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Strong Vaccine-Induced CD8 T-Cell Responses Have Cytolytic Function in a Chimpanzee Clearing HCV Infection

A single correlate of effective vaccine protection against chronic HCV infection has yet to be defined. In this study, we analyzed T-cell responses in four chimpanzees, immunized with core-E1-E2-NS3 and subsequently infected with HCV1b. Viral clearance was observed in one animal, while the other thr...

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Autores principales: Verstrepen, Babs E., Verschoor, Ernst J., Fagrouch, Zahra C., Mooij, Petra, de Groot, Natasja G., Bontrop, Ronald E., Bogers, Willy M., Heeney, Jonathan L., Koopman, Gerrit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3989318/
https://www.ncbi.nlm.nih.gov/pubmed/24740375
http://dx.doi.org/10.1371/journal.pone.0095103
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author Verstrepen, Babs E.
Verschoor, Ernst J.
Fagrouch, Zahra C.
Mooij, Petra
de Groot, Natasja G.
Bontrop, Ronald E.
Bogers, Willy M.
Heeney, Jonathan L.
Koopman, Gerrit
author_facet Verstrepen, Babs E.
Verschoor, Ernst J.
Fagrouch, Zahra C.
Mooij, Petra
de Groot, Natasja G.
Bontrop, Ronald E.
Bogers, Willy M.
Heeney, Jonathan L.
Koopman, Gerrit
author_sort Verstrepen, Babs E.
collection PubMed
description A single correlate of effective vaccine protection against chronic HCV infection has yet to be defined. In this study, we analyzed T-cell responses in four chimpanzees, immunized with core-E1-E2-NS3 and subsequently infected with HCV1b. Viral clearance was observed in one animal, while the other three became chronically infected. In the animal that cleared infection, NS3-specific CD8 T-cell responses were observed to be more potent in terms of frequency and polyfunctionality of cytokine producing cells. Unique to this animal was the presence of killing-competent CD8 T-cells, specific for NS3(1258–1272), being presented by the chimpanzee MHC class I molecule Patr-A*03∶01, and a high affinity recognition of this epitope. In the animals that became chronically infected, T-cells were able to produce cytokines against the same peptide but no cytolysis could be detected. In conclusion, in the animal that was able to clear HCV infection not only cytokine production was observed but also cytolytic potential against specific MHC class I/peptide-combinations.
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spelling pubmed-39893182014-04-21 Strong Vaccine-Induced CD8 T-Cell Responses Have Cytolytic Function in a Chimpanzee Clearing HCV Infection Verstrepen, Babs E. Verschoor, Ernst J. Fagrouch, Zahra C. Mooij, Petra de Groot, Natasja G. Bontrop, Ronald E. Bogers, Willy M. Heeney, Jonathan L. Koopman, Gerrit PLoS One Research Article A single correlate of effective vaccine protection against chronic HCV infection has yet to be defined. In this study, we analyzed T-cell responses in four chimpanzees, immunized with core-E1-E2-NS3 and subsequently infected with HCV1b. Viral clearance was observed in one animal, while the other three became chronically infected. In the animal that cleared infection, NS3-specific CD8 T-cell responses were observed to be more potent in terms of frequency and polyfunctionality of cytokine producing cells. Unique to this animal was the presence of killing-competent CD8 T-cells, specific for NS3(1258–1272), being presented by the chimpanzee MHC class I molecule Patr-A*03∶01, and a high affinity recognition of this epitope. In the animals that became chronically infected, T-cells were able to produce cytokines against the same peptide but no cytolysis could be detected. In conclusion, in the animal that was able to clear HCV infection not only cytokine production was observed but also cytolytic potential against specific MHC class I/peptide-combinations. Public Library of Science 2014-04-16 /pmc/articles/PMC3989318/ /pubmed/24740375 http://dx.doi.org/10.1371/journal.pone.0095103 Text en © 2014 Verstrepen et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Verstrepen, Babs E.
Verschoor, Ernst J.
Fagrouch, Zahra C.
Mooij, Petra
de Groot, Natasja G.
Bontrop, Ronald E.
Bogers, Willy M.
Heeney, Jonathan L.
Koopman, Gerrit
Strong Vaccine-Induced CD8 T-Cell Responses Have Cytolytic Function in a Chimpanzee Clearing HCV Infection
title Strong Vaccine-Induced CD8 T-Cell Responses Have Cytolytic Function in a Chimpanzee Clearing HCV Infection
title_full Strong Vaccine-Induced CD8 T-Cell Responses Have Cytolytic Function in a Chimpanzee Clearing HCV Infection
title_fullStr Strong Vaccine-Induced CD8 T-Cell Responses Have Cytolytic Function in a Chimpanzee Clearing HCV Infection
title_full_unstemmed Strong Vaccine-Induced CD8 T-Cell Responses Have Cytolytic Function in a Chimpanzee Clearing HCV Infection
title_short Strong Vaccine-Induced CD8 T-Cell Responses Have Cytolytic Function in a Chimpanzee Clearing HCV Infection
title_sort strong vaccine-induced cd8 t-cell responses have cytolytic function in a chimpanzee clearing hcv infection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3989318/
https://www.ncbi.nlm.nih.gov/pubmed/24740375
http://dx.doi.org/10.1371/journal.pone.0095103
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