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Long-Read Sequencing of Chicken Transcripts and Identification of New Transcript Isoforms

The chicken has long served as an important model organism in many fields, and continues to aid our understanding of animal development. Functional genomics studies aimed at probing the mechanisms that regulate development require high-quality genomes and transcript annotations. The quality of these...

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Detalles Bibliográficos
Autores principales: Thomas, Sean, Underwood, Jason G., Tseng, Elizabeth, Holloway, Alisha K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3988055/
https://www.ncbi.nlm.nih.gov/pubmed/24736250
http://dx.doi.org/10.1371/journal.pone.0094650
Descripción
Sumario:The chicken has long served as an important model organism in many fields, and continues to aid our understanding of animal development. Functional genomics studies aimed at probing the mechanisms that regulate development require high-quality genomes and transcript annotations. The quality of these resources has improved dramatically over the last several years, but many isoforms and genes have yet to be identified. We hope to contribute to the process of improving these resources with the data presented here: a set of long cDNA sequencing reads, and a curated set of new genes and transcript isoforms not currently represented in the most up-to-date genome annotation currently available to the community of researchers who rely on the chicken genome.