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Identification of the Immunodominant Regions of Staphylococcus aureus Fibronectin-Binding Protein A

Staphylococcus aureus is an opportunistic bacterial pathogen responsible for a diverse spectrum of human diseases and a leading cause of nosocomial and community-acquired infections. Development of a vaccine against this pathogen is an important goal. The fibronectin binding protein A (FnBPA) of S....

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Autores principales: Zuo, Qian-Fei, Cai, Chang-Zhi, Ding, Hong-Lei, Wu, Yi, Yang, Liu-Yang, Feng, Qiang, Yang, Hui-Jie, Wei, Zhen-Bo, Zeng, Hao, Zou, Quan-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3988184/
https://www.ncbi.nlm.nih.gov/pubmed/24736634
http://dx.doi.org/10.1371/journal.pone.0095338
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author Zuo, Qian-Fei
Cai, Chang-Zhi
Ding, Hong-Lei
Wu, Yi
Yang, Liu-Yang
Feng, Qiang
Yang, Hui-Jie
Wei, Zhen-Bo
Zeng, Hao
Zou, Quan-Ming
author_facet Zuo, Qian-Fei
Cai, Chang-Zhi
Ding, Hong-Lei
Wu, Yi
Yang, Liu-Yang
Feng, Qiang
Yang, Hui-Jie
Wei, Zhen-Bo
Zeng, Hao
Zou, Quan-Ming
author_sort Zuo, Qian-Fei
collection PubMed
description Staphylococcus aureus is an opportunistic bacterial pathogen responsible for a diverse spectrum of human diseases and a leading cause of nosocomial and community-acquired infections. Development of a vaccine against this pathogen is an important goal. The fibronectin binding protein A (FnBPA) of S. aureus is one of multifunctional ‘microbial surface components recognizing adhesive matrix molecules' (MSCRAMMs). It is one of the most important adhesin molecules involved in the initial adhesion steps of S. aureus infection. It has been studied as potential vaccine candidates. However, FnBPA is a high-molecular-weight protein of 106 kDa and difficulties in achieving its high-level expression in vitro limit its vaccine application in S. aureus infection diseases control. Therefore, mapping the immunodominant regions of FnBPA is important for developing polyvalent subunit fusion vaccines against S. aureus infections. In the present study, we cloned and expressed the N-terminal and C-terminal of FnBPA. We evaluated the immunogenicity of the two sections of FnBPA and the protective efficacy of the two truncated fragments vaccines in a murine model of systemic S. aureus infection. The results showed recombinant truncated fragment F1(30-500) had a strong immunogenicity property and survival rates significantly increased in the group of mice immunized with F1(30-500) than the control group. We futher identified the immunodominant regions of FnBPA. The mouse antisera reactions suggest that the region covering residues 110 to 263 (F1B(110-263)) is highly immunogenic and is the immunodominant regions of FnBPA. Moreover, vaccination with F1B(110-263) can generate partial protection against lethal challenge with two different S. aureus strains and reduced bacterial burdens against non-lethal challenge as well as that immunization with F1(30-500). This information will be important for further developing anti- S. aureus polyvalent subunit fusion vaccines.
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spelling pubmed-39881842014-04-21 Identification of the Immunodominant Regions of Staphylococcus aureus Fibronectin-Binding Protein A Zuo, Qian-Fei Cai, Chang-Zhi Ding, Hong-Lei Wu, Yi Yang, Liu-Yang Feng, Qiang Yang, Hui-Jie Wei, Zhen-Bo Zeng, Hao Zou, Quan-Ming PLoS One Research Article Staphylococcus aureus is an opportunistic bacterial pathogen responsible for a diverse spectrum of human diseases and a leading cause of nosocomial and community-acquired infections. Development of a vaccine against this pathogen is an important goal. The fibronectin binding protein A (FnBPA) of S. aureus is one of multifunctional ‘microbial surface components recognizing adhesive matrix molecules' (MSCRAMMs). It is one of the most important adhesin molecules involved in the initial adhesion steps of S. aureus infection. It has been studied as potential vaccine candidates. However, FnBPA is a high-molecular-weight protein of 106 kDa and difficulties in achieving its high-level expression in vitro limit its vaccine application in S. aureus infection diseases control. Therefore, mapping the immunodominant regions of FnBPA is important for developing polyvalent subunit fusion vaccines against S. aureus infections. In the present study, we cloned and expressed the N-terminal and C-terminal of FnBPA. We evaluated the immunogenicity of the two sections of FnBPA and the protective efficacy of the two truncated fragments vaccines in a murine model of systemic S. aureus infection. The results showed recombinant truncated fragment F1(30-500) had a strong immunogenicity property and survival rates significantly increased in the group of mice immunized with F1(30-500) than the control group. We futher identified the immunodominant regions of FnBPA. The mouse antisera reactions suggest that the region covering residues 110 to 263 (F1B(110-263)) is highly immunogenic and is the immunodominant regions of FnBPA. Moreover, vaccination with F1B(110-263) can generate partial protection against lethal challenge with two different S. aureus strains and reduced bacterial burdens against non-lethal challenge as well as that immunization with F1(30-500). This information will be important for further developing anti- S. aureus polyvalent subunit fusion vaccines. Public Library of Science 2014-04-15 /pmc/articles/PMC3988184/ /pubmed/24736634 http://dx.doi.org/10.1371/journal.pone.0095338 Text en © 2014 Zuo et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zuo, Qian-Fei
Cai, Chang-Zhi
Ding, Hong-Lei
Wu, Yi
Yang, Liu-Yang
Feng, Qiang
Yang, Hui-Jie
Wei, Zhen-Bo
Zeng, Hao
Zou, Quan-Ming
Identification of the Immunodominant Regions of Staphylococcus aureus Fibronectin-Binding Protein A
title Identification of the Immunodominant Regions of Staphylococcus aureus Fibronectin-Binding Protein A
title_full Identification of the Immunodominant Regions of Staphylococcus aureus Fibronectin-Binding Protein A
title_fullStr Identification of the Immunodominant Regions of Staphylococcus aureus Fibronectin-Binding Protein A
title_full_unstemmed Identification of the Immunodominant Regions of Staphylococcus aureus Fibronectin-Binding Protein A
title_short Identification of the Immunodominant Regions of Staphylococcus aureus Fibronectin-Binding Protein A
title_sort identification of the immunodominant regions of staphylococcus aureus fibronectin-binding protein a
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3988184/
https://www.ncbi.nlm.nih.gov/pubmed/24736634
http://dx.doi.org/10.1371/journal.pone.0095338
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