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Developmental Stage-Specific Regulation of the Circadian Clock by Temperature in Zebrafish
The circadian clock enables animals to adapt their physiology and behaviour in anticipation of the day-night cycle. Light and temperature represent two key environmental timing cues (zeitgebers) able to reset this mechanism and so maintain its synchronization with the environmental cycle. One key ch...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3984786/ https://www.ncbi.nlm.nih.gov/pubmed/24791007 http://dx.doi.org/10.1155/2014/930308 |
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author | Lahiri, Kajori Froehlich, Nadine Heyd, Andreas Foulkes, Nicholas S. Vallone, Daniela |
author_facet | Lahiri, Kajori Froehlich, Nadine Heyd, Andreas Foulkes, Nicholas S. Vallone, Daniela |
author_sort | Lahiri, Kajori |
collection | PubMed |
description | The circadian clock enables animals to adapt their physiology and behaviour in anticipation of the day-night cycle. Light and temperature represent two key environmental timing cues (zeitgebers) able to reset this mechanism and so maintain its synchronization with the environmental cycle. One key challenge is to unravel how the regulation of the clock by zeitgebers matures during early development. The zebrafish is an ideal model for studying circadian clock ontogeny since the process of development occurs ex utero in an optically transparent chorion and many tools are available for genetic analysis. However, the role played by temperature in regulating the clock during zebrafish development is poorly understood. Here, we have established a clock-regulated luciferase reporter transgenic zebrafish line (Tg (−3.1) per1b::luc) to study the effects of temperature on clock entrainment. We reveal that under complete darkness, from an early developmental stage onwards (48 to 72 hpf), exposure to temperature cycles is a prerequisite for the establishment of self-sustaining rhythms of zfper1b, zfaanat2, and zfirbp expression and also for circadian cell cycle rhythms. Furthermore, we show that following the 5–9 somite stage, the expression of zfper1b is regulated by acute temperature shifts. |
format | Online Article Text |
id | pubmed-3984786 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-39847862014-04-30 Developmental Stage-Specific Regulation of the Circadian Clock by Temperature in Zebrafish Lahiri, Kajori Froehlich, Nadine Heyd, Andreas Foulkes, Nicholas S. Vallone, Daniela Biomed Res Int Research Article The circadian clock enables animals to adapt their physiology and behaviour in anticipation of the day-night cycle. Light and temperature represent two key environmental timing cues (zeitgebers) able to reset this mechanism and so maintain its synchronization with the environmental cycle. One key challenge is to unravel how the regulation of the clock by zeitgebers matures during early development. The zebrafish is an ideal model for studying circadian clock ontogeny since the process of development occurs ex utero in an optically transparent chorion and many tools are available for genetic analysis. However, the role played by temperature in regulating the clock during zebrafish development is poorly understood. Here, we have established a clock-regulated luciferase reporter transgenic zebrafish line (Tg (−3.1) per1b::luc) to study the effects of temperature on clock entrainment. We reveal that under complete darkness, from an early developmental stage onwards (48 to 72 hpf), exposure to temperature cycles is a prerequisite for the establishment of self-sustaining rhythms of zfper1b, zfaanat2, and zfirbp expression and also for circadian cell cycle rhythms. Furthermore, we show that following the 5–9 somite stage, the expression of zfper1b is regulated by acute temperature shifts. Hindawi Publishing Corporation 2014 2014-03-27 /pmc/articles/PMC3984786/ /pubmed/24791007 http://dx.doi.org/10.1155/2014/930308 Text en Copyright © 2014 Kajori Lahiri et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Lahiri, Kajori Froehlich, Nadine Heyd, Andreas Foulkes, Nicholas S. Vallone, Daniela Developmental Stage-Specific Regulation of the Circadian Clock by Temperature in Zebrafish |
title | Developmental Stage-Specific Regulation of the Circadian Clock by Temperature in Zebrafish |
title_full | Developmental Stage-Specific Regulation of the Circadian Clock by Temperature in Zebrafish |
title_fullStr | Developmental Stage-Specific Regulation of the Circadian Clock by Temperature in Zebrafish |
title_full_unstemmed | Developmental Stage-Specific Regulation of the Circadian Clock by Temperature in Zebrafish |
title_short | Developmental Stage-Specific Regulation of the Circadian Clock by Temperature in Zebrafish |
title_sort | developmental stage-specific regulation of the circadian clock by temperature in zebrafish |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3984786/ https://www.ncbi.nlm.nih.gov/pubmed/24791007 http://dx.doi.org/10.1155/2014/930308 |
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