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Colonisation of a Phage Susceptible Campylobacter jejuni Population in Two Phage Positive Broiler Flocks

The pathogens Campylobacter jejuni and Campylobacter coli are commensals in the poultry intestine and campylobacteriosis is one of the most frequent foodborne diseases in developed and developing countries. Phages were identified to be effective in reducing intestinal Campylobacter load and this was...

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Autores principales: Kittler, Sophie, Fischer, Samuel, Abdulmawjood, Amir, Glünder, Gerhard, Klein, Günter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3986380/
https://www.ncbi.nlm.nih.gov/pubmed/24733154
http://dx.doi.org/10.1371/journal.pone.0094782
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author Kittler, Sophie
Fischer, Samuel
Abdulmawjood, Amir
Glünder, Gerhard
Klein, Günter
author_facet Kittler, Sophie
Fischer, Samuel
Abdulmawjood, Amir
Glünder, Gerhard
Klein, Günter
author_sort Kittler, Sophie
collection PubMed
description The pathogens Campylobacter jejuni and Campylobacter coli are commensals in the poultry intestine and campylobacteriosis is one of the most frequent foodborne diseases in developed and developing countries. Phages were identified to be effective in reducing intestinal Campylobacter load and this was evaluated, in the first field trials which were recently carried out. The aim of this study was to further investigate Campylobacter population dynamics during phage application on a commercial broiler farm. This study determines the superiority in colonisation of a Campylobacter type found in a field trial that was susceptible to phages in in vitro tests. The colonisation factors, i.e. motility and gamma glutamyl transferase activity, were increased in this type. The clustering in phylogenetic comparisons of MALDI-TOF spectra did not match the ST, biochemical phenotype and phage susceptibility. Occurrence of Campylobacter jejuni strains and phage susceptibility types with different colonisation potential seem to play a very important role in the success of phage therapy in commercial broiler houses. Thus, mechanisms of both, phage susceptibility and Campylobacter colonisation should be further investigated and considered when composing phage cocktails.
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spelling pubmed-39863802014-04-15 Colonisation of a Phage Susceptible Campylobacter jejuni Population in Two Phage Positive Broiler Flocks Kittler, Sophie Fischer, Samuel Abdulmawjood, Amir Glünder, Gerhard Klein, Günter PLoS One Research Article The pathogens Campylobacter jejuni and Campylobacter coli are commensals in the poultry intestine and campylobacteriosis is one of the most frequent foodborne diseases in developed and developing countries. Phages were identified to be effective in reducing intestinal Campylobacter load and this was evaluated, in the first field trials which were recently carried out. The aim of this study was to further investigate Campylobacter population dynamics during phage application on a commercial broiler farm. This study determines the superiority in colonisation of a Campylobacter type found in a field trial that was susceptible to phages in in vitro tests. The colonisation factors, i.e. motility and gamma glutamyl transferase activity, were increased in this type. The clustering in phylogenetic comparisons of MALDI-TOF spectra did not match the ST, biochemical phenotype and phage susceptibility. Occurrence of Campylobacter jejuni strains and phage susceptibility types with different colonisation potential seem to play a very important role in the success of phage therapy in commercial broiler houses. Thus, mechanisms of both, phage susceptibility and Campylobacter colonisation should be further investigated and considered when composing phage cocktails. Public Library of Science 2014-04-14 /pmc/articles/PMC3986380/ /pubmed/24733154 http://dx.doi.org/10.1371/journal.pone.0094782 Text en © 2014 Kittler et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kittler, Sophie
Fischer, Samuel
Abdulmawjood, Amir
Glünder, Gerhard
Klein, Günter
Colonisation of a Phage Susceptible Campylobacter jejuni Population in Two Phage Positive Broiler Flocks
title Colonisation of a Phage Susceptible Campylobacter jejuni Population in Two Phage Positive Broiler Flocks
title_full Colonisation of a Phage Susceptible Campylobacter jejuni Population in Two Phage Positive Broiler Flocks
title_fullStr Colonisation of a Phage Susceptible Campylobacter jejuni Population in Two Phage Positive Broiler Flocks
title_full_unstemmed Colonisation of a Phage Susceptible Campylobacter jejuni Population in Two Phage Positive Broiler Flocks
title_short Colonisation of a Phage Susceptible Campylobacter jejuni Population in Two Phage Positive Broiler Flocks
title_sort colonisation of a phage susceptible campylobacter jejuni population in two phage positive broiler flocks
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3986380/
https://www.ncbi.nlm.nih.gov/pubmed/24733154
http://dx.doi.org/10.1371/journal.pone.0094782
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