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Identification of two novel cytolysins from the hydrozoan Olindias sambaquiensis (Cnidaria)

BACKGROUND: Although the hydrozoan Olindias sambaquiensis is the most common jellyfish associated with human envenomation in southeastern and southern Brazil, information about the composition of its venom is rare. Thus, the present study aimed to analyze pharmacological aspects of O. sambaquiensis...

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Autores principales: Junior, Vidal Haddad, Zara, Fernando, Marangoni, Sergio, Toyama, Daniela de Oliveira, de Souza, Alex Jardelino Felizardo, de Oliveira, Simone Cristina Buzzo, Toyama, Marcos Hikari
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3987661/
https://www.ncbi.nlm.nih.gov/pubmed/24666608
http://dx.doi.org/10.1186/1678-9199-20-10
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author Junior, Vidal Haddad
Zara, Fernando
Marangoni, Sergio
Toyama, Daniela de Oliveira
de Souza, Alex Jardelino Felizardo
de Oliveira, Simone Cristina Buzzo
Toyama, Marcos Hikari
author_facet Junior, Vidal Haddad
Zara, Fernando
Marangoni, Sergio
Toyama, Daniela de Oliveira
de Souza, Alex Jardelino Felizardo
de Oliveira, Simone Cristina Buzzo
Toyama, Marcos Hikari
author_sort Junior, Vidal Haddad
collection PubMed
description BACKGROUND: Although the hydrozoan Olindias sambaquiensis is the most common jellyfish associated with human envenomation in southeastern and southern Brazil, information about the composition of its venom is rare. Thus, the present study aimed to analyze pharmacological aspects of O. sambaquiensis venom as well as clinical manifestations observed in affected patients. Crude protein extracts were prepared from the tentacles of animals; peptides and proteins were sequenced and submitted to circular dichroism spectroscopy. Creatine kinase, cytotoxicity and hemolytic activity were evaluated by specific methods. RESULTS: We identified two novel cytolysins denominated oshem 1 and oshem 2 from the tentacles of this jellyfish. The cytolysins presented the amino acid sequences NEGKAKCGNTAGSKLTFKSADECTKTGQK (oshem 1) and NNSKAKCGDLAGWSKLTFKSADECTKTGQKS (oshem 2) with respective molecular masses of 3.013 kDa and 3.375 kDa. Circular dichroism revealed that oshem 1 has random coils and small α-helix conformation as main secondary structure whereas oshem 2 presents mainly random coils as its main secondary structure probably due to the presence of W (13) in oshem 2. The hemolysis levels induced by oshem 1 and oshem 2 using a peptide concentration of 0.2 mg/mL were, respectively, 51.7 ± 6.5% and 32.9 ± 8.7% (n = 12 and p ≤ 0.05). Oshem 1 and oshem 2 showed significant myonecrotic activity, evaluated by respective CK level measurements of 1890.4 ± 89 and 1212.5 ± 103 (n = 4 and p ≤ 0.05). In addition, myonecrosis was also evaluated by cell survival, which was measured at 72.4 ± 8.6% and 83.5 ± 6.7% (n = 12 and p ≤ 0.05), respectively. The structural analysis showed that both oshem 1 and oshem 2 should be classified as a small basic hemolytic peptide. CONCLUSION: The amino acid sequences of two peptides were highly similar while the primary amino acid sequence analysis revealed W (22th) as the most important mutation. Finally oshem 1 and oshem 2 are the first cytolytic peptides isolated from the Olindias sambaquiensis and should probably represent a novel class of cytolytic peptides.
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spelling pubmed-39876612014-04-30 Identification of two novel cytolysins from the hydrozoan Olindias sambaquiensis (Cnidaria) Junior, Vidal Haddad Zara, Fernando Marangoni, Sergio Toyama, Daniela de Oliveira de Souza, Alex Jardelino Felizardo de Oliveira, Simone Cristina Buzzo Toyama, Marcos Hikari J Venom Anim Toxins Incl Trop Dis Research BACKGROUND: Although the hydrozoan Olindias sambaquiensis is the most common jellyfish associated with human envenomation in southeastern and southern Brazil, information about the composition of its venom is rare. Thus, the present study aimed to analyze pharmacological aspects of O. sambaquiensis venom as well as clinical manifestations observed in affected patients. Crude protein extracts were prepared from the tentacles of animals; peptides and proteins were sequenced and submitted to circular dichroism spectroscopy. Creatine kinase, cytotoxicity and hemolytic activity were evaluated by specific methods. RESULTS: We identified two novel cytolysins denominated oshem 1 and oshem 2 from the tentacles of this jellyfish. The cytolysins presented the amino acid sequences NEGKAKCGNTAGSKLTFKSADECTKTGQK (oshem 1) and NNSKAKCGDLAGWSKLTFKSADECTKTGQKS (oshem 2) with respective molecular masses of 3.013 kDa and 3.375 kDa. Circular dichroism revealed that oshem 1 has random coils and small α-helix conformation as main secondary structure whereas oshem 2 presents mainly random coils as its main secondary structure probably due to the presence of W (13) in oshem 2. The hemolysis levels induced by oshem 1 and oshem 2 using a peptide concentration of 0.2 mg/mL were, respectively, 51.7 ± 6.5% and 32.9 ± 8.7% (n = 12 and p ≤ 0.05). Oshem 1 and oshem 2 showed significant myonecrotic activity, evaluated by respective CK level measurements of 1890.4 ± 89 and 1212.5 ± 103 (n = 4 and p ≤ 0.05). In addition, myonecrosis was also evaluated by cell survival, which was measured at 72.4 ± 8.6% and 83.5 ± 6.7% (n = 12 and p ≤ 0.05), respectively. The structural analysis showed that both oshem 1 and oshem 2 should be classified as a small basic hemolytic peptide. CONCLUSION: The amino acid sequences of two peptides were highly similar while the primary amino acid sequence analysis revealed W (22th) as the most important mutation. Finally oshem 1 and oshem 2 are the first cytolytic peptides isolated from the Olindias sambaquiensis and should probably represent a novel class of cytolytic peptides. BioMed Central 2014-03-25 /pmc/articles/PMC3987661/ /pubmed/24666608 http://dx.doi.org/10.1186/1678-9199-20-10 Text en Copyright © 2014 Junior et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.
spellingShingle Research
Junior, Vidal Haddad
Zara, Fernando
Marangoni, Sergio
Toyama, Daniela de Oliveira
de Souza, Alex Jardelino Felizardo
de Oliveira, Simone Cristina Buzzo
Toyama, Marcos Hikari
Identification of two novel cytolysins from the hydrozoan Olindias sambaquiensis (Cnidaria)
title Identification of two novel cytolysins from the hydrozoan Olindias sambaquiensis (Cnidaria)
title_full Identification of two novel cytolysins from the hydrozoan Olindias sambaquiensis (Cnidaria)
title_fullStr Identification of two novel cytolysins from the hydrozoan Olindias sambaquiensis (Cnidaria)
title_full_unstemmed Identification of two novel cytolysins from the hydrozoan Olindias sambaquiensis (Cnidaria)
title_short Identification of two novel cytolysins from the hydrozoan Olindias sambaquiensis (Cnidaria)
title_sort identification of two novel cytolysins from the hydrozoan olindias sambaquiensis (cnidaria)
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3987661/
https://www.ncbi.nlm.nih.gov/pubmed/24666608
http://dx.doi.org/10.1186/1678-9199-20-10
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