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Efficacy of 1.5% Dish Washing Solution and 95% Lemon Water in Substituting Perilous Xylene as a Deparaffinizing Agent for Routine H and E Staining Procedure: A Short Study

Aim. To assess the efficacy of dish washing solution and diluted lemon water in deparaffinizing sections during conventional hematoxylin and eosin staining technique. Objective. The objective is to utilize eco-friendly economical substitute for xylene. Materials and Methods. Using twenty paraffin em...

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Autores principales: Ananthaneni, Anuradha, Namala, Srilekha, Guduru, Vijay Srinivas, Ramprasad, V. V. S., Ramisetty, Sabitha Devi, Udayashankar, Urmila, Naik, Kiran Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3988714/
https://www.ncbi.nlm.nih.gov/pubmed/24800109
http://dx.doi.org/10.1155/2014/707310
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author Ananthaneni, Anuradha
Namala, Srilekha
Guduru, Vijay Srinivas
Ramprasad, V. V. S.
Ramisetty, Sabitha Devi
Udayashankar, Urmila
Naik, Kiran Kumar
author_facet Ananthaneni, Anuradha
Namala, Srilekha
Guduru, Vijay Srinivas
Ramprasad, V. V. S.
Ramisetty, Sabitha Devi
Udayashankar, Urmila
Naik, Kiran Kumar
author_sort Ananthaneni, Anuradha
collection PubMed
description Aim. To assess the efficacy of dish washing solution and diluted lemon water in deparaffinizing sections during conventional hematoxylin and eosin staining technique. Objective. The objective is to utilize eco-friendly economical substitute for xylene. Materials and Methods. Using twenty paraffin embedded tissue blocks, three sections each were prepared. One section was stained with conventional H and E method (Group A) and the other two sections with xylene-free (XF) H and E (Groups B and C). Staining characteristics were compared with xylene and scoring was given. Total score of 3–5 was regarded as adequate for diagnosis and less than that inadequate for diagnosis. Statistical Analysis. Chi-square test, Kruskal Wallis ANOVA test, and Mann-Whitney U test were used. Results. Adequacy of nuclear staining, crispness, and staining for diagnosis were greater in both Groups A and C (100%) than Group B (95%). Adequacy of cytoplasmic staining was similar in all the three groups (100%). Group B showed comparatively superior uniform staining and less retention of wax. Conclusion. Dish washing solution or diluted lemon water can be replaced for xylene as deparaffinizing agent in hematoxylin and eosin procedure.
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spelling pubmed-39887142014-05-05 Efficacy of 1.5% Dish Washing Solution and 95% Lemon Water in Substituting Perilous Xylene as a Deparaffinizing Agent for Routine H and E Staining Procedure: A Short Study Ananthaneni, Anuradha Namala, Srilekha Guduru, Vijay Srinivas Ramprasad, V. V. S. Ramisetty, Sabitha Devi Udayashankar, Urmila Naik, Kiran Kumar Scientifica (Cairo) Research Article Aim. To assess the efficacy of dish washing solution and diluted lemon water in deparaffinizing sections during conventional hematoxylin and eosin staining technique. Objective. The objective is to utilize eco-friendly economical substitute for xylene. Materials and Methods. Using twenty paraffin embedded tissue blocks, three sections each were prepared. One section was stained with conventional H and E method (Group A) and the other two sections with xylene-free (XF) H and E (Groups B and C). Staining characteristics were compared with xylene and scoring was given. Total score of 3–5 was regarded as adequate for diagnosis and less than that inadequate for diagnosis. Statistical Analysis. Chi-square test, Kruskal Wallis ANOVA test, and Mann-Whitney U test were used. Results. Adequacy of nuclear staining, crispness, and staining for diagnosis were greater in both Groups A and C (100%) than Group B (95%). Adequacy of cytoplasmic staining was similar in all the three groups (100%). Group B showed comparatively superior uniform staining and less retention of wax. Conclusion. Dish washing solution or diluted lemon water can be replaced for xylene as deparaffinizing agent in hematoxylin and eosin procedure. Hindawi Publishing Corporation 2014 2014-03-31 /pmc/articles/PMC3988714/ /pubmed/24800109 http://dx.doi.org/10.1155/2014/707310 Text en Copyright © 2014 Anuradha Ananthaneni et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ananthaneni, Anuradha
Namala, Srilekha
Guduru, Vijay Srinivas
Ramprasad, V. V. S.
Ramisetty, Sabitha Devi
Udayashankar, Urmila
Naik, Kiran Kumar
Efficacy of 1.5% Dish Washing Solution and 95% Lemon Water in Substituting Perilous Xylene as a Deparaffinizing Agent for Routine H and E Staining Procedure: A Short Study
title Efficacy of 1.5% Dish Washing Solution and 95% Lemon Water in Substituting Perilous Xylene as a Deparaffinizing Agent for Routine H and E Staining Procedure: A Short Study
title_full Efficacy of 1.5% Dish Washing Solution and 95% Lemon Water in Substituting Perilous Xylene as a Deparaffinizing Agent for Routine H and E Staining Procedure: A Short Study
title_fullStr Efficacy of 1.5% Dish Washing Solution and 95% Lemon Water in Substituting Perilous Xylene as a Deparaffinizing Agent for Routine H and E Staining Procedure: A Short Study
title_full_unstemmed Efficacy of 1.5% Dish Washing Solution and 95% Lemon Water in Substituting Perilous Xylene as a Deparaffinizing Agent for Routine H and E Staining Procedure: A Short Study
title_short Efficacy of 1.5% Dish Washing Solution and 95% Lemon Water in Substituting Perilous Xylene as a Deparaffinizing Agent for Routine H and E Staining Procedure: A Short Study
title_sort efficacy of 1.5% dish washing solution and 95% lemon water in substituting perilous xylene as a deparaffinizing agent for routine h and e staining procedure: a short study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3988714/
https://www.ncbi.nlm.nih.gov/pubmed/24800109
http://dx.doi.org/10.1155/2014/707310
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