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Burchellin: study of bioactivity against Aedes aegypti
BACKGROUND: The dengue mosquito Aedes aegypti Linnaeus, 1762 is a widespread insect pest of serious medical importance. Since no effective vaccine is available for treating dengue, the eradication or control of the main mosquito vector is regarded as essential. Since conventional insecticides have l...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3985536/ https://www.ncbi.nlm.nih.gov/pubmed/24713267 http://dx.doi.org/10.1186/1756-3305-7-172 |
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author | Narciso, Juliana Oliveira Abreu Soares, Renata Oliveira de Araújo Reis dos Santos Mallet, Jacenir Guimarães, Anthony Érico de Oliveira Chaves, Maria Célia Barbosa-Filho, José Maria Maleck, Marise |
author_facet | Narciso, Juliana Oliveira Abreu Soares, Renata Oliveira de Araújo Reis dos Santos Mallet, Jacenir Guimarães, Anthony Érico de Oliveira Chaves, Maria Célia Barbosa-Filho, José Maria Maleck, Marise |
author_sort | Narciso, Juliana Oliveira Abreu |
collection | PubMed |
description | BACKGROUND: The dengue mosquito Aedes aegypti Linnaeus, 1762 is a widespread insect pest of serious medical importance. Since no effective vaccine is available for treating dengue, the eradication or control of the main mosquito vector is regarded as essential. Since conventional insecticides have limited success, plants may be an alternative source of larvicidal agents, since they contain a rich source of bioactive chemicals. The aim of this study was to evaluate the larvicidal activity of the neolignan burchellin isolated from Ocotea cymbarum (Lauraceae), a plant from the Amazon region, against third instar larvae of A. aegypti. METHODS: Burchellin obtained from O. cymbarum was analyzed. The inhibitory activity against A. aegypti eggs and larvae and histological changes in the digestive system of treated L3 larvae were evaluated. In addition, nitric oxide synthase activity and nitric oxide levels were determined, and cytotoxicity bioassays performed. RESULTS: The data showed that burchellin interfered with the development cycle of the mosquito, where its strongest toxic effect was 100% mortality in larvae (L3) at concentrations ≥ 30 ppm. This compound did not show target cell toxicity in peritoneal macrophages from BALB/c mice, and proved to have molecular stability when dissolved in water. The L3 and L4 larvae treated with the compound showed cellular destruction and disorganization, cell spacing, and vacuolization of epithelial cells in small regions of the midgut. CONCLUSION: The neolignan burchellin proved to be a strong candidate for a natural, safe and stable phytolarvicidal to be used in population control of A. aegypti. |
format | Online Article Text |
id | pubmed-3985536 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-39855362014-04-15 Burchellin: study of bioactivity against Aedes aegypti Narciso, Juliana Oliveira Abreu Soares, Renata Oliveira de Araújo Reis dos Santos Mallet, Jacenir Guimarães, Anthony Érico de Oliveira Chaves, Maria Célia Barbosa-Filho, José Maria Maleck, Marise Parasit Vectors Research BACKGROUND: The dengue mosquito Aedes aegypti Linnaeus, 1762 is a widespread insect pest of serious medical importance. Since no effective vaccine is available for treating dengue, the eradication or control of the main mosquito vector is regarded as essential. Since conventional insecticides have limited success, plants may be an alternative source of larvicidal agents, since they contain a rich source of bioactive chemicals. The aim of this study was to evaluate the larvicidal activity of the neolignan burchellin isolated from Ocotea cymbarum (Lauraceae), a plant from the Amazon region, against third instar larvae of A. aegypti. METHODS: Burchellin obtained from O. cymbarum was analyzed. The inhibitory activity against A. aegypti eggs and larvae and histological changes in the digestive system of treated L3 larvae were evaluated. In addition, nitric oxide synthase activity and nitric oxide levels were determined, and cytotoxicity bioassays performed. RESULTS: The data showed that burchellin interfered with the development cycle of the mosquito, where its strongest toxic effect was 100% mortality in larvae (L3) at concentrations ≥ 30 ppm. This compound did not show target cell toxicity in peritoneal macrophages from BALB/c mice, and proved to have molecular stability when dissolved in water. The L3 and L4 larvae treated with the compound showed cellular destruction and disorganization, cell spacing, and vacuolization of epithelial cells in small regions of the midgut. CONCLUSION: The neolignan burchellin proved to be a strong candidate for a natural, safe and stable phytolarvicidal to be used in population control of A. aegypti. BioMed Central 2014-04-08 /pmc/articles/PMC3985536/ /pubmed/24713267 http://dx.doi.org/10.1186/1756-3305-7-172 Text en Copyright © 2014 Narciso et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. |
spellingShingle | Research Narciso, Juliana Oliveira Abreu Soares, Renata Oliveira de Araújo Reis dos Santos Mallet, Jacenir Guimarães, Anthony Érico de Oliveira Chaves, Maria Célia Barbosa-Filho, José Maria Maleck, Marise Burchellin: study of bioactivity against Aedes aegypti |
title | Burchellin: study of bioactivity against Aedes aegypti |
title_full | Burchellin: study of bioactivity against Aedes aegypti |
title_fullStr | Burchellin: study of bioactivity against Aedes aegypti |
title_full_unstemmed | Burchellin: study of bioactivity against Aedes aegypti |
title_short | Burchellin: study of bioactivity against Aedes aegypti |
title_sort | burchellin: study of bioactivity against aedes aegypti |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3985536/ https://www.ncbi.nlm.nih.gov/pubmed/24713267 http://dx.doi.org/10.1186/1756-3305-7-172 |
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