oral cancer cells via a caspase-dependent FasL signaling pathway KIM, JAE-SUNG PARK, MI-RA LEE, SOOK-YOUNG KIM, DO KYOUNG MOON, SUNG-MIN KIM, CHUN SUNG CHO, SEUNG SIK YOON, GOO IM, HEE-JEONG YOU, JAE-SEEK OH, JI-SU KIM, SU-GWAN Oncol Rep Articles Licochalcone A (Lico-A) is a natural phenol licorice compound with multiple bioactivities, including anti-inflammatory, anti-microbial, anti-fungal and osteogenesis-inducing properties. In the present study, we investigated the Lico-A-induced apoptotic effects and examined the associated apoptosis pathway in KB human oral cancer cells. Lico-A decreased the number of viable KB oral cancer cells. However, Lico-A did not have an effect on primary normal human oral keratinocytes. In addition, the IC(50) value of Lico-A was determined to be ~50 μM following dose-dependent stimulation. KB oral cancer cells stimulated with Lico-A for 24 h showed chromatin condensation by DAPI staining, genomic DNA fragmentation by agarose gel electrophoresis and a gradually increased apoptotic cell population by FACS analysis. These data suggest that Lico-A induces apoptosis in KB oral cancer cells. Additionally, Lico-A-induced apoptosis in KB oral cancer cells was mediated by the expression of factor associated suicide ligand (FasL) and activated caspase-8 and −3 and poly(ADP-ribose) polymerase (PARP). Furthermore, in the KB oral cancer cells co-stimulation with a caspase inhibitor (Z-VAD-fmk) and Lico-A significantly abolished the apoptotic phenomena. Our findings demonstrated that Lico-A-induced apoptosis in KB oral cancer cells involves the extrinsic apoptotic signaling pathway, which involves a caspase-dependent FasL-mediated death receptor pathway. Our data suggest that Lico-A be developed as a chemotherapeutic agent for the management of oral cancer. D.A. Spandidos 2014-02 2013-12-16 /pmc/articles/PMC3983909/ /pubmed/24337492 http://dx.doi.org/10.3892/or.2013.2929 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
KIM, JAE-SUNG
PARK, MI-RA
LEE, SOOK-YOUNG
KIM, DO KYOUNG
MOON, SUNG-MIN
KIM, CHUN SUNG
CHO, SEUNG SIK
YOON, GOO
IM, HEE-JEONG
YOU, JAE-SEEK
OH, JI-SU
KIM, SU-GWAN
Licochalcone A induces apoptosis in KB human oral cancer cells via a caspase-dependent FasL signaling pathway
title Licochalcone A induces apoptosis in KB human oral cancer cells via a caspase-dependent FasL signaling pathway
title_full Licochalcone A induces apoptosis in KB human oral cancer cells via a caspase-dependent FasL signaling pathway
title_fullStr Licochalcone A induces apoptosis in KB human oral cancer cells via a caspase-dependent FasL signaling pathway
title_full_unstemmed Licochalcone A induces apoptosis in KB human oral cancer cells via a caspase-dependent FasL signaling pathway
title_short Licochalcone A induces apoptosis in KB human oral cancer cells via a caspase-dependent FasL signaling pathway
title_sort licochalcone a induces apoptosis in kb human oral cancer cells via a caspase-dependent fasl signaling pathway
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983909/
https://www.ncbi.nlm.nih.gov/pubmed/24337492
http://dx.doi.org/10.3892/or.2013.2929
work_keys_str_mv AT kimjaesung licochalconeainducesapoptosisinkbhumanoralcancercellsviaacaspasedependentfaslsignalingpathway
AT parkmira licochalconeainducesapoptosisinkbhumanoralcancercellsviaacaspasedependentfaslsignalingpathway
AT leesookyoung licochalconeainducesapoptosisinkbhumanoralcancercellsviaacaspasedependentfaslsignalingpathway
AT kimdokyoung licochalconeainducesapoptosisinkbhumanoralcancercellsviaacaspasedependentfaslsignalingpathway
AT moonsungmin licochalconeainducesapoptosisinkbhumanoralcancercellsviaacaspasedependentfaslsignalingpathway
AT kimchunsung licochalconeainducesapoptosisinkbhumanoralcancercellsviaacaspasedependentfaslsignalingpathway
AT choseungsik licochalconeainducesapoptosisinkbhumanoralcancercellsviaacaspasedependentfaslsignalingpathway
AT yoongoo licochalconeainducesapoptosisinkbhumanoralcancercellsviaacaspasedependentfaslsignalingpathway
AT imheejeong licochalconeainducesapoptosisinkbhumanoralcancercellsviaacaspasedependentfaslsignalingpathway
AT youjaeseek licochalconeainducesapoptosisinkbhumanoralcancercellsviaacaspasedependentfaslsignalingpathway
AT ohjisu licochalconeainducesapoptosisinkbhumanoralcancercellsviaacaspasedependentfaslsignalingpathway
AT kimsugwan licochalconeainducesapoptosisinkbhumanoralcancercellsviaacaspasedependentfaslsignalingpathway