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Mitochondrial Morphological Features Are Associated with Fission and Fusion Events

Mitochondria are dynamic organelles that undergo constant remodeling through the regulation of two opposing processes, mitochondrial fission and fusion. Although several key regulators and physiological stimuli have been identified to control mitochondrial fission and fusion, the role of mitochondri...

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Autores principales: Westrate, Laura M., Drocco, Jeffrey A., Martin, Katie R., Hlavacek, William S., MacKeigan, Jeffrey P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3986258/
https://www.ncbi.nlm.nih.gov/pubmed/24733410
http://dx.doi.org/10.1371/journal.pone.0095265
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author Westrate, Laura M.
Drocco, Jeffrey A.
Martin, Katie R.
Hlavacek, William S.
MacKeigan, Jeffrey P.
author_facet Westrate, Laura M.
Drocco, Jeffrey A.
Martin, Katie R.
Hlavacek, William S.
MacKeigan, Jeffrey P.
author_sort Westrate, Laura M.
collection PubMed
description Mitochondria are dynamic organelles that undergo constant remodeling through the regulation of two opposing processes, mitochondrial fission and fusion. Although several key regulators and physiological stimuli have been identified to control mitochondrial fission and fusion, the role of mitochondrial morphology in the two processes remains to be determined. To address this knowledge gap, we investigated whether morphological features extracted from time-lapse live-cell images of mitochondria could be used to predict mitochondrial fate. That is, we asked if we could predict whether a mitochondrion is likely to participate in a fission or fusion event based on its current shape and local environment. Using live-cell microscopy, image analysis software, and supervised machine learning, we characterized mitochondrial dynamics with single-organelle resolution to identify features of mitochondria that are predictive of fission and fusion events. A random forest (RF) model was trained to correctly classify mitochondria poised for either fission or fusion based on a series of morphological and positional features for each organelle. Of the features we evaluated, mitochondrial perimeter positively correlated with mitochondria about to undergo a fission event. Similarly mitochondrial solidity (compact shape) positively correlated with mitochondria about to undergo a fusion event. Our results indicate that fission and fusion are positively correlated with mitochondrial morphological features; and therefore, mitochondrial fission and fusion may be influenced by the mechanical properties of mitochondrial membranes.
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spelling pubmed-39862582014-04-15 Mitochondrial Morphological Features Are Associated with Fission and Fusion Events Westrate, Laura M. Drocco, Jeffrey A. Martin, Katie R. Hlavacek, William S. MacKeigan, Jeffrey P. PLoS One Research Article Mitochondria are dynamic organelles that undergo constant remodeling through the regulation of two opposing processes, mitochondrial fission and fusion. Although several key regulators and physiological stimuli have been identified to control mitochondrial fission and fusion, the role of mitochondrial morphology in the two processes remains to be determined. To address this knowledge gap, we investigated whether morphological features extracted from time-lapse live-cell images of mitochondria could be used to predict mitochondrial fate. That is, we asked if we could predict whether a mitochondrion is likely to participate in a fission or fusion event based on its current shape and local environment. Using live-cell microscopy, image analysis software, and supervised machine learning, we characterized mitochondrial dynamics with single-organelle resolution to identify features of mitochondria that are predictive of fission and fusion events. A random forest (RF) model was trained to correctly classify mitochondria poised for either fission or fusion based on a series of morphological and positional features for each organelle. Of the features we evaluated, mitochondrial perimeter positively correlated with mitochondria about to undergo a fission event. Similarly mitochondrial solidity (compact shape) positively correlated with mitochondria about to undergo a fusion event. Our results indicate that fission and fusion are positively correlated with mitochondrial morphological features; and therefore, mitochondrial fission and fusion may be influenced by the mechanical properties of mitochondrial membranes. Public Library of Science 2014-04-14 /pmc/articles/PMC3986258/ /pubmed/24733410 http://dx.doi.org/10.1371/journal.pone.0095265 Text en © 2014 Westrate et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Westrate, Laura M.
Drocco, Jeffrey A.
Martin, Katie R.
Hlavacek, William S.
MacKeigan, Jeffrey P.
Mitochondrial Morphological Features Are Associated with Fission and Fusion Events
title Mitochondrial Morphological Features Are Associated with Fission and Fusion Events
title_full Mitochondrial Morphological Features Are Associated with Fission and Fusion Events
title_fullStr Mitochondrial Morphological Features Are Associated with Fission and Fusion Events
title_full_unstemmed Mitochondrial Morphological Features Are Associated with Fission and Fusion Events
title_short Mitochondrial Morphological Features Are Associated with Fission and Fusion Events
title_sort mitochondrial morphological features are associated with fission and fusion events
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3986258/
https://www.ncbi.nlm.nih.gov/pubmed/24733410
http://dx.doi.org/10.1371/journal.pone.0095265
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