Cargando…

The mutation of the rdxA gene in metronidazole-resistant Helicobacter pylori clinical isolates

BACKGROUNDS: Antibiotic resistance is an increasing problem throughout the developed world, and knowledge about different resistance mechanisms is consequential for efficient treatment of bacterial infections. Although metronidazole has been frequently used in treatment regimens for H. pylori infect...

Descripción completa

Detalles Bibliográficos
Autores principales: Mirzaei, Nasrin, Poursina, Farkhondeh, Moghim, Sharareh, Rahimi, Ebrahim, Safaei, Hajieh Ghasemian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3988589/
https://www.ncbi.nlm.nih.gov/pubmed/24761398
http://dx.doi.org/10.4103/2277-9175.128469
_version_ 1782312036787552256
author Mirzaei, Nasrin
Poursina, Farkhondeh
Moghim, Sharareh
Rahimi, Ebrahim
Safaei, Hajieh Ghasemian
author_facet Mirzaei, Nasrin
Poursina, Farkhondeh
Moghim, Sharareh
Rahimi, Ebrahim
Safaei, Hajieh Ghasemian
author_sort Mirzaei, Nasrin
collection PubMed
description BACKGROUNDS: Antibiotic resistance is an increasing problem throughout the developed world, and knowledge about different resistance mechanisms is consequential for efficient treatment of bacterial infections. Although metronidazole has been frequently used in treatment regimens for H. pylori infection, but antibiotic resistance is now a major contributing factor in treatment failure. Nevertheless metronidazole has been greatly used as a critical component of combination therapies for H. pylori infection. OBJECTIVE: This study is trying to describe the mutational mechanisms of metronidazole resistance in H. pylori in our clinical isolates in Isfahanian patients, Iran and compare with the findings of previous studies in world. MATERIALS AND METHODS: MIC values of metronidazole for H. pylori strains were determined by E- test. Both rdxA and glmM genes used for confirmation of isolates as H. pylori and then amplification of another rdxA oligonucleotide pair was done. Finally, the six resistant strains were sent to sequencing for other processing and further analysis was done by software. RESULTS: The result of six clinical isolates in comparison with 26695, J99 and 69A as a sensitive and resistant reference strains showed plenty of mutations. No frame shift and nonsense mutation was seen in our clinical isolates. CONCLUSION: An interesting finding in metronidazole-resistant strains in our study was the detection of one mutation not previously described in the literature in the rdxA gene and this W(209)R substitution presumably plays a role in inducing metronidazole resistance.
format Online
Article
Text
id pubmed-3988589
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Medknow Publications & Media Pvt Ltd
record_format MEDLINE/PubMed
spelling pubmed-39885892014-04-23 The mutation of the rdxA gene in metronidazole-resistant Helicobacter pylori clinical isolates Mirzaei, Nasrin Poursina, Farkhondeh Moghim, Sharareh Rahimi, Ebrahim Safaei, Hajieh Ghasemian Adv Biomed Res Original Article BACKGROUNDS: Antibiotic resistance is an increasing problem throughout the developed world, and knowledge about different resistance mechanisms is consequential for efficient treatment of bacterial infections. Although metronidazole has been frequently used in treatment regimens for H. pylori infection, but antibiotic resistance is now a major contributing factor in treatment failure. Nevertheless metronidazole has been greatly used as a critical component of combination therapies for H. pylori infection. OBJECTIVE: This study is trying to describe the mutational mechanisms of metronidazole resistance in H. pylori in our clinical isolates in Isfahanian patients, Iran and compare with the findings of previous studies in world. MATERIALS AND METHODS: MIC values of metronidazole for H. pylori strains were determined by E- test. Both rdxA and glmM genes used for confirmation of isolates as H. pylori and then amplification of another rdxA oligonucleotide pair was done. Finally, the six resistant strains were sent to sequencing for other processing and further analysis was done by software. RESULTS: The result of six clinical isolates in comparison with 26695, J99 and 69A as a sensitive and resistant reference strains showed plenty of mutations. No frame shift and nonsense mutation was seen in our clinical isolates. CONCLUSION: An interesting finding in metronidazole-resistant strains in our study was the detection of one mutation not previously described in the literature in the rdxA gene and this W(209)R substitution presumably plays a role in inducing metronidazole resistance. Medknow Publications & Media Pvt Ltd 2014-03-14 /pmc/articles/PMC3988589/ /pubmed/24761398 http://dx.doi.org/10.4103/2277-9175.128469 Text en Copyright: © 2014 Mirzaei. http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Original Article
Mirzaei, Nasrin
Poursina, Farkhondeh
Moghim, Sharareh
Rahimi, Ebrahim
Safaei, Hajieh Ghasemian
The mutation of the rdxA gene in metronidazole-resistant Helicobacter pylori clinical isolates
title The mutation of the rdxA gene in metronidazole-resistant Helicobacter pylori clinical isolates
title_full The mutation of the rdxA gene in metronidazole-resistant Helicobacter pylori clinical isolates
title_fullStr The mutation of the rdxA gene in metronidazole-resistant Helicobacter pylori clinical isolates
title_full_unstemmed The mutation of the rdxA gene in metronidazole-resistant Helicobacter pylori clinical isolates
title_short The mutation of the rdxA gene in metronidazole-resistant Helicobacter pylori clinical isolates
title_sort mutation of the rdxa gene in metronidazole-resistant helicobacter pylori clinical isolates
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3988589/
https://www.ncbi.nlm.nih.gov/pubmed/24761398
http://dx.doi.org/10.4103/2277-9175.128469
work_keys_str_mv AT mirzaeinasrin themutationoftherdxageneinmetronidazoleresistanthelicobacterpyloriclinicalisolates
AT poursinafarkhondeh themutationoftherdxageneinmetronidazoleresistanthelicobacterpyloriclinicalisolates
AT moghimsharareh themutationoftherdxageneinmetronidazoleresistanthelicobacterpyloriclinicalisolates
AT rahimiebrahim themutationoftherdxageneinmetronidazoleresistanthelicobacterpyloriclinicalisolates
AT safaeihajiehghasemian themutationoftherdxageneinmetronidazoleresistanthelicobacterpyloriclinicalisolates
AT mirzaeinasrin mutationoftherdxageneinmetronidazoleresistanthelicobacterpyloriclinicalisolates
AT poursinafarkhondeh mutationoftherdxageneinmetronidazoleresistanthelicobacterpyloriclinicalisolates
AT moghimsharareh mutationoftherdxageneinmetronidazoleresistanthelicobacterpyloriclinicalisolates
AT rahimiebrahim mutationoftherdxageneinmetronidazoleresistanthelicobacterpyloriclinicalisolates
AT safaeihajiehghasemian mutationoftherdxageneinmetronidazoleresistanthelicobacterpyloriclinicalisolates