The Frequency of Mutations in Exon 11 of the c-kit Gene in Patients With Leukemia

Objective: To determine the frequency of mutations in exon 11 of the c-kit gene in patients with leukemia. Material and Methods: The study included 50 leukemia patients (31 with acute myeloid leukemia, 5 with acutelymphoblastic leukemia, 9 with chronic myeloid leukemia, and 5 with chronic lymphocyti...

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Autores principales: Hussain, Syed Rizwan, Babu, Sunil G, Naqvi, Hena, Singh, Pradyumn, Mahdi, Farzana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Galenos Publishing 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3986761/
https://www.ncbi.nlm.nih.gov/pubmed/24744618
http://dx.doi.org/10.5505/tjh.2012.60320
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author Hussain, Syed Rizwan
Babu, Sunil G
Naqvi, Hena
Singh, Pradyumn
Mahdi, Farzana
author_facet Hussain, Syed Rizwan
Babu, Sunil G
Naqvi, Hena
Singh, Pradyumn
Mahdi, Farzana
author_sort Hussain, Syed Rizwan
collection PubMed
description Objective: To determine the frequency of mutations in exon 11 of the c-kit gene in patients with leukemia. Material and Methods: The study included 50 leukemia patients (31 with acute myeloid leukemia, 5 with acutelymphoblastic leukemia, 9 with chronic myeloid leukemia, and 5 with chronic lymphocytic leukemia) that underwentPCR-SSCP, followed by direct DNA sequencing. Results: In all, 28 of the leukemia patients were male and 22 were female, with a mean age of 31.88 years (range: 2-65years). In total, 20 mutations in 19 patients were identified, including Lys550Asn, Tyr568Ser, Ile571Thr, Thr574Pro,Gln575His, Tyr578Pro, Asp579His, His580Gln, Arg586Thr, Asn587Asp, and Arg588Met, as well as novel point mutationsat codons Ile563Lys, Val569Leu, Tyr570Ser, and Pro577Ser. Ile571Leu substitution was observed in 2 patients andTrp582Ser substitution was observed in 3 patients. Conclusion: The results suggest that mutations in exon 11 of the c-kit gene might be useful as molecular geneticmarkers for leukemia
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spelling pubmed-39867612014-04-17 The Frequency of Mutations in Exon 11 of the c-kit Gene in Patients With Leukemia Hussain, Syed Rizwan Babu, Sunil G Naqvi, Hena Singh, Pradyumn Mahdi, Farzana Turk J Haematol Research Article Objective: To determine the frequency of mutations in exon 11 of the c-kit gene in patients with leukemia. Material and Methods: The study included 50 leukemia patients (31 with acute myeloid leukemia, 5 with acutelymphoblastic leukemia, 9 with chronic myeloid leukemia, and 5 with chronic lymphocytic leukemia) that underwentPCR-SSCP, followed by direct DNA sequencing. Results: In all, 28 of the leukemia patients were male and 22 were female, with a mean age of 31.88 years (range: 2-65years). In total, 20 mutations in 19 patients were identified, including Lys550Asn, Tyr568Ser, Ile571Thr, Thr574Pro,Gln575His, Tyr578Pro, Asp579His, His580Gln, Arg586Thr, Asn587Asp, and Arg588Met, as well as novel point mutationsat codons Ile563Lys, Val569Leu, Tyr570Ser, and Pro577Ser. Ile571Leu substitution was observed in 2 patients andTrp582Ser substitution was observed in 3 patients. Conclusion: The results suggest that mutations in exon 11 of the c-kit gene might be useful as molecular geneticmarkers for leukemia Galenos Publishing 2012-03 2012-03-15 /pmc/articles/PMC3986761/ /pubmed/24744618 http://dx.doi.org/10.5505/tjh.2012.60320 Text en © Turkish Journal of Hematology, Published by Galenos Publishing. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Hussain, Syed Rizwan
Babu, Sunil G
Naqvi, Hena
Singh, Pradyumn
Mahdi, Farzana
The Frequency of Mutations in Exon 11 of the c-kit Gene in Patients With Leukemia
title The Frequency of Mutations in Exon 11 of the c-kit Gene in Patients With Leukemia
title_full The Frequency of Mutations in Exon 11 of the c-kit Gene in Patients With Leukemia
title_fullStr The Frequency of Mutations in Exon 11 of the c-kit Gene in Patients With Leukemia
title_full_unstemmed The Frequency of Mutations in Exon 11 of the c-kit Gene in Patients With Leukemia
title_short The Frequency of Mutations in Exon 11 of the c-kit Gene in Patients With Leukemia
title_sort frequency of mutations in exon 11 of the c-kit gene in patients with leukemia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3986761/
https://www.ncbi.nlm.nih.gov/pubmed/24744618
http://dx.doi.org/10.5505/tjh.2012.60320
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