Cargando…

Th9/IL-9 Profile in Human Echinococcosis: Their Involvement in Immune Response during Infection by Echinococcus granulosus

Th9 cells have been reported to contribute to immune responses; however, the role of Th9 cells in Echinococcus granulosus infection is unknown. This study is to determine whether Th9 cells and IL-9 are involved in human Echinococcus granulosus infection. Compared with healthy controls (HC group), th...

Descripción completa

Detalles Bibliográficos
Autores principales: Pang, Nannan, Zhang, Fengbo, Ma, Xiumin, Zhang, Zhaoxia, Zhao, Hui, Xin, Yan, Wang, Song, Zhu, Yuejie, Wen, Hao, Ding, Jianbing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3985320/
https://www.ncbi.nlm.nih.gov/pubmed/24799769
http://dx.doi.org/10.1155/2014/781649
_version_ 1782311557256970240
author Pang, Nannan
Zhang, Fengbo
Ma, Xiumin
Zhang, Zhaoxia
Zhao, Hui
Xin, Yan
Wang, Song
Zhu, Yuejie
Wen, Hao
Ding, Jianbing
author_facet Pang, Nannan
Zhang, Fengbo
Ma, Xiumin
Zhang, Zhaoxia
Zhao, Hui
Xin, Yan
Wang, Song
Zhu, Yuejie
Wen, Hao
Ding, Jianbing
author_sort Pang, Nannan
collection PubMed
description Th9 cells have been reported to contribute to immune responses; however, the role of Th9 cells in Echinococcus granulosus infection is unknown. This study is to determine whether Th9 cells and IL-9 are involved in human Echinococcus granulosus infection. Compared with healthy controls (HC group), the mRNA levels of PU.1, IL-9, and GATA-3 were significantly increased in patients before therapy (CE group), as revealed by qRT-PCR. Flow cytometry analysis showed that the percentages of Th9 and Th2 cells in CE group were significantly higher. The levels of IL-9, IL-4, IL-10, and TGF-β in CE group were also significantly increased, as detected by CBA assay. The percentages of Th9 and Th2 cells in CE group were positively correlated. After treatments of surgery in combination with albendazole, the PU.1 and GATA-3 mRNA levels were significantly decreased in patients after therapy (PCE group) compared with CE group. The numbers of Th9 and Th2 cells and levels of IL-9, IL-4, IL-10, and TGF-β were also significantly decreased in PCE group. In conclusion, the ratios of Th9 cells and IL-9 levels were significantly decreased after treatment, suggesting that Th9/IL-9 may be involved in immune response induced by Echinococcus granulosus infection.
format Online
Article
Text
id pubmed-3985320
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-39853202014-05-05 Th9/IL-9 Profile in Human Echinococcosis: Their Involvement in Immune Response during Infection by Echinococcus granulosus Pang, Nannan Zhang, Fengbo Ma, Xiumin Zhang, Zhaoxia Zhao, Hui Xin, Yan Wang, Song Zhu, Yuejie Wen, Hao Ding, Jianbing Mediators Inflamm Research Article Th9 cells have been reported to contribute to immune responses; however, the role of Th9 cells in Echinococcus granulosus infection is unknown. This study is to determine whether Th9 cells and IL-9 are involved in human Echinococcus granulosus infection. Compared with healthy controls (HC group), the mRNA levels of PU.1, IL-9, and GATA-3 were significantly increased in patients before therapy (CE group), as revealed by qRT-PCR. Flow cytometry analysis showed that the percentages of Th9 and Th2 cells in CE group were significantly higher. The levels of IL-9, IL-4, IL-10, and TGF-β in CE group were also significantly increased, as detected by CBA assay. The percentages of Th9 and Th2 cells in CE group were positively correlated. After treatments of surgery in combination with albendazole, the PU.1 and GATA-3 mRNA levels were significantly decreased in patients after therapy (PCE group) compared with CE group. The numbers of Th9 and Th2 cells and levels of IL-9, IL-4, IL-10, and TGF-β were also significantly decreased in PCE group. In conclusion, the ratios of Th9 cells and IL-9 levels were significantly decreased after treatment, suggesting that Th9/IL-9 may be involved in immune response induced by Echinococcus granulosus infection. Hindawi Publishing Corporation 2014 2014-03-30 /pmc/articles/PMC3985320/ /pubmed/24799769 http://dx.doi.org/10.1155/2014/781649 Text en Copyright © 2014 Nannan Pang et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Pang, Nannan
Zhang, Fengbo
Ma, Xiumin
Zhang, Zhaoxia
Zhao, Hui
Xin, Yan
Wang, Song
Zhu, Yuejie
Wen, Hao
Ding, Jianbing
Th9/IL-9 Profile in Human Echinococcosis: Their Involvement in Immune Response during Infection by Echinococcus granulosus
title Th9/IL-9 Profile in Human Echinococcosis: Their Involvement in Immune Response during Infection by Echinococcus granulosus
title_full Th9/IL-9 Profile in Human Echinococcosis: Their Involvement in Immune Response during Infection by Echinococcus granulosus
title_fullStr Th9/IL-9 Profile in Human Echinococcosis: Their Involvement in Immune Response during Infection by Echinococcus granulosus
title_full_unstemmed Th9/IL-9 Profile in Human Echinococcosis: Their Involvement in Immune Response during Infection by Echinococcus granulosus
title_short Th9/IL-9 Profile in Human Echinococcosis: Their Involvement in Immune Response during Infection by Echinococcus granulosus
title_sort th9/il-9 profile in human echinococcosis: their involvement in immune response during infection by echinococcus granulosus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3985320/
https://www.ncbi.nlm.nih.gov/pubmed/24799769
http://dx.doi.org/10.1155/2014/781649
work_keys_str_mv AT pangnannan th9il9profileinhumanechinococcosistheirinvolvementinimmuneresponseduringinfectionbyechinococcusgranulosus
AT zhangfengbo th9il9profileinhumanechinococcosistheirinvolvementinimmuneresponseduringinfectionbyechinococcusgranulosus
AT maxiumin th9il9profileinhumanechinococcosistheirinvolvementinimmuneresponseduringinfectionbyechinococcusgranulosus
AT zhangzhaoxia th9il9profileinhumanechinococcosistheirinvolvementinimmuneresponseduringinfectionbyechinococcusgranulosus
AT zhaohui th9il9profileinhumanechinococcosistheirinvolvementinimmuneresponseduringinfectionbyechinococcusgranulosus
AT xinyan th9il9profileinhumanechinococcosistheirinvolvementinimmuneresponseduringinfectionbyechinococcusgranulosus
AT wangsong th9il9profileinhumanechinococcosistheirinvolvementinimmuneresponseduringinfectionbyechinococcusgranulosus
AT zhuyuejie th9il9profileinhumanechinococcosistheirinvolvementinimmuneresponseduringinfectionbyechinococcusgranulosus
AT wenhao th9il9profileinhumanechinococcosistheirinvolvementinimmuneresponseduringinfectionbyechinococcusgranulosus
AT dingjianbing th9il9profileinhumanechinococcosistheirinvolvementinimmuneresponseduringinfectionbyechinococcusgranulosus