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Th9/IL-9 Profile in Human Echinococcosis: Their Involvement in Immune Response during Infection by Echinococcus granulosus
Th9 cells have been reported to contribute to immune responses; however, the role of Th9 cells in Echinococcus granulosus infection is unknown. This study is to determine whether Th9 cells and IL-9 are involved in human Echinococcus granulosus infection. Compared with healthy controls (HC group), th...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3985320/ https://www.ncbi.nlm.nih.gov/pubmed/24799769 http://dx.doi.org/10.1155/2014/781649 |
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author | Pang, Nannan Zhang, Fengbo Ma, Xiumin Zhang, Zhaoxia Zhao, Hui Xin, Yan Wang, Song Zhu, Yuejie Wen, Hao Ding, Jianbing |
author_facet | Pang, Nannan Zhang, Fengbo Ma, Xiumin Zhang, Zhaoxia Zhao, Hui Xin, Yan Wang, Song Zhu, Yuejie Wen, Hao Ding, Jianbing |
author_sort | Pang, Nannan |
collection | PubMed |
description | Th9 cells have been reported to contribute to immune responses; however, the role of Th9 cells in Echinococcus granulosus infection is unknown. This study is to determine whether Th9 cells and IL-9 are involved in human Echinococcus granulosus infection. Compared with healthy controls (HC group), the mRNA levels of PU.1, IL-9, and GATA-3 were significantly increased in patients before therapy (CE group), as revealed by qRT-PCR. Flow cytometry analysis showed that the percentages of Th9 and Th2 cells in CE group were significantly higher. The levels of IL-9, IL-4, IL-10, and TGF-β in CE group were also significantly increased, as detected by CBA assay. The percentages of Th9 and Th2 cells in CE group were positively correlated. After treatments of surgery in combination with albendazole, the PU.1 and GATA-3 mRNA levels were significantly decreased in patients after therapy (PCE group) compared with CE group. The numbers of Th9 and Th2 cells and levels of IL-9, IL-4, IL-10, and TGF-β were also significantly decreased in PCE group. In conclusion, the ratios of Th9 cells and IL-9 levels were significantly decreased after treatment, suggesting that Th9/IL-9 may be involved in immune response induced by Echinococcus granulosus infection. |
format | Online Article Text |
id | pubmed-3985320 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-39853202014-05-05 Th9/IL-9 Profile in Human Echinococcosis: Their Involvement in Immune Response during Infection by Echinococcus granulosus Pang, Nannan Zhang, Fengbo Ma, Xiumin Zhang, Zhaoxia Zhao, Hui Xin, Yan Wang, Song Zhu, Yuejie Wen, Hao Ding, Jianbing Mediators Inflamm Research Article Th9 cells have been reported to contribute to immune responses; however, the role of Th9 cells in Echinococcus granulosus infection is unknown. This study is to determine whether Th9 cells and IL-9 are involved in human Echinococcus granulosus infection. Compared with healthy controls (HC group), the mRNA levels of PU.1, IL-9, and GATA-3 were significantly increased in patients before therapy (CE group), as revealed by qRT-PCR. Flow cytometry analysis showed that the percentages of Th9 and Th2 cells in CE group were significantly higher. The levels of IL-9, IL-4, IL-10, and TGF-β in CE group were also significantly increased, as detected by CBA assay. The percentages of Th9 and Th2 cells in CE group were positively correlated. After treatments of surgery in combination with albendazole, the PU.1 and GATA-3 mRNA levels were significantly decreased in patients after therapy (PCE group) compared with CE group. The numbers of Th9 and Th2 cells and levels of IL-9, IL-4, IL-10, and TGF-β were also significantly decreased in PCE group. In conclusion, the ratios of Th9 cells and IL-9 levels were significantly decreased after treatment, suggesting that Th9/IL-9 may be involved in immune response induced by Echinococcus granulosus infection. Hindawi Publishing Corporation 2014 2014-03-30 /pmc/articles/PMC3985320/ /pubmed/24799769 http://dx.doi.org/10.1155/2014/781649 Text en Copyright © 2014 Nannan Pang et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Pang, Nannan Zhang, Fengbo Ma, Xiumin Zhang, Zhaoxia Zhao, Hui Xin, Yan Wang, Song Zhu, Yuejie Wen, Hao Ding, Jianbing Th9/IL-9 Profile in Human Echinococcosis: Their Involvement in Immune Response during Infection by Echinococcus granulosus |
title | Th9/IL-9 Profile in Human Echinococcosis: Their Involvement in Immune Response during Infection by Echinococcus granulosus
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title_full | Th9/IL-9 Profile in Human Echinococcosis: Their Involvement in Immune Response during Infection by Echinococcus granulosus
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title_fullStr | Th9/IL-9 Profile in Human Echinococcosis: Their Involvement in Immune Response during Infection by Echinococcus granulosus
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title_full_unstemmed | Th9/IL-9 Profile in Human Echinococcosis: Their Involvement in Immune Response during Infection by Echinococcus granulosus
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title_short | Th9/IL-9 Profile in Human Echinococcosis: Their Involvement in Immune Response during Infection by Echinococcus granulosus
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title_sort | th9/il-9 profile in human echinococcosis: their involvement in immune response during infection by echinococcus granulosus |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3985320/ https://www.ncbi.nlm.nih.gov/pubmed/24799769 http://dx.doi.org/10.1155/2014/781649 |
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