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COLD-PCR Amplification of Bisulfite-Converted DNA Allows the Enrichment and Sequencing of Rare Un-Methylated Genomic Regions

Aberrant hypo-methylation of DNA is evident in a range of human diseases including cancer and diabetes. Development of sensitive assays capable of detecting traces of un-methylated DNA within methylated samples can be useful in several situations. Here we describe a new approach, fast-COLD-MS-PCR, w...

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Detalles Bibliográficos
Autores principales:	Castellanos-Rizaldos, Elena, Milbury, Coren A., Karatza, Elli, Chen, Clark C., Makrigiorgos, G. Mike, Merewood, Anne
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2014
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3984089/ https://www.ncbi.nlm.nih.gov/pubmed/24728321 http://dx.doi.org/10.1371/journal.pone.0094103

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