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Whole CMV Proteome Pattern Recognition Analysis after HSCT Identifies Unique Epitope Targets Associated with the CMV Status

Cytomegalovirus (CMV) infection represents a vital complication after Hematopoietic Stem Cell Transplantation (HSCT). We screened the entire CMV proteome to visualize the humoral target epitope-focus profile in serum after HSCT. IgG profiling from four patient groups (donor and/or recipient +/− for...

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Autores principales: Pérez-Bercoff, Lena, Valentini, Davide, Gaseitsiwe, Simani, Mahdavifar, Shahnaz, Schutkowski, Mike, Poiret, Thomas, Pérez-Bercoff, Åsa, Ljungman, Per, Maeurer, Markus J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3989190/
https://www.ncbi.nlm.nih.gov/pubmed/24740411
http://dx.doi.org/10.1371/journal.pone.0089648
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author Pérez-Bercoff, Lena
Valentini, Davide
Gaseitsiwe, Simani
Mahdavifar, Shahnaz
Schutkowski, Mike
Poiret, Thomas
Pérez-Bercoff, Åsa
Ljungman, Per
Maeurer, Markus J.
author_facet Pérez-Bercoff, Lena
Valentini, Davide
Gaseitsiwe, Simani
Mahdavifar, Shahnaz
Schutkowski, Mike
Poiret, Thomas
Pérez-Bercoff, Åsa
Ljungman, Per
Maeurer, Markus J.
author_sort Pérez-Bercoff, Lena
collection PubMed
description Cytomegalovirus (CMV) infection represents a vital complication after Hematopoietic Stem Cell Transplantation (HSCT). We screened the entire CMV proteome to visualize the humoral target epitope-focus profile in serum after HSCT. IgG profiling from four patient groups (donor and/or recipient +/− for CMV) was performed at 6, 12 and 24 months after HSCT using microarray slides containing 17174 of 15mer-peptides overlapping by 4 aa covering 214 proteins from CMV. Data were analyzed using maSigPro, PAM and the ‘exclusive recognition analysis (ERA)’ to identify unique CMV epitope responses for each patient group. The ‘exclusive recognition analysis’ of serum epitope patterns segregated best 12 months after HSCT for the D+/R+ group (versus D−/R−). Epitopes were derived from UL123 (IE1), UL99 (pp28), UL32 (pp150), this changed at 24 months to 2 strongly recognized peptides provided from UL123 and UL100. Strongly (IgG) recognized CMV targets elicited also robust cytokine production in T-cells from patients after HSCT defined by intracellular cytokine staining (IL-2, TNF, IFN and IL-17). High-content peptide microarrays allow epitope profiling of entire viral proteomes; this approach can be useful to map relevant targets for diagnostics and therapy in patients with well defined clinical endpoints. Peptide microarray analysis visualizes the breadth of B-cell immune reconstitution after HSCT and provides a useful tool to gauge immune reconstitution.
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spelling pubmed-39891902014-04-21 Whole CMV Proteome Pattern Recognition Analysis after HSCT Identifies Unique Epitope Targets Associated with the CMV Status Pérez-Bercoff, Lena Valentini, Davide Gaseitsiwe, Simani Mahdavifar, Shahnaz Schutkowski, Mike Poiret, Thomas Pérez-Bercoff, Åsa Ljungman, Per Maeurer, Markus J. PLoS One Research Article Cytomegalovirus (CMV) infection represents a vital complication after Hematopoietic Stem Cell Transplantation (HSCT). We screened the entire CMV proteome to visualize the humoral target epitope-focus profile in serum after HSCT. IgG profiling from four patient groups (donor and/or recipient +/− for CMV) was performed at 6, 12 and 24 months after HSCT using microarray slides containing 17174 of 15mer-peptides overlapping by 4 aa covering 214 proteins from CMV. Data were analyzed using maSigPro, PAM and the ‘exclusive recognition analysis (ERA)’ to identify unique CMV epitope responses for each patient group. The ‘exclusive recognition analysis’ of serum epitope patterns segregated best 12 months after HSCT for the D+/R+ group (versus D−/R−). Epitopes were derived from UL123 (IE1), UL99 (pp28), UL32 (pp150), this changed at 24 months to 2 strongly recognized peptides provided from UL123 and UL100. Strongly (IgG) recognized CMV targets elicited also robust cytokine production in T-cells from patients after HSCT defined by intracellular cytokine staining (IL-2, TNF, IFN and IL-17). High-content peptide microarrays allow epitope profiling of entire viral proteomes; this approach can be useful to map relevant targets for diagnostics and therapy in patients with well defined clinical endpoints. Peptide microarray analysis visualizes the breadth of B-cell immune reconstitution after HSCT and provides a useful tool to gauge immune reconstitution. Public Library of Science 2014-04-16 /pmc/articles/PMC3989190/ /pubmed/24740411 http://dx.doi.org/10.1371/journal.pone.0089648 Text en © 2014 Pérez-Bercoff et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Pérez-Bercoff, Lena
Valentini, Davide
Gaseitsiwe, Simani
Mahdavifar, Shahnaz
Schutkowski, Mike
Poiret, Thomas
Pérez-Bercoff, Åsa
Ljungman, Per
Maeurer, Markus J.
Whole CMV Proteome Pattern Recognition Analysis after HSCT Identifies Unique Epitope Targets Associated with the CMV Status
title Whole CMV Proteome Pattern Recognition Analysis after HSCT Identifies Unique Epitope Targets Associated with the CMV Status
title_full Whole CMV Proteome Pattern Recognition Analysis after HSCT Identifies Unique Epitope Targets Associated with the CMV Status
title_fullStr Whole CMV Proteome Pattern Recognition Analysis after HSCT Identifies Unique Epitope Targets Associated with the CMV Status
title_full_unstemmed Whole CMV Proteome Pattern Recognition Analysis after HSCT Identifies Unique Epitope Targets Associated with the CMV Status
title_short Whole CMV Proteome Pattern Recognition Analysis after HSCT Identifies Unique Epitope Targets Associated with the CMV Status
title_sort whole cmv proteome pattern recognition analysis after hsct identifies unique epitope targets associated with the cmv status
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3989190/
https://www.ncbi.nlm.nih.gov/pubmed/24740411
http://dx.doi.org/10.1371/journal.pone.0089648
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