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Optimization of Conditions for In Vitro Culture of the Microphallid Digenean Gynaecotyla adunca

In vitro cultivation of digeneans would aid the development of effective treatments and studies of the biology of the parasites. The goal of this study was to optimize culture conditions for the trematode, Gynaecotyla adunca. Metacercariae of the parasite from fiddler crabs, Uca pugnax, excysted in...

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Detalles Bibliográficos
Autores principales: West, Jenna, Mitchell, Alexandra, Pung, Oscar J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3984872/
https://www.ncbi.nlm.nih.gov/pubmed/24795817
http://dx.doi.org/10.1155/2014/382153
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author West, Jenna
Mitchell, Alexandra
Pung, Oscar J.
author_facet West, Jenna
Mitchell, Alexandra
Pung, Oscar J.
author_sort West, Jenna
collection PubMed
description In vitro cultivation of digeneans would aid the development of effective treatments and studies of the biology of the parasites. The goal of this study was to optimize culture conditions for the trematode, Gynaecotyla adunca. Metacercariae of the parasite from fiddler crabs, Uca pugnax, excysted in trypsin, were incubated overnight to permit fertilization, and were cultured in different conditions to find those that resulted in maximum worm longevity and egg production. When cultured in media lacking serum, worms lived longer in Hanks balanced salt solution and Dulbecco's Modified Eagle medium/F-12 (DME/F-12) than in RPMI-1640 but produced the most eggs in DME/F-12. Worm longevity and egg production increased when worms were grown in DME/F-12 supplemented with 20% chicken, horse, or newborn calf serum but the greatest number of eggs was deposited in cultures containing horse or chicken serum. Horse serum was chosen over chicken serum due to the formation of a precipitate in chicken serum. The optimal concentration of horse serum with respect to egg production ranged from 5 to 20%. Infectivity of eggs deposited by worms in culture was tested by feeding eggs to mud snails, Ilyanassa obsoleta. None of these snails produced G. adunca cercariae.
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spelling pubmed-39848722014-05-04 Optimization of Conditions for In Vitro Culture of the Microphallid Digenean Gynaecotyla adunca West, Jenna Mitchell, Alexandra Pung, Oscar J. J Parasitol Res Research Article In vitro cultivation of digeneans would aid the development of effective treatments and studies of the biology of the parasites. The goal of this study was to optimize culture conditions for the trematode, Gynaecotyla adunca. Metacercariae of the parasite from fiddler crabs, Uca pugnax, excysted in trypsin, were incubated overnight to permit fertilization, and were cultured in different conditions to find those that resulted in maximum worm longevity and egg production. When cultured in media lacking serum, worms lived longer in Hanks balanced salt solution and Dulbecco's Modified Eagle medium/F-12 (DME/F-12) than in RPMI-1640 but produced the most eggs in DME/F-12. Worm longevity and egg production increased when worms were grown in DME/F-12 supplemented with 20% chicken, horse, or newborn calf serum but the greatest number of eggs was deposited in cultures containing horse or chicken serum. Horse serum was chosen over chicken serum due to the formation of a precipitate in chicken serum. The optimal concentration of horse serum with respect to egg production ranged from 5 to 20%. Infectivity of eggs deposited by worms in culture was tested by feeding eggs to mud snails, Ilyanassa obsoleta. None of these snails produced G. adunca cercariae. Hindawi Publishing Corporation 2014 2014-03-25 /pmc/articles/PMC3984872/ /pubmed/24795817 http://dx.doi.org/10.1155/2014/382153 Text en Copyright © 2014 Jenna West et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
West, Jenna
Mitchell, Alexandra
Pung, Oscar J.
Optimization of Conditions for In Vitro Culture of the Microphallid Digenean Gynaecotyla adunca
title Optimization of Conditions for In Vitro Culture of the Microphallid Digenean Gynaecotyla adunca
title_full Optimization of Conditions for In Vitro Culture of the Microphallid Digenean Gynaecotyla adunca
title_fullStr Optimization of Conditions for In Vitro Culture of the Microphallid Digenean Gynaecotyla adunca
title_full_unstemmed Optimization of Conditions for In Vitro Culture of the Microphallid Digenean Gynaecotyla adunca
title_short Optimization of Conditions for In Vitro Culture of the Microphallid Digenean Gynaecotyla adunca
title_sort optimization of conditions for in vitro culture of the microphallid digenean gynaecotyla adunca
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3984872/
https://www.ncbi.nlm.nih.gov/pubmed/24795817
http://dx.doi.org/10.1155/2014/382153
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