A Fast and Selective Near-Infrared Fluorescent Sensor for Multicolor Imaging of Biological Nitroxyl (HNO)

[Image: see text] The first near-infrared fluorescent turn-on sensor for the detection of nitroxyl (HNO), the one-electron reduced form of nitric oxide (NO), is reported. The new copper-based probe, CuDHX1, contains a dihydroxanthene (DHX) fluorophore and a cyclam derivative as a Cu(II) binding site...

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Autores principales: Wrobel, Alexandra T., Johnstone, Timothy C., Deliz Liang, Alexandria, Lippard, Stephen J., Rivera-Fuentes, Pablo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3985477/
https://www.ncbi.nlm.nih.gov/pubmed/24564324
http://dx.doi.org/10.1021/ja500315x
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author Wrobel, Alexandra T.
Johnstone, Timothy C.
Deliz Liang, Alexandria
Lippard, Stephen J.
Rivera-Fuentes, Pablo
author_facet Wrobel, Alexandra T.
Johnstone, Timothy C.
Deliz Liang, Alexandria
Lippard, Stephen J.
Rivera-Fuentes, Pablo
author_sort Wrobel, Alexandra T.
collection PubMed
description [Image: see text] The first near-infrared fluorescent turn-on sensor for the detection of nitroxyl (HNO), the one-electron reduced form of nitric oxide (NO), is reported. The new copper-based probe, CuDHX1, contains a dihydroxanthene (DHX) fluorophore and a cyclam derivative as a Cu(II) binding site. Upon reaction with HNO, CuDHX1 displays a five-fold fluorescence turn-on in cuvettes and is selective for HNO over thiols and reactive nitrogen and oxygen species. CuDHX1 can detect exogenously applied HNO in live mammalian cells and in conjunction with the zinc-specific, green-fluorescent sensor ZP1 can perform multicolor/multianalyte microscopic imaging. These studies reveal that HNO treatment elicits an increase in the concentration of intracellular mobile zinc.
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spelling pubmed-39854772015-02-24 A Fast and Selective Near-Infrared Fluorescent Sensor for Multicolor Imaging of Biological Nitroxyl (HNO) Wrobel, Alexandra T. Johnstone, Timothy C. Deliz Liang, Alexandria Lippard, Stephen J. Rivera-Fuentes, Pablo J Am Chem Soc [Image: see text] The first near-infrared fluorescent turn-on sensor for the detection of nitroxyl (HNO), the one-electron reduced form of nitric oxide (NO), is reported. The new copper-based probe, CuDHX1, contains a dihydroxanthene (DHX) fluorophore and a cyclam derivative as a Cu(II) binding site. Upon reaction with HNO, CuDHX1 displays a five-fold fluorescence turn-on in cuvettes and is selective for HNO over thiols and reactive nitrogen and oxygen species. CuDHX1 can detect exogenously applied HNO in live mammalian cells and in conjunction with the zinc-specific, green-fluorescent sensor ZP1 can perform multicolor/multianalyte microscopic imaging. These studies reveal that HNO treatment elicits an increase in the concentration of intracellular mobile zinc. American Chemical Society 2014-02-24 2014-03-26 /pmc/articles/PMC3985477/ /pubmed/24564324 http://dx.doi.org/10.1021/ja500315x Text en Copyright © 2014 American Chemical Society
spellingShingle Wrobel, Alexandra T.
Johnstone, Timothy C.
Deliz Liang, Alexandria
Lippard, Stephen J.
Rivera-Fuentes, Pablo
A Fast and Selective Near-Infrared Fluorescent Sensor for Multicolor Imaging of Biological Nitroxyl (HNO)
title A Fast and Selective Near-Infrared Fluorescent Sensor for Multicolor Imaging of Biological Nitroxyl (HNO)
title_full A Fast and Selective Near-Infrared Fluorescent Sensor for Multicolor Imaging of Biological Nitroxyl (HNO)
title_fullStr A Fast and Selective Near-Infrared Fluorescent Sensor for Multicolor Imaging of Biological Nitroxyl (HNO)
title_full_unstemmed A Fast and Selective Near-Infrared Fluorescent Sensor for Multicolor Imaging of Biological Nitroxyl (HNO)
title_short A Fast and Selective Near-Infrared Fluorescent Sensor for Multicolor Imaging of Biological Nitroxyl (HNO)
title_sort fast and selective near-infrared fluorescent sensor for multicolor imaging of biological nitroxyl (hno)
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3985477/
https://www.ncbi.nlm.nih.gov/pubmed/24564324
http://dx.doi.org/10.1021/ja500315x
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