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Vancomycin iontophoresis of allograft bone
OBJECTIVES: The most concerning infection of allografts and operative procedures is methicillin resistant Staphylococcus aureus (MRSA) and no current iontophoresed antibiotics effectively combat this microbe. It was initially hypothesised that iontophoresis of vancomycin through bone would not be ef...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
British Editorial Society of Bone and Joint Surgery
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3985199/ https://www.ncbi.nlm.nih.gov/pubmed/24729101 http://dx.doi.org/10.1302/2046-3758.34.2000223 |
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author | Edmondson, M. C. Day, R. Wood, D. |
author_facet | Edmondson, M. C. Day, R. Wood, D. |
author_sort | Edmondson, M. C. |
collection | PubMed |
description | OBJECTIVES: The most concerning infection of allografts and operative procedures is methicillin resistant Staphylococcus aureus (MRSA) and no current iontophoresed antibiotics effectively combat this microbe. It was initially hypothesised that iontophoresis of vancomycin through bone would not be effective due to its large molecular size and lack of charge. The aim of this study was to determine whether this was a viable procedure and to find the optimum conditions for its use. METHODS: An iontophoresis cell was set up with varying concentrations of Vancomycin within the medulla of a section of sheep tibia, sealed from an external saline solution. The cell was run for varying times, Vancomycin concentrations and voltages, to gain information on optimisation of conditions for impregnating the graft. Each graft was then sectioned and dust ground from the exposed surface. The dust was serially washed to extract the Vancomycin and concentrations measured and plotted for all variables tested. RESULTS: Vancomycin was successfully delivered and impregnated to the graft using the iontophoresis technique. The first order fit to the whole data set gave a significant result (p = 0.0233), with a significant concentration (p = 0.02774) component. The time component was the next most significant (p = 0.0597), but did not exceed the 95% confidence level. CONCLUSIONS: Iontophoresis is an effective method for delivering Vancomycin to allograft bone. The concentrations of the vancomycin solution affected the bone concentration, but results were highly variable. Further study should be done on the effectiveness of delivering different antibiotics using this method. |
format | Online Article Text |
id | pubmed-3985199 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | British Editorial Society of Bone and Joint Surgery |
record_format | MEDLINE/PubMed |
spelling | pubmed-39851992014-04-21 Vancomycin iontophoresis of allograft bone Edmondson, M. C. Day, R. Wood, D. Bone Joint Res Oncology OBJECTIVES: The most concerning infection of allografts and operative procedures is methicillin resistant Staphylococcus aureus (MRSA) and no current iontophoresed antibiotics effectively combat this microbe. It was initially hypothesised that iontophoresis of vancomycin through bone would not be effective due to its large molecular size and lack of charge. The aim of this study was to determine whether this was a viable procedure and to find the optimum conditions for its use. METHODS: An iontophoresis cell was set up with varying concentrations of Vancomycin within the medulla of a section of sheep tibia, sealed from an external saline solution. The cell was run for varying times, Vancomycin concentrations and voltages, to gain information on optimisation of conditions for impregnating the graft. Each graft was then sectioned and dust ground from the exposed surface. The dust was serially washed to extract the Vancomycin and concentrations measured and plotted for all variables tested. RESULTS: Vancomycin was successfully delivered and impregnated to the graft using the iontophoresis technique. The first order fit to the whole data set gave a significant result (p = 0.0233), with a significant concentration (p = 0.02774) component. The time component was the next most significant (p = 0.0597), but did not exceed the 95% confidence level. CONCLUSIONS: Iontophoresis is an effective method for delivering Vancomycin to allograft bone. The concentrations of the vancomycin solution affected the bone concentration, but results were highly variable. Further study should be done on the effectiveness of delivering different antibiotics using this method. British Editorial Society of Bone and Joint Surgery 2014-04-01 /pmc/articles/PMC3985199/ /pubmed/24729101 http://dx.doi.org/10.1302/2046-3758.34.2000223 Text en ©2014 The British Editorial Society of Bone & Joint Surgery ©2014 The British Editorial Society of Bone & Joint Surgery. This is an open-access article distributed under the terms of the Creative Commons Attributions licence, which permits unrestricted use, distribution, and reproduction in any medium, but not for commercial gain, provided the original author and source are credited. |
spellingShingle | Oncology Edmondson, M. C. Day, R. Wood, D. Vancomycin iontophoresis of allograft bone |
title | Vancomycin iontophoresis of allograft bone |
title_full | Vancomycin iontophoresis of allograft bone |
title_fullStr | Vancomycin iontophoresis of allograft bone |
title_full_unstemmed | Vancomycin iontophoresis of allograft bone |
title_short | Vancomycin iontophoresis of allograft bone |
title_sort | vancomycin iontophoresis of allograft bone |
topic | Oncology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3985199/ https://www.ncbi.nlm.nih.gov/pubmed/24729101 http://dx.doi.org/10.1302/2046-3758.34.2000223 |
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