Rational optimization of tolC as a powerful dual selectable marker for genome engineering

Selection has been invaluable for genetic manipulation, although counter-selection has historically exhibited limited robustness and convenience. TolC, an outer membrane pore involved in transmembrane transport in E. coli, has been implemented as a selectable/counter-selectable marker, but counter-s...

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Detalles Bibliográficos
Autores principales: Gregg, Christopher J., Lajoie, Marc J., Napolitano, Michael G., Mosberg, Joshua A., Goodman, Daniel B., Aach, John, Isaacs, Farren J., Church, George M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
Synthetic Biology and Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3985617/
https://www.ncbi.nlm.nih.gov/pubmed/24452804
http://dx.doi.org/10.1093/nar/gkt1374

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3985617/
https://www.ncbi.nlm.nih.gov/pubmed/24452804
http://dx.doi.org/10.1093/nar/gkt1374

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