Cargando…
Radiolabelling of Antigen and Liposomes for Vaccine Biodistribution Studies
A relatively simple and effective method to follow the movement of pharmaceutical preparations such as vaccines in biodistribution studies is to radiolabel the components. Whilst single radiolabelling is common practice, in vaccine systems containing adjuvants the ability to follow both the adjuvant...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3986709/ https://www.ncbi.nlm.nih.gov/pubmed/27721345 http://dx.doi.org/10.3390/pharmaceutics2020091 |
_version_ | 1782311759436054528 |
---|---|
author | Henriksen-Lacey, Malou Bramwell, Vincent Perrie, Yvonne |
author_facet | Henriksen-Lacey, Malou Bramwell, Vincent Perrie, Yvonne |
author_sort | Henriksen-Lacey, Malou |
collection | PubMed |
description | A relatively simple and effective method to follow the movement of pharmaceutical preparations such as vaccines in biodistribution studies is to radiolabel the components. Whilst single radiolabelling is common practice, in vaccine systems containing adjuvants the ability to follow both the adjuvant and the antigen is favourable. To this end, we have devised a dual-radiolabelling method whereby the adjuvant (liposomes) is labelled with (3)H and the antigen (a subunit protein) with (125)I. This model is stable and reproducible; we have shown release of the radiolabels to be negligible over periods of up to 1 week in foetal calf serum at 37 °C. In this paper we describe the techniques which enable the radiolabelling of various components, assessing stability and processing of samples which all for their application in biodistribution studies. Furthermore we provide examples derived from our studies using this model in tuberculosis vaccine biodistribution studies. |
format | Online Article Text |
id | pubmed-3986709 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Molecular Diversity Preservation International |
record_format | MEDLINE/PubMed |
spelling | pubmed-39867092014-04-15 Radiolabelling of Antigen and Liposomes for Vaccine Biodistribution Studies Henriksen-Lacey, Malou Bramwell, Vincent Perrie, Yvonne Pharmaceutics Article A relatively simple and effective method to follow the movement of pharmaceutical preparations such as vaccines in biodistribution studies is to radiolabel the components. Whilst single radiolabelling is common practice, in vaccine systems containing adjuvants the ability to follow both the adjuvant and the antigen is favourable. To this end, we have devised a dual-radiolabelling method whereby the adjuvant (liposomes) is labelled with (3)H and the antigen (a subunit protein) with (125)I. This model is stable and reproducible; we have shown release of the radiolabels to be negligible over periods of up to 1 week in foetal calf serum at 37 °C. In this paper we describe the techniques which enable the radiolabelling of various components, assessing stability and processing of samples which all for their application in biodistribution studies. Furthermore we provide examples derived from our studies using this model in tuberculosis vaccine biodistribution studies. Molecular Diversity Preservation International 2010-03-31 /pmc/articles/PMC3986709/ /pubmed/27721345 http://dx.doi.org/10.3390/pharmaceutics2020091 Text en © 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Henriksen-Lacey, Malou Bramwell, Vincent Perrie, Yvonne Radiolabelling of Antigen and Liposomes for Vaccine Biodistribution Studies |
title | Radiolabelling of Antigen and Liposomes for Vaccine Biodistribution Studies |
title_full | Radiolabelling of Antigen and Liposomes for Vaccine Biodistribution Studies |
title_fullStr | Radiolabelling of Antigen and Liposomes for Vaccine Biodistribution Studies |
title_full_unstemmed | Radiolabelling of Antigen and Liposomes for Vaccine Biodistribution Studies |
title_short | Radiolabelling of Antigen and Liposomes for Vaccine Biodistribution Studies |
title_sort | radiolabelling of antigen and liposomes for vaccine biodistribution studies |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3986709/ https://www.ncbi.nlm.nih.gov/pubmed/27721345 http://dx.doi.org/10.3390/pharmaceutics2020091 |
work_keys_str_mv | AT henriksenlaceymalou radiolabellingofantigenandliposomesforvaccinebiodistributionstudies AT bramwellvincent radiolabellingofantigenandliposomesforvaccinebiodistributionstudies AT perrieyvonne radiolabellingofantigenandliposomesforvaccinebiodistributionstudies |