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Dickkopf-Related Protein 1 Inhibits the WNT Signaling Pathway and Improves Pig Oocyte Maturation

The ability to mature oocytes in vitro provides a tool for creating embryos by parthenogenesis, fertilization, and cloning. Unfortunately the quality of oocytes matured in vitro falls behind that of in vivo matured oocytes. To address this difference, transcriptional profiling by deep sequencing was...

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Autores principales: Spate, Lee D., Brown, Alana N., Redel, Bethany K., Whitworth, Kristin M., Murphy, Clifton N., Prather, Randall S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3989281/
https://www.ncbi.nlm.nih.gov/pubmed/24739947
http://dx.doi.org/10.1371/journal.pone.0095114
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author Spate, Lee D.
Brown, Alana N.
Redel, Bethany K.
Whitworth, Kristin M.
Murphy, Clifton N.
Prather, Randall S.
author_facet Spate, Lee D.
Brown, Alana N.
Redel, Bethany K.
Whitworth, Kristin M.
Murphy, Clifton N.
Prather, Randall S.
author_sort Spate, Lee D.
collection PubMed
description The ability to mature oocytes in vitro provides a tool for creating embryos by parthenogenesis, fertilization, and cloning. Unfortunately the quality of oocytes matured in vitro falls behind that of in vivo matured oocytes. To address this difference, transcriptional profiling by deep sequencing was conducted on pig oocytes that were either matured in vitro or in vivo. Alignment of over 18 million reads identified 1,316 transcripts that were differentially represented. One pathway that was overrepresented in the oocytes matured in vitro was for Wingless-type MMTV integration site (WNT) signaling. In an attempt to inhibit the WNT pathway, Dickkopf-related protein 1 was added to the in vitro maturation medium. Addition of Dickkopf-related protein 1 improved the percentage of oocytes that matured to the metaphase II stage, increased the number of nuclei in the resulting blastocyst stage embryos, and reduced the amount of disheveled segment polarity protein 1 protein in oocytes. It is concluded that transcriptional profiling is a powerful method for detecting differences between in vitro and in vivo matured oocytes, and that the WNT signaling pathway is important for proper oocyte maturation.
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spelling pubmed-39892812014-04-21 Dickkopf-Related Protein 1 Inhibits the WNT Signaling Pathway and Improves Pig Oocyte Maturation Spate, Lee D. Brown, Alana N. Redel, Bethany K. Whitworth, Kristin M. Murphy, Clifton N. Prather, Randall S. PLoS One Research Article The ability to mature oocytes in vitro provides a tool for creating embryos by parthenogenesis, fertilization, and cloning. Unfortunately the quality of oocytes matured in vitro falls behind that of in vivo matured oocytes. To address this difference, transcriptional profiling by deep sequencing was conducted on pig oocytes that were either matured in vitro or in vivo. Alignment of over 18 million reads identified 1,316 transcripts that were differentially represented. One pathway that was overrepresented in the oocytes matured in vitro was for Wingless-type MMTV integration site (WNT) signaling. In an attempt to inhibit the WNT pathway, Dickkopf-related protein 1 was added to the in vitro maturation medium. Addition of Dickkopf-related protein 1 improved the percentage of oocytes that matured to the metaphase II stage, increased the number of nuclei in the resulting blastocyst stage embryos, and reduced the amount of disheveled segment polarity protein 1 protein in oocytes. It is concluded that transcriptional profiling is a powerful method for detecting differences between in vitro and in vivo matured oocytes, and that the WNT signaling pathway is important for proper oocyte maturation. Public Library of Science 2014-04-16 /pmc/articles/PMC3989281/ /pubmed/24739947 http://dx.doi.org/10.1371/journal.pone.0095114 Text en © 2014 Spate et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Spate, Lee D.
Brown, Alana N.
Redel, Bethany K.
Whitworth, Kristin M.
Murphy, Clifton N.
Prather, Randall S.
Dickkopf-Related Protein 1 Inhibits the WNT Signaling Pathway and Improves Pig Oocyte Maturation
title Dickkopf-Related Protein 1 Inhibits the WNT Signaling Pathway and Improves Pig Oocyte Maturation
title_full Dickkopf-Related Protein 1 Inhibits the WNT Signaling Pathway and Improves Pig Oocyte Maturation
title_fullStr Dickkopf-Related Protein 1 Inhibits the WNT Signaling Pathway and Improves Pig Oocyte Maturation
title_full_unstemmed Dickkopf-Related Protein 1 Inhibits the WNT Signaling Pathway and Improves Pig Oocyte Maturation
title_short Dickkopf-Related Protein 1 Inhibits the WNT Signaling Pathway and Improves Pig Oocyte Maturation
title_sort dickkopf-related protein 1 inhibits the wnt signaling pathway and improves pig oocyte maturation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3989281/
https://www.ncbi.nlm.nih.gov/pubmed/24739947
http://dx.doi.org/10.1371/journal.pone.0095114
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