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Isolation and Characterization of Broad Spectrum Coaggregating Bacteria from Different Water Systems for Potential Use in Bioaugmentation
The bridging bacteria with broad-spectrum coaggregation ability play an important role during multispecies-biofilm development. In this study, through a visual and semi-quantitative assay, twenty-two bacterial strains with aggregation ability were obtained from 8 different water environments, and th...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3988075/ https://www.ncbi.nlm.nih.gov/pubmed/24736645 http://dx.doi.org/10.1371/journal.pone.0094220 |
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author | Cheng, Zhongqin Meng, Xiangxun Wang, Haichao Chen, Mei Li, Mengying |
author_facet | Cheng, Zhongqin Meng, Xiangxun Wang, Haichao Chen, Mei Li, Mengying |
author_sort | Cheng, Zhongqin |
collection | PubMed |
description | The bridging bacteria with broad-spectrum coaggregation ability play an important role during multispecies-biofilm development. In this study, through a visual and semi-quantitative assay, twenty-two bacterial strains with aggregation ability were obtained from 8 different water environments, and these strains were assigned to 7 genera according to their 16S rDNA and they were Aeromonas, Bacillus, Comamonas, Exiguobacterium, Pseudomonas, Shewanella and Comamonas. Furthermore, all possible 231 pairwise combinations among these 22 strains were explored for coaggregation ability by spectrophotometric assay. Among all these strains, it was found that Bacillus cereus G5 and Bacillus megaterium T1 coaggregated with themajority of assayed other strains, 90.5% (19 of 21 strains) and 76.2% respectively (17 of 21 strains) at a higher coaggregation rates (A.I. greater than 50%), indicating they have a broad-spectrum coaggregation property. The images of coaggregates also confirmed the coexistence of G5 and T1 with their partner strains. Biofilm biomass development of G5 cocultured with each of its partner strains were further evaluateded. The results showed that 15 of 21 strains, when paired with G5, developed greater biofilm biomass than the monocultures. Furthermore, the images from both fluorescence microscopy and scanning electron microscopy (SEM) demonstrated that G5 and A3-GFP (a 3,5-dinitrobenzoic acid-degrading strain, staining with gfp),could develop a typical spatial structure of dual-species biofilm when cocultured. These results suggested that bridging-bacteria with a broad spectrum coaggregating ability, such as G5,could mediate the integration of exogenous degrading bacteria into biofilms and contribute to the bioaugmentation treatment. |
format | Online Article Text |
id | pubmed-3988075 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-39880752014-04-21 Isolation and Characterization of Broad Spectrum Coaggregating Bacteria from Different Water Systems for Potential Use in Bioaugmentation Cheng, Zhongqin Meng, Xiangxun Wang, Haichao Chen, Mei Li, Mengying PLoS One Research Article The bridging bacteria with broad-spectrum coaggregation ability play an important role during multispecies-biofilm development. In this study, through a visual and semi-quantitative assay, twenty-two bacterial strains with aggregation ability were obtained from 8 different water environments, and these strains were assigned to 7 genera according to their 16S rDNA and they were Aeromonas, Bacillus, Comamonas, Exiguobacterium, Pseudomonas, Shewanella and Comamonas. Furthermore, all possible 231 pairwise combinations among these 22 strains were explored for coaggregation ability by spectrophotometric assay. Among all these strains, it was found that Bacillus cereus G5 and Bacillus megaterium T1 coaggregated with themajority of assayed other strains, 90.5% (19 of 21 strains) and 76.2% respectively (17 of 21 strains) at a higher coaggregation rates (A.I. greater than 50%), indicating they have a broad-spectrum coaggregation property. The images of coaggregates also confirmed the coexistence of G5 and T1 with their partner strains. Biofilm biomass development of G5 cocultured with each of its partner strains were further evaluateded. The results showed that 15 of 21 strains, when paired with G5, developed greater biofilm biomass than the monocultures. Furthermore, the images from both fluorescence microscopy and scanning electron microscopy (SEM) demonstrated that G5 and A3-GFP (a 3,5-dinitrobenzoic acid-degrading strain, staining with gfp),could develop a typical spatial structure of dual-species biofilm when cocultured. These results suggested that bridging-bacteria with a broad spectrum coaggregating ability, such as G5,could mediate the integration of exogenous degrading bacteria into biofilms and contribute to the bioaugmentation treatment. Public Library of Science 2014-04-15 /pmc/articles/PMC3988075/ /pubmed/24736645 http://dx.doi.org/10.1371/journal.pone.0094220 Text en © 2014 Cheng et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Cheng, Zhongqin Meng, Xiangxun Wang, Haichao Chen, Mei Li, Mengying Isolation and Characterization of Broad Spectrum Coaggregating Bacteria from Different Water Systems for Potential Use in Bioaugmentation |
title | Isolation and Characterization of Broad Spectrum Coaggregating Bacteria from Different Water Systems for Potential Use in Bioaugmentation |
title_full | Isolation and Characterization of Broad Spectrum Coaggregating Bacteria from Different Water Systems for Potential Use in Bioaugmentation |
title_fullStr | Isolation and Characterization of Broad Spectrum Coaggregating Bacteria from Different Water Systems for Potential Use in Bioaugmentation |
title_full_unstemmed | Isolation and Characterization of Broad Spectrum Coaggregating Bacteria from Different Water Systems for Potential Use in Bioaugmentation |
title_short | Isolation and Characterization of Broad Spectrum Coaggregating Bacteria from Different Water Systems for Potential Use in Bioaugmentation |
title_sort | isolation and characterization of broad spectrum coaggregating bacteria from different water systems for potential use in bioaugmentation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3988075/ https://www.ncbi.nlm.nih.gov/pubmed/24736645 http://dx.doi.org/10.1371/journal.pone.0094220 |
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