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Lack of Clinical Manifestations in Asymptomatic Dengue Infection Is Attributed to Broad Down-Regulation and Selective Up-Regulation of Host Defence Response Genes

OBJECTIVES: Dengue represents one of the most serious life-threatening vector-borne infectious diseases that afflicts approximately 50 million people across the globe annually. Whilst symptomatic infections are frequently reported, asymptomatic dengue remains largely unnoticed. Therefore, we sought...

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Autores principales: Yeo, Adeline S. L., Azhar, Nur Atiqah, Yeow, Wanyi, Talbot, C. Conover, Khan, Mohammad Asif, Shankar, Esaki M., Rathakrishnan, Anusyah, Azizan, Azliyati, Wang, Seok Mui, Lee, Siew Kim, Fong, Mun Yik, Manikam, Rishya, Sekaran, Shamala Devi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3984081/
https://www.ncbi.nlm.nih.gov/pubmed/24727912
http://dx.doi.org/10.1371/journal.pone.0092240
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author Yeo, Adeline S. L.
Azhar, Nur Atiqah
Yeow, Wanyi
Talbot, C. Conover
Khan, Mohammad Asif
Shankar, Esaki M.
Rathakrishnan, Anusyah
Azizan, Azliyati
Wang, Seok Mui
Lee, Siew Kim
Fong, Mun Yik
Manikam, Rishya
Sekaran, Shamala Devi
author_facet Yeo, Adeline S. L.
Azhar, Nur Atiqah
Yeow, Wanyi
Talbot, C. Conover
Khan, Mohammad Asif
Shankar, Esaki M.
Rathakrishnan, Anusyah
Azizan, Azliyati
Wang, Seok Mui
Lee, Siew Kim
Fong, Mun Yik
Manikam, Rishya
Sekaran, Shamala Devi
author_sort Yeo, Adeline S. L.
collection PubMed
description OBJECTIVES: Dengue represents one of the most serious life-threatening vector-borne infectious diseases that afflicts approximately 50 million people across the globe annually. Whilst symptomatic infections are frequently reported, asymptomatic dengue remains largely unnoticed. Therefore, we sought to investigate the immune correlates conferring protection to individuals that remain clinically asymptomatic. METHODS: We determined the levels of neutralizing antibodies (nAbs) and gene expression profiles of host immune factors in individuals with asymptomatic infections, and whose cognate household members showed symptoms consistent to clinical dengue infection. RESULTS: We observed broad down-regulation of host defense response (innate, adaptive and matrix metalloprotease) genes in asymptomatic individuals as against symptomatic patients, with selective up-regulation of distinct genes that have been associated with protection. Selected down-regulated genes include: TNF α (TNF), IL8, C1S, factor B (CFB), IL2, IL3, IL4, IL5, IL8, IL9, IL10 and IL13, CD80, CD28, and IL18, MMP8, MMP10, MMP12, MMP15, MMP16, and MMP24. Selected up-regulated genes include: RANTES (CCL5), MIP-1α (CCL3L1/CCL3L3), MIP-1β (CCL4L1), TGFβ (TGFB), and TIMP1. CONCLUSION: Our findings highlight the potential association of certain host genes conferring protection against clinical dengue. These data are valuable to better explore the mysteries behind the hitherto poorly understood immunopathogenesis of subclinical dengue infection.
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spelling pubmed-39840812014-04-15 Lack of Clinical Manifestations in Asymptomatic Dengue Infection Is Attributed to Broad Down-Regulation and Selective Up-Regulation of Host Defence Response Genes Yeo, Adeline S. L. Azhar, Nur Atiqah Yeow, Wanyi Talbot, C. Conover Khan, Mohammad Asif Shankar, Esaki M. Rathakrishnan, Anusyah Azizan, Azliyati Wang, Seok Mui Lee, Siew Kim Fong, Mun Yik Manikam, Rishya Sekaran, Shamala Devi PLoS One Research Article OBJECTIVES: Dengue represents one of the most serious life-threatening vector-borne infectious diseases that afflicts approximately 50 million people across the globe annually. Whilst symptomatic infections are frequently reported, asymptomatic dengue remains largely unnoticed. Therefore, we sought to investigate the immune correlates conferring protection to individuals that remain clinically asymptomatic. METHODS: We determined the levels of neutralizing antibodies (nAbs) and gene expression profiles of host immune factors in individuals with asymptomatic infections, and whose cognate household members showed symptoms consistent to clinical dengue infection. RESULTS: We observed broad down-regulation of host defense response (innate, adaptive and matrix metalloprotease) genes in asymptomatic individuals as against symptomatic patients, with selective up-regulation of distinct genes that have been associated with protection. Selected down-regulated genes include: TNF α (TNF), IL8, C1S, factor B (CFB), IL2, IL3, IL4, IL5, IL8, IL9, IL10 and IL13, CD80, CD28, and IL18, MMP8, MMP10, MMP12, MMP15, MMP16, and MMP24. Selected up-regulated genes include: RANTES (CCL5), MIP-1α (CCL3L1/CCL3L3), MIP-1β (CCL4L1), TGFβ (TGFB), and TIMP1. CONCLUSION: Our findings highlight the potential association of certain host genes conferring protection against clinical dengue. These data are valuable to better explore the mysteries behind the hitherto poorly understood immunopathogenesis of subclinical dengue infection. Public Library of Science 2014-04-11 /pmc/articles/PMC3984081/ /pubmed/24727912 http://dx.doi.org/10.1371/journal.pone.0092240 Text en © 2014 Yeo et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Yeo, Adeline S. L.
Azhar, Nur Atiqah
Yeow, Wanyi
Talbot, C. Conover
Khan, Mohammad Asif
Shankar, Esaki M.
Rathakrishnan, Anusyah
Azizan, Azliyati
Wang, Seok Mui
Lee, Siew Kim
Fong, Mun Yik
Manikam, Rishya
Sekaran, Shamala Devi
Lack of Clinical Manifestations in Asymptomatic Dengue Infection Is Attributed to Broad Down-Regulation and Selective Up-Regulation of Host Defence Response Genes
title Lack of Clinical Manifestations in Asymptomatic Dengue Infection Is Attributed to Broad Down-Regulation and Selective Up-Regulation of Host Defence Response Genes
title_full Lack of Clinical Manifestations in Asymptomatic Dengue Infection Is Attributed to Broad Down-Regulation and Selective Up-Regulation of Host Defence Response Genes
title_fullStr Lack of Clinical Manifestations in Asymptomatic Dengue Infection Is Attributed to Broad Down-Regulation and Selective Up-Regulation of Host Defence Response Genes
title_full_unstemmed Lack of Clinical Manifestations in Asymptomatic Dengue Infection Is Attributed to Broad Down-Regulation and Selective Up-Regulation of Host Defence Response Genes
title_short Lack of Clinical Manifestations in Asymptomatic Dengue Infection Is Attributed to Broad Down-Regulation and Selective Up-Regulation of Host Defence Response Genes
title_sort lack of clinical manifestations in asymptomatic dengue infection is attributed to broad down-regulation and selective up-regulation of host defence response genes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3984081/
https://www.ncbi.nlm.nih.gov/pubmed/24727912
http://dx.doi.org/10.1371/journal.pone.0092240
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