Actin Controls the Vesicular Fraction of Dopamine Released During Extended Kiss and Run Exocytosis

[Image: see text] The effect of latrunculin A, an inhibitor of actin cross-linking, on exocytosis in PC12 cells was investigated with single cell amperometry. This analysis strongly suggests that the actin cytoskeleton might be involved in regulating exocytosis, especially by mediating the constrict...

Descripción completa

Detalles Bibliográficos
Autores principales: Trouillon, Raphaël, Ewing, Andrew G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3985473/
https://www.ncbi.nlm.nih.gov/pubmed/24400601
http://dx.doi.org/10.1021/cb400665f
_version_ 1782311578486439936
author Trouillon, Raphaël
Ewing, Andrew G.
author_facet Trouillon, Raphaël
Ewing, Andrew G.
author_sort Trouillon, Raphaël
collection PubMed
description [Image: see text] The effect of latrunculin A, an inhibitor of actin cross-linking, on exocytosis in PC12 cells was investigated with single cell amperometry. This analysis strongly suggests that the actin cytoskeleton might be involved in regulating exocytosis, especially by mediating the constriction of the pore. In an extended kiss-and-run release mode, actin could actually control the fraction of neurotransmitters released by the vesicle. This scaffold appears to contribute, with the lipid membrane and the protein machinery, to the closing dynamics of the pore, in competition with other forces mediating the opening of the exocytotic channel.
format Online
Article
Text
id pubmed-3985473
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-39854732015-01-08 Actin Controls the Vesicular Fraction of Dopamine Released During Extended Kiss and Run Exocytosis Trouillon, Raphaël Ewing, Andrew G. ACS Chem Biol [Image: see text] The effect of latrunculin A, an inhibitor of actin cross-linking, on exocytosis in PC12 cells was investigated with single cell amperometry. This analysis strongly suggests that the actin cytoskeleton might be involved in regulating exocytosis, especially by mediating the constriction of the pore. In an extended kiss-and-run release mode, actin could actually control the fraction of neurotransmitters released by the vesicle. This scaffold appears to contribute, with the lipid membrane and the protein machinery, to the closing dynamics of the pore, in competition with other forces mediating the opening of the exocytotic channel. American Chemical Society 2014-01-08 2014-03-21 /pmc/articles/PMC3985473/ /pubmed/24400601 http://dx.doi.org/10.1021/cb400665f Text en Copyright © 2014 American Chemical Society
spellingShingle Trouillon, Raphaël
Ewing, Andrew G.
Actin Controls the Vesicular Fraction of Dopamine Released During Extended Kiss and Run Exocytosis
title Actin Controls the Vesicular Fraction of Dopamine Released During Extended Kiss and Run Exocytosis
title_full Actin Controls the Vesicular Fraction of Dopamine Released During Extended Kiss and Run Exocytosis
title_fullStr Actin Controls the Vesicular Fraction of Dopamine Released During Extended Kiss and Run Exocytosis
title_full_unstemmed Actin Controls the Vesicular Fraction of Dopamine Released During Extended Kiss and Run Exocytosis
title_short Actin Controls the Vesicular Fraction of Dopamine Released During Extended Kiss and Run Exocytosis
title_sort actin controls the vesicular fraction of dopamine released during extended kiss and run exocytosis
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3985473/
https://www.ncbi.nlm.nih.gov/pubmed/24400601
http://dx.doi.org/10.1021/cb400665f
work_keys_str_mv AT trouillonraphael actincontrolsthevesicularfractionofdopaminereleasedduringextendedkissandrunexocytosis
AT ewingandrewg actincontrolsthevesicularfractionofdopaminereleasedduringextendedkissandrunexocytosis

Ejemplares similares