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Circadian oscillatory transcriptional programs in grapevine ripening fruits

BACKGROUND: Temperature and solar radiation influence Vitis vinifera L. berry ripening. Both environmental conditions fluctuate cyclically on a daily period basis and the strength of this fluctuation affects grape ripening too. Additionally, a molecular circadian clock regulates daily cyclic express...

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Autores principales: Carbonell-Bejerano, Pablo, Rodríguez, Virginia, Royo, Carolina, Hernáiz, Silvia, Moro-González, Luis Carlos, Torres-Viñals, Montserrat, Martínez-Zapater, José Miguel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3986946/
https://www.ncbi.nlm.nih.gov/pubmed/24666982
http://dx.doi.org/10.1186/1471-2229-14-78
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author Carbonell-Bejerano, Pablo
Rodríguez, Virginia
Royo, Carolina
Hernáiz, Silvia
Moro-González, Luis Carlos
Torres-Viñals, Montserrat
Martínez-Zapater, José Miguel
author_facet Carbonell-Bejerano, Pablo
Rodríguez, Virginia
Royo, Carolina
Hernáiz, Silvia
Moro-González, Luis Carlos
Torres-Viñals, Montserrat
Martínez-Zapater, José Miguel
author_sort Carbonell-Bejerano, Pablo
collection PubMed
description BACKGROUND: Temperature and solar radiation influence Vitis vinifera L. berry ripening. Both environmental conditions fluctuate cyclically on a daily period basis and the strength of this fluctuation affects grape ripening too. Additionally, a molecular circadian clock regulates daily cyclic expression in a large proportion of the plant transcriptome modulating multiple developmental processes in diverse plant organs and developmental phases. Circadian cycling of fruit transcriptomes has not been characterized in detail despite their putative relevance in the final composition of the fruit. Thus, in this study, gene expression throughout 24 h periods in pre-ripe berries of Tempranillo and Verdejo grapevine cultivars was followed to determine whether different ripening transcriptional programs are activated during certain times of day in different grape tissues and genotypes. RESULTS: Microarray analyses identified oscillatory transcriptional profiles following circadian variations in the photocycle and the thermocycle. A higher number of expression oscillating transcripts were detected in samples carrying exocarp tissue including biotic stress-responsive transcripts activated around dawn. Thermotolerance-like responses and regulation of circadian clock-related genes were observed in all studied samples. Indeed, homologs of core clock genes were identified in the grapevine genome and, among them, VvREVEILLE1 (VvRVE1), showed a consistent circadian expression rhythm in every grape berry tissue analysed. Light signalling components and terpenoid biosynthetic transcripts were specifically induced during the daytime in Verdejo, a cultivar bearing white-skinned and aromatic berries, whereas transcripts involved in phenylpropanoid biosynthesis were more prominently regulated in Tempranillo, a cultivar bearing black-skinned berries. CONCLUSIONS: The transcriptome of ripening fruits varies in response to daily environmental changes, which might partially be under the control of circadian clock components. Certain cultivar and berry tissue features could rely on specific circadian oscillatory expression profiles. These findings may help to a better understanding of the progress of berry ripening in short term time scales.
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spelling pubmed-39869462014-04-16 Circadian oscillatory transcriptional programs in grapevine ripening fruits Carbonell-Bejerano, Pablo Rodríguez, Virginia Royo, Carolina Hernáiz, Silvia Moro-González, Luis Carlos Torres-Viñals, Montserrat Martínez-Zapater, José Miguel BMC Plant Biol Research Article BACKGROUND: Temperature and solar radiation influence Vitis vinifera L. berry ripening. Both environmental conditions fluctuate cyclically on a daily period basis and the strength of this fluctuation affects grape ripening too. Additionally, a molecular circadian clock regulates daily cyclic expression in a large proportion of the plant transcriptome modulating multiple developmental processes in diverse plant organs and developmental phases. Circadian cycling of fruit transcriptomes has not been characterized in detail despite their putative relevance in the final composition of the fruit. Thus, in this study, gene expression throughout 24 h periods in pre-ripe berries of Tempranillo and Verdejo grapevine cultivars was followed to determine whether different ripening transcriptional programs are activated during certain times of day in different grape tissues and genotypes. RESULTS: Microarray analyses identified oscillatory transcriptional profiles following circadian variations in the photocycle and the thermocycle. A higher number of expression oscillating transcripts were detected in samples carrying exocarp tissue including biotic stress-responsive transcripts activated around dawn. Thermotolerance-like responses and regulation of circadian clock-related genes were observed in all studied samples. Indeed, homologs of core clock genes were identified in the grapevine genome and, among them, VvREVEILLE1 (VvRVE1), showed a consistent circadian expression rhythm in every grape berry tissue analysed. Light signalling components and terpenoid biosynthetic transcripts were specifically induced during the daytime in Verdejo, a cultivar bearing white-skinned and aromatic berries, whereas transcripts involved in phenylpropanoid biosynthesis were more prominently regulated in Tempranillo, a cultivar bearing black-skinned berries. CONCLUSIONS: The transcriptome of ripening fruits varies in response to daily environmental changes, which might partially be under the control of circadian clock components. Certain cultivar and berry tissue features could rely on specific circadian oscillatory expression profiles. These findings may help to a better understanding of the progress of berry ripening in short term time scales. BioMed Central 2014-03-25 /pmc/articles/PMC3986946/ /pubmed/24666982 http://dx.doi.org/10.1186/1471-2229-14-78 Text en Copyright © 2014 Carbonell-Bejerano et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Carbonell-Bejerano, Pablo
Rodríguez, Virginia
Royo, Carolina
Hernáiz, Silvia
Moro-González, Luis Carlos
Torres-Viñals, Montserrat
Martínez-Zapater, José Miguel
Circadian oscillatory transcriptional programs in grapevine ripening fruits
title Circadian oscillatory transcriptional programs in grapevine ripening fruits
title_full Circadian oscillatory transcriptional programs in grapevine ripening fruits
title_fullStr Circadian oscillatory transcriptional programs in grapevine ripening fruits
title_full_unstemmed Circadian oscillatory transcriptional programs in grapevine ripening fruits
title_short Circadian oscillatory transcriptional programs in grapevine ripening fruits
title_sort circadian oscillatory transcriptional programs in grapevine ripening fruits
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3986946/
https://www.ncbi.nlm.nih.gov/pubmed/24666982
http://dx.doi.org/10.1186/1471-2229-14-78
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