Cargando…

Selected Lactic Acid-Producing Bacterial Isolates with the Capacity to Reduce Salmonella Translocation and Virulence Gene Expression in Chickens

BACKGROUND: Probiotics have been used to control Salmonella colonization/infection in chickens. Yet the mechanisms of probiotic effects are not fully understood. This study has characterized our previously-selected lactic acid-producing bacterial (LAB) isolates for controlling Salmonella infection i...

Descripción completa

Detalles Bibliográficos
Autores principales: Yang, Xiaojian, Brisbin, Jennifer, Yu, Hai, Wang, Qi, Yin, Fugui, Zhang, Yonggang, Sabour, Parviz, Sharif, Shayan, Gong, Joshua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3984083/
https://www.ncbi.nlm.nih.gov/pubmed/24728092
http://dx.doi.org/10.1371/journal.pone.0093022
_version_ 1782311394757050368
author Yang, Xiaojian
Brisbin, Jennifer
Yu, Hai
Wang, Qi
Yin, Fugui
Zhang, Yonggang
Sabour, Parviz
Sharif, Shayan
Gong, Joshua
author_facet Yang, Xiaojian
Brisbin, Jennifer
Yu, Hai
Wang, Qi
Yin, Fugui
Zhang, Yonggang
Sabour, Parviz
Sharif, Shayan
Gong, Joshua
author_sort Yang, Xiaojian
collection PubMed
description BACKGROUND: Probiotics have been used to control Salmonella colonization/infection in chickens. Yet the mechanisms of probiotic effects are not fully understood. This study has characterized our previously-selected lactic acid-producing bacterial (LAB) isolates for controlling Salmonella infection in chickens, particularly the mechanism underlying the control. METHODOLOGY/PRINCIPAL FINDINGS: In vitro studies were conducted to characterize 14 LAB isolates for their tolerance to low pH (2.0) and high bile salt (0.3–1.5%) and susceptibility to antibiotics. Three chicken infection trials were subsequently carried out to evaluate four of the isolates for reducing the burden of Salmonella enterica serovar Typhimurium in the broiler cecum. Chicks were gavaged with LAB cultures (10(6–7) CFU/chick) or phosphate-buffered saline (PBS) at 1 day of age followed by Salmonella challenge (10(4) CFU/chick) next day. Samples of cecal digesta, spleen, and liver were examined for Salmonella counts on days 1, 3, or 4 post-challenge. Salmonella in the cecum from Trial 3 was also assessed for the expression of ten virulence genes located in its pathogenicity island-1 (SPI-1). These genes play a role in Salmonella intestinal invasion. Tested LAB isolates (individuals or mixed cultures) were unable to lower Salmonella burden in the chicken cecum, but able to attenuate Salmonella infection in the spleen and liver. The LAB treatments also reduced almost all SPI-1 virulence gene expression (9 out of 10) in the chicken cecum, particularly at the low dose. In vitro treatment with the extracellular culture fluid from a LAB culture also down-regulated most SPI-1 virulence gene expression. CONCLUSIONS/SIGNIFICANCE: The possible correlation between attenuation of Salmonella infection in the chicken spleen and liver and reduction of Salmonella SPI-1 virulence gene expression in the chicken cecum by LAB isolates is a new observation. Suppression of Salmonella virulence gene expression in vivo can be one of the strategies for controlling Salmonella infection in chickens.
format Online
Article
Text
id pubmed-3984083
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-39840832014-04-15 Selected Lactic Acid-Producing Bacterial Isolates with the Capacity to Reduce Salmonella Translocation and Virulence Gene Expression in Chickens Yang, Xiaojian Brisbin, Jennifer Yu, Hai Wang, Qi Yin, Fugui Zhang, Yonggang Sabour, Parviz Sharif, Shayan Gong, Joshua PLoS One Research Article BACKGROUND: Probiotics have been used to control Salmonella colonization/infection in chickens. Yet the mechanisms of probiotic effects are not fully understood. This study has characterized our previously-selected lactic acid-producing bacterial (LAB) isolates for controlling Salmonella infection in chickens, particularly the mechanism underlying the control. METHODOLOGY/PRINCIPAL FINDINGS: In vitro studies were conducted to characterize 14 LAB isolates for their tolerance to low pH (2.0) and high bile salt (0.3–1.5%) and susceptibility to antibiotics. Three chicken infection trials were subsequently carried out to evaluate four