Cargando…
Identification of the Immunodominant Regions of Staphylococcus aureus Fibronectin-Binding Protein A
Staphylococcus aureus is an opportunistic bacterial pathogen responsible for a diverse spectrum of human diseases and a leading cause of nosocomial and community-acquired infections. Development of a vaccine against this pathogen is an important goal. The fibronectin binding protein A (FnBPA) of S....
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3988184/ https://www.ncbi.nlm.nih.gov/pubmed/24736634 http://dx.doi.org/10.1371/journal.pone.0095338 |
_version_ | 1782311996432056320 |
---|---|
author | Zuo, Qian-Fei Cai, Chang-Zhi Ding, Hong-Lei Wu, Yi Yang, Liu-Yang Feng, Qiang Yang, Hui-Jie Wei, Zhen-Bo Zeng, Hao Zou, Quan-Ming |
author_facet | Zuo, Qian-Fei Cai, Chang-Zhi Ding, Hong-Lei Wu, Yi Yang, Liu-Yang Feng, Qiang Yang, Hui-Jie Wei, Zhen-Bo Zeng, Hao Zou, Quan-Ming |
author_sort | Zuo, Qian-Fei |
collection | PubMed |
description | Staphylococcus aureus is an opportunistic bacterial pathogen responsible for a diverse spectrum of human diseases and a leading cause of nosocomial and community-acquired infections. Development of a vaccine against this pathogen is an important goal. The fibronectin binding protein A (FnBPA) of S. aureus is one of multifunctional ‘microbial surface components recognizing adhesive matrix molecules' (MSCRAMMs). It is one of the most important adhesin molecules involved in the initial adhesion steps of S. aureus infection. It has been studied as potential vaccine candidates. However, FnBPA is a high-molecular-weight protein of 106 kDa and difficulties in achieving its high-level expression in vitro limit its vaccine application in S. aureus infection diseases control. Therefore, mapping the immunodominant regions of FnBPA is important for developing polyvalent subunit fusion vaccines against S. aureus infections. In the present study, we cloned and expressed the N-terminal and C-terminal of FnBPA. We evaluated the immunogenicity of the two sections of FnBPA and the protective efficacy of the two truncated fragments vaccines in a murine model of systemic S. aureus infection. The results showed recombinant truncated fragment F1(30-500) had a strong immunogenicity property and survival rates significantly increased in the group of mice immunized with F1(30-500) than the control group. We futher identified the immunodominant regions of FnBPA. The mouse antisera reactions suggest that the region covering residues 110 to 263 (F1B(110-263)) is highly immunogenic and is the immunodominant regions of FnBPA. Moreover, vaccination with F1B(110-263) can generate partial protection against lethal challenge with two different S. aureus strains and reduced bacterial burdens against non-lethal challenge as well as that immunization with F1(30-500). This information will be important for further developing anti- S. aureus polyvalent subunit fusion vaccines. |
format | Online Article Text |
id | pubmed-3988184 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-39881842014-04-21 Identification of the Immunodominant Regions of Staphylococcus aureus Fibronectin-Binding Protein A Zuo, Qian-Fei Cai, Chang-Zhi Ding, Hong-Lei Wu, Yi Yang, Liu-Yang Feng, Qiang Yang, Hui-Jie Wei, Zhen-Bo Zeng, Hao Zou, Quan-Ming PLoS One Research Article Staphylococcus aureus is an opportunistic bacterial pathogen responsible for a diverse spectrum of human diseases and a leading cause of nosocomial and community-acquired infections. Development of a vaccine against this pathogen is an important goal. The fibronectin binding protein A (FnBPA) of S. aureus is one of multifunctional ‘microbial surface components recognizing adhesive matrix molecules' (MSCRAMMs). It is one of the most important adhesin molecules involved in the initial adhesion steps of S. aureus infection. It has been studied as potential vaccine candidates. However, FnBPA is a high-molecular-weight protein of 106 kDa and difficulties in achieving its high-level expression in vitro limit its vaccine application in S. aureus infection diseases control. Therefore, mapping the immunodominant regions of FnBPA is important for developing polyvalent subunit fusion vaccines against S. aureus infections. In the present study, we cloned and expressed the N-terminal and C-terminal of FnBPA. We evaluated the immunogenicity of the two sections of FnBPA and the protective efficacy of the two truncated fragments vaccines in a murine model of systemic S. aureus infection. The results showed recombinant truncated fragment F1(30-500) had a strong immunogenicity property and survival rates significantly increased in the group of mice immunized with F1(30-500) than the control group. We futher identified the immunodominant regions of FnBPA. The mouse antisera reactions suggest that the region covering residues 110 to 263 (F1B(110-263)) is highly immunogenic and is the immunodominant regions of FnBPA. Moreover, vaccination with F1B(110-263) can generate partial protection against lethal challenge with two different S. aureus strains and reduced bacterial burdens against non-lethal challenge as well as that immunization with F1(30-500). This information will be important for further developing anti- S. aureus polyvalent subunit fusion vaccines. Public Library of Science 2014-04-15 /pmc/articles/PMC3988184/ /pubmed/24736634 http://dx.doi.org/10.1371/journal.pone.0095338 Text en © 2014 Zuo et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Zuo, Qian-Fei Cai, Chang-Zhi Ding, Hong-Lei Wu, Yi Yang, Liu-Yang Feng, Qiang Yang, Hui-Jie Wei, Zhen-Bo Zeng, Hao Zou, Quan-Ming Identification of the Immunodominant Regions of Staphylococcus aureus Fibronectin-Binding Protein A |
title | Identification of the Immunodominant Regions of Staphylococcus aureus Fibronectin-Binding Protein A |
title_full | Identification of the Immunodominant Regions of Staphylococcus aureus Fibronectin-Binding Protein A |
title_fullStr | Identification of the Immunodominant Regions of Staphylococcus aureus Fibronectin-Binding Protein A |
title_full_unstemmed | Identification of the Immunodominant Regions of Staphylococcus aureus Fibronectin-Binding Protein A |
title_short | Identification of the Immunodominant Regions of Staphylococcus aureus Fibronectin-Binding Protein A |
title_sort | identification of the immunodominant regions of staphylococcus aureus fibronectin-binding protein a |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3988184/ https://www.ncbi.nlm.nih.gov/pubmed/24736634 http://dx.doi.org/10.1371/journal.pone.0095338 |
work_keys_str_mv | AT zuoqianfei identificationoftheimmunodominantregionsofstaphylococcusaureusfibronectinbindingproteina AT caichangzhi identificationoftheimmunodominantregionsofstaphylococcusaureusfibronectinbindingproteina AT dinghonglei identificationoftheimmunodominantregionsofstaphylococcusaureusfibronectinbindingproteina AT wuyi identificationoftheimmunodominantregionsofstaphylococcusaureusfibronectinbindingproteina AT yangliuyang identificationoftheimmunodominantregionsofstaphylococcusaureusfibronectinbindingproteina AT fengqiang identificationoftheimmunodominantregionsofstaphylococcusaureusfibronectinbindingproteina AT yanghuijie identificationoftheimmunodominantregionsofstaphylococcusaureusfibronectinbindingproteina AT weizhenbo identificationoftheimmunodominantregionsofstaphylococcusaureusfibronectinbindingproteina AT zenghao identificationoftheimmunodominantregionsofstaphylococcusaureusfibronectinbindingproteina AT zouquanming identificationoftheimmunodominantregionsofstaphylococcusaureusfibronectinbindingproteina |