An image-based, dual fluorescence reporter assay to evaluate the efficacy of shRNA for gene silencing at the single-cell level

RNA interference (RNAi) is widely used to suppress gene expression in a specific manner. The efficacy of RNAi is mainly dependent on the sequence of small interfering RNA (siRNA) in relation to the target mRNA. Although several algorithms have been developed for the design of siRNA, it is still diff...

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Detalles Bibliográficos
Autores principales: Kojima, Shin-ichiro, Borisy, Gary G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000Research 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983939/
https://www.ncbi.nlm.nih.gov/pubmed/24741441
http://dx.doi.org/10.12688/f1000research.3-60.v1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983939/
https://www.ncbi.nlm.nih.gov/pubmed/24741441
http://dx.doi.org/10.12688/f1000research.3-60.v1

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