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Analysis of Protein Interactions with Picomolar Binding Affinity by Fluorescence-Detected Sedimentation Velocity
[Image: see text] The study of high-affinity protein interactions with equilibrium dissociation constants (K(D)) in the picomolar range is of significant interest in many fields, but the characterization of stoichiometry and free energy of such high-affinity binding can be far from trivial. Analytic...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American
Chemical
Society
2014
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3988680/ https://www.ncbi.nlm.nih.gov/pubmed/24552356 http://dx.doi.org/10.1021/ac500093m |