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Mini 12, Miniature Mass Spectrometer for Clinical and Other Applications—Introduction and Characterization
[Image: see text] A benchtop miniature mass spectrometer system, Mini 12, with ambient ionization source and tandem mass spectrometry capabilities has been developed and characterized. This instrument was developed as a self-contained system to produce quantitative results for unprocessed samples of...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American
Chemical
Society
2014
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3985695/ https://www.ncbi.nlm.nih.gov/pubmed/24521423 http://dx.doi.org/10.1021/ac403766c |
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author | Li, Linfan Chen, Tsung-Chi Ren, Yue Hendricks, Paul I. Cooks, R. Graham Ouyang, Zheng |
author_facet | Li, Linfan Chen, Tsung-Chi Ren, Yue Hendricks, Paul I. Cooks, R. Graham Ouyang, Zheng |
author_sort | Li, Linfan |
collection | PubMed |
description | [Image: see text] A benchtop miniature mass spectrometer system, Mini 12, with ambient ionization source and tandem mass spectrometry capabilities has been developed and characterized. This instrument was developed as a self-contained system to produce quantitative results for unprocessed samples of small volumes including nonvolatile analytes. The ion processing system, vacuum system, and control system are detailed. An integrated sample loading system facilitates automated operation. A user interface has been developed to acquire and to interpret analytical results for personnel who have limited mass spectrometry knowledge. Peak widths of Δm/z 0.6 Th (full width at half-maximum) and a mass range of up to m/z 900 are demonstrated with the rectilinear ion trap mass analyzer. Multistage experiments up to MS(5) are accomplished. Consumable cartridges have been designed for use in ambient paper spray ionization, and the recently developed extraction spray ionization method has been employed to improve the quantitative performance. Monitoring of trace-levels of chemicals in therapeutic drugs, as well as in food safety and environmental protection operations is demonstrated. Dual MS/MS scans are implemented to obtain the intensities of the fragment ions from the analyte and its internal standard, and the ratio is used in quantitative analysis of complex samples. Limits of quantitation (LOQ) of 7.5 ng/mL, with relative standard deviations below 10%, have been obtained for selected therapeutic drugs in whole blood throughout their individual therapeutic ranges. |
format | Online Article Text |
id | pubmed-3985695 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | American
Chemical
Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-39856952015-02-12 Mini 12, Miniature Mass Spectrometer for Clinical and Other Applications—Introduction and Characterization Li, Linfan Chen, Tsung-Chi Ren, Yue Hendricks, Paul I. Cooks, R. Graham Ouyang, Zheng Anal Chem [Image: see text] A benchtop miniature mass spectrometer system, Mini 12, with ambient ionization source and tandem mass spectrometry capabilities has been developed and characterized. This instrument was developed as a self-contained system to produce quantitative results for unprocessed samples of small volumes including nonvolatile analytes. The ion processing system, vacuum system, and control system are detailed. An integrated sample loading system facilitates automated operation. A user interface has been developed to acquire and to interpret analytical results for personnel who have limited mass spectrometry knowledge. Peak widths of Δm/z 0.6 Th (full width at half-maximum) and a mass range of up to m/z 900 are demonstrated with the rectilinear ion trap mass analyzer. Multistage experiments up to MS(5) are accomplished. Consumable cartridges have been designed for use in ambient paper spray ionization, and the recently developed extraction spray ionization method has been employed to improve the quantitative performance. Monitoring of trace-levels of chemicals in therapeutic drugs, as well as in food safety and environmental protection operations is demonstrated. Dual MS/MS scans are implemented to obtain the intensities of the fragment ions from the analyte and its internal standard, and the ratio is used in quantitative analysis of complex samples. Limits of quantitation (LOQ) of 7.5 ng/mL, with relative standard deviations below 10%, have been obtained for selected therapeutic drugs in whole blood throughout their individual therapeutic ranges. American Chemical Society 2014-02-12 2014-03-18 /pmc/articles/PMC3985695/ /pubmed/24521423 http://dx.doi.org/10.1021/ac403766c Text en Copyright © 2014 American Chemical Society |
spellingShingle | Li, Linfan Chen, Tsung-Chi Ren, Yue Hendricks, Paul I. Cooks, R. Graham Ouyang, Zheng Mini 12, Miniature Mass Spectrometer for Clinical and Other Applications—Introduction and Characterization |
title | Mini 12, Miniature Mass Spectrometer for Clinical
and Other Applications—Introduction and Characterization |
title_full | Mini 12, Miniature Mass Spectrometer for Clinical
and Other Applications—Introduction and Characterization |
title_fullStr | Mini 12, Miniature Mass Spectrometer for Clinical
and Other Applications—Introduction and Characterization |
title_full_unstemmed | Mini 12, Miniature Mass Spectrometer for Clinical
and Other Applications—Introduction and Characterization |
title_short | Mini 12, Miniature Mass Spectrometer for Clinical
and Other Applications—Introduction and Characterization |
title_sort | mini 12, miniature mass spectrometer for clinical
and other applications—introduction and characterization |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3985695/ https://www.ncbi.nlm.nih.gov/pubmed/24521423 http://dx.doi.org/10.1021/ac403766c |
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