of the isolates for reducing the burden of Salmonella enterica serovar Typhimurium in the broiler cecum. Chicks were gavaged with LAB cultures (10(6–7) CFU/chick) or phosphate-buffered saline (PBS) at 1 day of age followed by Salmonella challenge (10(4) CFU/chick) next day. Samples of cecal digesta, spleen, and liver were examined for Salmonella counts on days 1, 3, or 4 post-challenge. Salmonella in the cecum from Trial 3 was also assessed for the expression of ten virulence genes located in its pathogenicity island-1 (SPI-1). These genes play a role in Salmonella intestinal invasion. Tested LAB isolates (individuals or mixed cultures) were unable to lower Salmonella burden in the chicken cecum, but able to attenuate Salmonella infection in the spleen and liver. The LAB treatments also reduced almost all SPI-1 virulence gene expression (9 out of 10) in the chicken cecum, particularly at the low dose. In vitro treatment with the extracellular culture fluid from a LAB culture also down-regulated most SPI-1 virulence gene expression. CONCLUSIONS/SIGNIFICANCE: The possible correlation between attenuation of Salmonella infection in the chicken spleen and liver and reduction of Salmonella SPI-1 virulence gene expression in the chicken cecum by LAB isolates is a new observation. Suppression of Salmonella virulence gene expression in vivo can be one of the strategies for controlling Salmonella infection in chickens. Public Library of Science 2014-04-11 /pmc/articles/PMC3984083/ /pubmed/24728092 http://dx.doi.org/10.1371/journal.pone.0093022 Text en © 2014 Crown Copyright
spellingShingle Research Article
Yang, Xiaojian
Brisbin, Jennifer
Yu, Hai
Wang, Qi
Yin, Fugui
Zhang, Yonggang
Sabour, Parviz
Sharif, Shayan
Gong, Joshua
Selected Lactic Acid-Producing Bacterial Isolates with the Capacity to Reduce Salmonella Translocation and Virulence Gene Expression in Chickens
title Selected Lactic Acid-Producing Bacterial Isolates with the Capacity to Reduce Salmonella Translocation and Virulence Gene Expression in Chickens
title_full Selected Lactic Acid-Producing Bacterial Isolates with the Capacity to Reduce Salmonella Translocation and Virulence Gene Expression in Chickens
title_fullStr Selected Lactic Acid-Producing Bacterial Isolates with the Capacity to Reduce Salmonella Translocation and Virulence Gene Expression in Chickens
title_full_unstemmed Selected Lactic Acid-Producing Bacterial Isolates with the Capacity to Reduce Salmonella Translocation and Virulence Gene Expression in Chickens
title_short Selected Lactic Acid-Producing Bacterial Isolates with the Capacity to Reduce Salmonella Translocation and Virulence Gene Expression in Chickens
title_sort selected lactic acid-producing bacterial isolates with the capacity to reduce salmonella translocation and virulence gene expression in chickens
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3984083/
https://www.ncbi.nlm.nih.gov/pubmed/24728092
http://dx.doi.org/10.1371/journal.pone.0093022
work_keys_str_mv AT yangxiaojian selectedlacticacidproducingbacterialisolateswiththecapacitytoreducesalmonellatranslocationandvirulencegeneexpressioninchickens
AT brisbinjennifer selectedlacticacidproducingbacterialisolateswiththecapacitytoreducesalmonellatranslocationandvirulencegeneexpressioninchickens
AT yuhai selectedlacticacidproducingbacterialisolateswiththecapacitytoreducesalmonellatranslocationandvirulencegeneexpressioninchickens
AT wangqi selectedlacticacidproducingbacterialisolateswiththecapacitytoreducesalmonellatranslocationandvirulencegeneexpressioninchickens
AT yinfugui selectedlacticacidproducingbacterialisolateswiththecapacitytoreducesalmonellatranslocationandvirulencegeneexpressioninchickens
AT zhangyonggang selectedlacticacidproducingbacterialisolateswiththecapacitytoreducesalmonellatranslocationandvirulencegeneexpressioninchickens
AT sabourparviz selectedlacticacidproducingbacterialisolateswiththecapacitytoreducesalmonellatranslocationandvirulencegeneexpressioninchickens
AT sharifshayan selectedlacticacidproducingbacterialisolateswiththecapacitytoreducesalmonellatranslocationandvirulencegeneexpressioninchickens
AT gongjoshua selectedlacticacidproducingbacterialisolateswiththecapacitytoreducesalmonellatranslocationandvirulencegeneexpressioninchickens