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Licochalcone A induces apoptosis in KB human oral cancer cells via a caspase-dependent FasL signaling pathway
Licochalcone A (Lico-A) is a natural phenol licorice compound with multiple bioactivities, including anti-inflammatory, anti-microbial, anti-fungal and osteogenesis-inducing properties. In the present study, we investigated the Lico-A-induced apoptotic effects and examined the associated apoptosis p...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983909/ https://www.ncbi.nlm.nih.gov/pubmed/24337492 http://dx.doi.org/10.3892/or.2013.2929 |
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author | KIM, JAE-SUNG PARK, MI-RA LEE, SOOK-YOUNG KIM, DO KYOUNG MOON, SUNG-MIN KIM, CHUN SUNG CHO, SEUNG SIK YOON, GOO IM, HEE-JEONG YOU, JAE-SEEK OH, JI-SU KIM, SU-GWAN |
author_facet | KIM, JAE-SUNG PARK, MI-RA LEE, SOOK-YOUNG KIM, DO KYOUNG MOON, SUNG-MIN KIM, CHUN SUNG CHO, SEUNG SIK YOON, GOO IM, HEE-JEONG YOU, JAE-SEEK OH, JI-SU KIM, SU-GWAN |
author_sort | KIM, JAE-SUNG |
collection | PubMed |
description | Licochalcone A (Lico-A) is a natural phenol licorice compound with multiple bioactivities, including anti-inflammatory, anti-microbial, anti-fungal and osteogenesis-inducing properties. In the present study, we investigated the Lico-A-induced apoptotic effects and examined the associated apoptosis pathway in KB human oral cancer cells. Lico-A decreased the number of viable KB oral cancer cells. However, Lico-A did not have an effect on primary normal human oral keratinocytes. In addition, the IC(50) value of Lico-A was determined to be ~50 μM following dose-dependent stimulation. KB oral cancer cells stimulated with Lico-A for 24 h showed chromatin condensation by DAPI staining, genomic DNA fragmentation by agarose gel electrophoresis and a gradually increased apoptotic cell population by FACS analysis. These data suggest that Lico-A induces apoptosis in KB oral cancer cells. Additionally, Lico-A-induced apoptosis in KB oral cancer cells was mediated by the expression of factor associated suicide ligand (FasL) and activated caspase-8 and −3 and poly(ADP-ribose) polymerase (PARP). Furthermore, in the KB oral cancer cells co-stimulation with a caspase inhibitor (Z-VAD-fmk) and Lico-A significantly abolished the apoptotic phenomena. Our findings demonstrated that Lico-A-induced apoptosis in KB oral cancer cells involves the extrinsic apoptotic signaling pathway, which involves a caspase-dependent FasL-mediated death receptor pathway. Our data suggest that Lico-A be developed as a chemotherapeutic agent for the management of oral cancer. |
format | Online Article Text |
id | pubmed-3983909 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-39839092014-04-11 Licochalcone A induces apoptosis in KB human oral cancer cells via a caspase-dependent FasL signaling pathway KIM, JAE-SUNG PARK, MI-RA LEE, SOOK-YOUNG KIM, DO KYOUNG MOON, SUNG-MIN KIM, CHUN SUNG CHO, SEUNG SIK YOON, GOO IM, HEE-JEONG YOU, JAE-SEEK OH, JI-SU KIM, SU-GWAN Oncol Rep Articles Licochalcone A (Lico-A) is a natural phenol licorice compound with multiple bioactivities, including anti-inflammatory, anti-microbial, anti-fungal and osteogenesis-inducing properties. In the present study, we investigated the Lico-A-induced apoptotic effects and examined the associated apoptosis pathway in KB human oral cancer cells. Lico-A decreased the number of viable KB oral cancer cells. However, Lico-A did not have an effect on primary normal human oral keratinocytes. In addition, the IC(50) value of Lico-A was determined to be ~50 μM following dose-dependent stimulation. KB oral cancer cells stimulated with Lico-A for 24 h showed chromatin condensation by DAPI staining, genomic DNA fragmentation by agarose gel electrophoresis and a gradually increased apoptotic cell population by FACS analysis. These data suggest that Lico-A induces apoptosis in KB oral cancer cells. Additionally, Lico-A-induced apoptosis in KB oral cancer cells was mediated by the expression of factor associated suicide ligand (FasL) and activated caspase-8 and −3 and poly(ADP-ribose) polymerase (PARP). Furthermore, in the KB oral cancer cells co-stimulation with a caspase inhibitor (Z-VAD-fmk) and Lico-A significantly abolished the apoptotic phenomena. Our findings demonstrated that Lico-A-induced apoptosis in KB oral cancer cells involves the extrinsic apoptotic signaling pathway, which involves a caspase-dependent FasL-mediated death receptor pathway. Our data suggest that Lico-A be developed as a chemotherapeutic agent for the management of oral cancer. D.A. Spandidos 2014-02 2013-12-16 /pmc/articles/PMC3983909/ /pubmed/24337492 http://dx.doi.org/10.3892/or.2013.2929 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Articles KIM, JAE-SUNG PARK, MI-RA LEE, SOOK-YOUNG KIM, DO KYOUNG MOON, SUNG-MIN KIM, CHUN SUNG CHO, SEUNG SIK YOON, GOO IM, HEE-JEONG YOU, JAE-SEEK OH, JI-SU KIM, SU-GWAN Licochalcone A induces apoptosis in KB human oral cancer cells via a caspase-dependent FasL signaling pathway |
title | Licochalcone A induces apoptosis in KB human oral cancer cells via a caspase-dependent FasL signaling pathway |
title_full | Licochalcone A induces apoptosis in KB human oral cancer cells via a caspase-dependent FasL signaling pathway |
title_fullStr | Licochalcone A induces apoptosis in KB human oral cancer cells via a caspase-dependent FasL signaling pathway |
title_full_unstemmed | Licochalcone A induces apoptosis in KB human oral cancer cells via a caspase-dependent FasL signaling pathway |
title_short | Licochalcone A induces apoptosis in KB human oral cancer cells via a caspase-dependent FasL signaling pathway |
title_sort | licochalcone a induces apoptosis in kb human oral cancer cells via a caspase-dependent fasl signaling pathway |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983909/ https://www.ncbi.nlm.nih.gov/pubmed/24337492 http://dx.doi.org/10.3892/or.2013.2929 |
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