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Cultivation Conditions for Phytase Production from Recombinant Escherichia coli DH5α

Response surface methodology (RSM) was used to optimize the cultivation conditions for the production of phytase by recombinant Escherichia coli DH5α. The optimum predicted cultivation conditions for phytase production were at 3 hours seed age, a 2.5% inoculum level, an L-arabinose concentration of...

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Autores principales: Ariff, Rafidah Mohd, Fitrianto, Anwar, Abd. Manap, Mohd Yazid, Ideris, Aini, Kassim, Azhar, Suhairin, Afinah, Hussin, Anis Shobirin Meor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Libertas Academica 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3987752/
https://www.ncbi.nlm.nih.gov/pubmed/24826071
http://dx.doi.org/10.4137/MBI.S10402
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author Ariff, Rafidah Mohd
Fitrianto, Anwar
Abd. Manap, Mohd Yazid
Ideris, Aini
Kassim, Azhar
Suhairin, Afinah
Hussin, Anis Shobirin Meor
author_facet Ariff, Rafidah Mohd
Fitrianto, Anwar
Abd. Manap, Mohd Yazid
Ideris, Aini
Kassim, Azhar
Suhairin, Afinah
Hussin, Anis Shobirin Meor
author_sort Ariff, Rafidah Mohd
collection PubMed
description Response surface methodology (RSM) was used to optimize the cultivation conditions for the production of phytase by recombinant Escherichia coli DH5α. The optimum predicted cultivation conditions for phytase production were at 3 hours seed age, a 2.5% inoculum level, an L-arabinose concentration of 0.20%, a cell concentration of 0.3 (as measured at 600 nm) and 17 hours post-induction time with a predicted phytase activity of 4194.45 U/mL. The model was validated and the results showed no significant difference between the experimental and the predicted phytase activity (P = 0.305). Under optimum cultivation conditions, the phytase activity of the recombinant E. coli DH5α was 364 times higher compared to the phytase activity of the wild-type producer, Enterobacter sakazakii ASUIA279. Hence, optimization of the cultivation conditions using RSM positively increased phytase production from recombinant E. coli DH5α.
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spelling pubmed-39877522014-05-13 Cultivation Conditions for Phytase Production from Recombinant Escherichia coli DH5α Ariff, Rafidah Mohd Fitrianto, Anwar Abd. Manap, Mohd Yazid Ideris, Aini Kassim, Azhar Suhairin, Afinah Hussin, Anis Shobirin Meor Microbiol Insights Original Research Response surface methodology (RSM) was used to optimize the cultivation conditions for the production of phytase by recombinant Escherichia coli DH5α. The optimum predicted cultivation conditions for phytase production were at 3 hours seed age, a 2.5% inoculum level, an L-arabinose concentration of 0.20%, a cell concentration of 0.3 (as measured at 600 nm) and 17 hours post-induction time with a predicted phytase activity of 4194.45 U/mL. The model was validated and the results showed no significant difference between the experimental and the predicted phytase activity (P = 0.305). Under optimum cultivation conditions, the phytase activity of the recombinant E. coli DH5α was 364 times higher compared to the phytase activity of the wild-type producer, Enterobacter sakazakii ASUIA279. Hence, optimization of the cultivation conditions using RSM positively increased phytase production from recombinant E. coli DH5α. Libertas Academica 2013-04-09 /pmc/articles/PMC3987752/ /pubmed/24826071 http://dx.doi.org/10.4137/MBI.S10402 Text en © 2013 the author(s), publisher and licensee Libertas Academica Ltd. This is an open access article. Unrestricted non-commercial use is permitted provided the original work is properly cited.
spellingShingle Original Research
Ariff, Rafidah Mohd
Fitrianto, Anwar
Abd. Manap, Mohd Yazid
Ideris, Aini
Kassim, Azhar
Suhairin, Afinah
Hussin, Anis Shobirin Meor
Cultivation Conditions for Phytase Production from Recombinant Escherichia coli DH5α
title Cultivation Conditions for Phytase Production from Recombinant Escherichia coli DH5α
title_full Cultivation Conditions for Phytase Production from Recombinant Escherichia coli DH5α
title_fullStr Cultivation Conditions for Phytase Production from Recombinant Escherichia coli DH5α
title_full_unstemmed Cultivation Conditions for Phytase Production from Recombinant Escherichia coli DH5α
title_short Cultivation Conditions for Phytase Production from Recombinant Escherichia coli DH5α
title_sort cultivation conditions for phytase production from recombinant escherichia coli dh5α
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3987752/
https://www.ncbi.nlm.nih.gov/pubmed/24826071
http://dx.doi.org/10.4137/MBI.S10402
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