Cargando…

Propofol Attenuates Toxic Oxidative Stress by CCl(4) in Liver Mitochondria and Blood in Rat

Anti-oxidant effects of propofol (2, 6-diisopropylphenol) were evaluated agains carbon tetrachloridet CCl(4) -induced oxidative stress in rat liver. 30 male rats were equally divided in to 6 groups (5 rats each). Group I (control), while Group II was given CCl(4) (3 mL /Kg/day, IP). Animals of Group...

Descripción completa

Detalles Bibliográficos
Autores principales: Ranjbar, Akram, Sharifzadeh, Mohammad, Karimi, Jamshid, Tavilani, Heidar, Baeeri, Maryam, Heidary shayesteh, Tavakol, Abdollahi, Mohammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shaheed Beheshti University of Medical Sciences 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3985254/
https://www.ncbi.nlm.nih.gov/pubmed/24734078
_version_ 1782311547275575296
author Ranjbar, Akram
Sharifzadeh, Mohammad
Karimi, Jamshid
Tavilani, Heidar
Baeeri, Maryam
Heidary shayesteh, Tavakol
Abdollahi, Mohammad
author_facet Ranjbar, Akram
Sharifzadeh, Mohammad
Karimi, Jamshid
Tavilani, Heidar
Baeeri, Maryam
Heidary shayesteh, Tavakol
Abdollahi, Mohammad
author_sort Ranjbar, Akram
collection PubMed
description Anti-oxidant effects of propofol (2, 6-diisopropylphenol) were evaluated agains carbon tetrachloridet CCl(4) -induced oxidative stress in rat liver. 30 male rats were equally divided in to 6 groups (5 rats each). Group I (control), while Group II was given CCl(4) (3 mL /Kg/day, IP). Animals of Groups III received only propofol (10 mg/Kg/day, IP). Group IV was given propofol+ CCl(4). Group V was administered vitamin E (alpha-tocopherol acetate 15 mg/Kg/day, SC) .Animals of Group VII received alpha-tocopherol acetate + CCl(4) once daily for two weeks. After treatment, blood and liver mitochondria were isolated. Anti-oxidant enzymes activity such as glutathione peroxidase (GPx), superoxide dismutase (SOD) and oxidative stress marker such as reduced glutathione (GSH) and lipid peroxidation (LPO) concentration were measured. Oxidative stress induced with CCl(4) in liver mitochondria was evident by a significant increase in enzymatic activities of GPx, SOD, and LPO and decreased of GSH and vailability of mitochondria. Propofol and vitamin E restored CCl(4)-induced changes in GSH, GPx, SOD and LPO in blood and liver mitochondria. CCl(4) decreased viability of mitochondria that was recovered by propofol and vitamin E. It is concluded that oxidative damage is the mechanism of toxicity of CCl(4) in the mitochondria that can be recovered by propofol comparable to vitamin E.
format Online
Article
Text
id pubmed-3985254
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Shaheed Beheshti University of Medical Sciences
record_format MEDLINE/PubMed
spelling pubmed-39852542014-04-14 Propofol Attenuates Toxic Oxidative Stress by CCl(4) in Liver Mitochondria and Blood in Rat Ranjbar, Akram Sharifzadeh, Mohammad Karimi, Jamshid Tavilani, Heidar Baeeri, Maryam Heidary shayesteh, Tavakol Abdollahi, Mohammad Iran J Pharm Res Original Article Anti-oxidant effects of propofol (2, 6-diisopropylphenol) were evaluated agains carbon tetrachloridet CCl(4) -induced oxidative stress in rat liver. 30 male rats were equally divided in to 6 groups (5 rats each). Group I (control), while Group II was given CCl(4) (3 mL /Kg/day, IP). Animals of Groups III received only propofol (10 mg/Kg/day, IP). Group IV was given propofol+ CCl(4). Group V was administered vitamin E (alpha-tocopherol acetate 15 mg/Kg/day, SC) .Animals of Group VII received alpha-tocopherol acetate + CCl(4) once daily for two weeks. After treatment, blood and liver mitochondria were isolated. Anti-oxidant enzymes activity such as glutathione peroxidase (GPx), superoxide dismutase (SOD) and oxidative stress marker such as reduced glutathione (GSH) and lipid peroxidation (LPO) concentration were measured. Oxidative stress induced with CCl(4) in liver mitochondria was evident by a significant increase in enzymatic activities of GPx, SOD, and LPO and decreased of GSH and vailability of mitochondria. Propofol and vitamin E restored CCl(4)-induced changes in GSH, GPx, SOD and LPO in blood and liver mitochondria. CCl(4) decreased viability of mitochondria that was recovered by propofol and vitamin E. It is concluded that oxidative damage is the mechanism of toxicity of CCl(4) in the mitochondria that can be recovered by propofol comparable to vitamin E. Shaheed Beheshti University of Medical Sciences 2014 /pmc/articles/PMC3985254/ /pubmed/24734078 Text en © 2014 by School of Pharmacy, Shaheed Beheshti University of Medical Sciences and Health Services This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Ranjbar, Akram
Sharifzadeh, Mohammad
Karimi, Jamshid
Tavilani, Heidar
Baeeri, Maryam
Heidary shayesteh, Tavakol
Abdollahi, Mohammad
Propofol Attenuates Toxic Oxidative Stress by CCl(4) in Liver Mitochondria and Blood in Rat
title Propofol Attenuates Toxic Oxidative Stress by CCl(4) in Liver Mitochondria and Blood in Rat
title_full Propofol Attenuates Toxic Oxidative Stress by CCl(4) in Liver Mitochondria and Blood in Rat
title_fullStr Propofol Attenuates Toxic Oxidative Stress by CCl(4) in Liver Mitochondria and Blood in Rat
title_full_unstemmed Propofol Attenuates Toxic Oxidative Stress by CCl(4) in Liver Mitochondria and Blood in Rat
title_short Propofol Attenuates Toxic Oxidative Stress by CCl(4) in Liver Mitochondria and Blood in Rat
title_sort propofol attenuates toxic oxidative stress by ccl(4) in liver mitochondria and blood in rat
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3985254/
https://www.ncbi.nlm.nih.gov/pubmed/24734078
work_keys_str_mv AT ranjbarakram propofolattenuatestoxicoxidativestressbyccl4inlivermitochondriaandbloodinrat
AT sharifzadehmohammad propofolattenuatestoxicoxidativestressbyccl4inlivermitochondriaandbloodinrat
AT karimijamshid propofolattenuatestoxicoxidativestressbyccl4inlivermitochondriaandbloodinrat
AT tavilaniheidar propofolattenuatestoxicoxidativestressbyccl4inlivermitochondriaandbloodinrat
AT baeerimaryam propofolattenuatestoxicoxidativestressbyccl4inlivermitochondriaandbloodinrat
AT heidaryshayestehtavakol propofolattenuatestoxicoxidativestressbyccl4inlivermitochondriaandbloodinrat
AT abdollahimohammad propofolattenuatestoxicoxidativestressbyccl4inlivermitochondriaandbloodinrat