Cargando…
Growth Phase-Dependent Proteomes of the Malaysian Isolated Lactococcus lactis Dairy Strain M4 Using Label-Free Qualitative Shotgun Proteomics Analysis
Lactococcus lactis is the most studied mesophilic fermentative lactic acid bacterium. It is used extensively in the food industry and plays a pivotal role as a cell factory and also as vaccine delivery platforms. The proteome of the Malaysian isolated L. lactis M4 dairy strain, obtained from the mil...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3984853/ https://www.ncbi.nlm.nih.gov/pubmed/24982972 http://dx.doi.org/10.1155/2014/642891 |
_version_ | 1782311504894230528 |
---|---|
author | Yap, Theresa Wan Chen Rabu, Amir Abu Bakar, Farah Diba Abdul Rahim, Raha Mahadi, Nor Muhammad Illias, Rosli Md. Abdul Murad, Abdul Munir |
author_facet | Yap, Theresa Wan Chen Rabu, Amir Abu Bakar, Farah Diba Abdul Rahim, Raha Mahadi, Nor Muhammad Illias, Rosli Md. Abdul Murad, Abdul Munir |
author_sort | Yap, Theresa Wan Chen |
collection | PubMed |
description | Lactococcus lactis is the most studied mesophilic fermentative lactic acid bacterium. It is used extensively in the food industry and plays a pivotal role as a cell factory and also as vaccine delivery platforms. The proteome of the Malaysian isolated L. lactis M4 dairy strain, obtained from the milk of locally bred cows, was studied to elucidate the physiological changes occurring between the growth phases of this bacterium. In this study, ultraperformance liquid chromatography nanoflow electrospray ionization tandem mass spectrometry (UPLC- nano-ESI-MS(E)) approach was used for qualitative proteomic analysis. A total of 100 and 121 proteins were identified from the midexponential and early stationary growth phases, respectively, of the L. lactis strain M4. During the exponential phase, the most important reaction was the generation of sufficient energy, whereas, in the early stationary phase, the metabolic energy pathways decreased and the biosynthesis of proteins became more important. Thus, the metabolism of the cells shifted from energy production in the exponential phase to the synthesis of macromolecules in the stationary phase. The resultant proteomes are essential in providing an improved view of the cellular machinery of L. lactis during the transition of growth phases and hence provide insight into various biotechnological applications. |
format | Online Article Text |
id | pubmed-3984853 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-39848532014-06-30 Growth Phase-Dependent Proteomes of the Malaysian Isolated Lactococcus lactis Dairy Strain M4 Using Label-Free Qualitative Shotgun Proteomics Analysis Yap, Theresa Wan Chen Rabu, Amir Abu Bakar, Farah Diba Abdul Rahim, Raha Mahadi, Nor Muhammad Illias, Rosli Md. Abdul Murad, Abdul Munir ScientificWorldJournal Research Article Lactococcus lactis is the most studied mesophilic fermentative lactic acid bacterium. It is used extensively in the food industry and plays a pivotal role as a cell factory and also as vaccine delivery platforms. The proteome of the Malaysian isolated L. lactis M4 dairy strain, obtained from the milk of locally bred cows, was studied to elucidate the physiological changes occurring between the growth phases of this bacterium. In this study, ultraperformance liquid chromatography nanoflow electrospray ionization tandem mass spectrometry (UPLC- nano-ESI-MS(E)) approach was used for qualitative proteomic analysis. A total of 100 and 121 proteins were identified from the midexponential and early stationary growth phases, respectively, of the L. lactis strain M4. During the exponential phase, the most important reaction was the generation of sufficient energy, whereas, in the early stationary phase, the metabolic energy pathways decreased and the biosynthesis of proteins became more important. Thus, the metabolism of the cells shifted from energy production in the exponential phase to the synthesis of macromolecules in the stationary phase. The resultant proteomes are essential in providing an improved view of the cellular machinery of L. lactis during the transition of growth phases and hence provide insight into various biotechnological applications. Hindawi Publishing Corporation 2014 2014-03-25 /pmc/articles/PMC3984853/ /pubmed/24982972 http://dx.doi.org/10.1155/2014/642891 Text en Copyright © 2014 Theresa Wan Chen Yap et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Yap, Theresa Wan Chen Rabu, Amir Abu Bakar, Farah Diba Abdul Rahim, Raha Mahadi, Nor Muhammad Illias, Rosli Md. Abdul Murad, Abdul Munir Growth Phase-Dependent Proteomes of the Malaysian Isolated Lactococcus lactis Dairy Strain M4 Using Label-Free Qualitative Shotgun Proteomics Analysis |
title | Growth Phase-Dependent Proteomes of the Malaysian Isolated Lactococcus lactis Dairy Strain M4 Using Label-Free Qualitative Shotgun Proteomics Analysis |
title_full | Growth Phase-Dependent Proteomes of the Malaysian Isolated Lactococcus lactis Dairy Strain M4 Using Label-Free Qualitative Shotgun Proteomics Analysis |
title_fullStr | Growth Phase-Dependent Proteomes of the Malaysian Isolated Lactococcus lactis Dairy Strain M4 Using Label-Free Qualitative Shotgun Proteomics Analysis |
title_full_unstemmed | Growth Phase-Dependent Proteomes of the Malaysian Isolated Lactococcus lactis Dairy Strain M4 Using Label-Free Qualitative Shotgun Proteomics Analysis |
title_short | Growth Phase-Dependent Proteomes of the Malaysian Isolated Lactococcus lactis Dairy Strain M4 Using Label-Free Qualitative Shotgun Proteomics Analysis |
title_sort | growth phase-dependent proteomes of the malaysian isolated lactococcus lactis dairy strain m4 using label-free qualitative shotgun proteomics analysis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3984853/ https://www.ncbi.nlm.nih.gov/pubmed/24982972 http://dx.doi.org/10.1155/2014/642891 |
work_keys_str_mv | AT yaptheresawanchen growthphasedependentproteomesofthemalaysianisolatedlactococcuslactisdairystrainm4usinglabelfreequalitativeshotgunproteomicsanalysis AT rabuamir growthphasedependentproteomesofthemalaysianisolatedlactococcuslactisdairystrainm4usinglabelfreequalitativeshotgunproteomicsanalysis AT abubakarfarahdiba growthphasedependentproteomesofthemalaysianisolatedlactococcuslactisdairystrainm4usinglabelfreequalitativeshotgunproteomicsanalysis AT abdulrahimraha growthphasedependentproteomesofthemalaysianisolatedlactococcuslactisdairystrainm4usinglabelfreequalitativeshotgunproteomicsanalysis AT mahadinormuhammad growthphasedependentproteomesofthemalaysianisolatedlactococcuslactisdairystrainm4usinglabelfreequalitativeshotgunproteomicsanalysis AT illiasroslimd growthphasedependentproteomesofthemalaysianisolatedlactococcuslactisdairystrainm4usinglabelfreequalitativeshotgunproteomicsanalysis AT abdulmuradabdulmunir growthphasedependentproteomesofthemalaysianisolatedlactococcuslactisdairystrainm4usinglabelfreequalitativeshotgunproteomicsanalysis |