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Foreign Body Reaction Associated with PET and PET/Chitosan Electrospun Nanofibrous Abdominal Meshes

Electrospun materials have been widely explored for biomedical applications because of their advantageous characteristics, i.e., tridimensional nanofibrous structure with high surface-to-volume ratio, high porosity, and pore interconnectivity. Furthermore, considering the similarities between the na...

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Autores principales: Veleirinho, Beatriz, Coelho, Daniela S., Dias, Paulo F., Maraschin, Marcelo, Pinto, Rúbia, Cargnin-Ferreira, Eduardo, Peixoto, Ana, Souza, José A., Ribeiro-do-Valle, Rosa M., Lopes-da-Silva, José A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3989343/
https://www.ncbi.nlm.nih.gov/pubmed/24740104
http://dx.doi.org/10.1371/journal.pone.0095293
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author Veleirinho, Beatriz
Coelho, Daniela S.
Dias, Paulo F.
Maraschin, Marcelo
Pinto, Rúbia
Cargnin-Ferreira, Eduardo
Peixoto, Ana
Souza, José A.
Ribeiro-do-Valle, Rosa M.
Lopes-da-Silva, José A.
author_facet Veleirinho, Beatriz
Coelho, Daniela S.
Dias, Paulo F.
Maraschin, Marcelo
Pinto, Rúbia
Cargnin-Ferreira, Eduardo
Peixoto, Ana
Souza, José A.
Ribeiro-do-Valle, Rosa M.
Lopes-da-Silva, José A.
author_sort Veleirinho, Beatriz
collection PubMed
description Electrospun materials have been widely explored for biomedical applications because of their advantageous characteristics, i.e., tridimensional nanofibrous structure with high surface-to-volume ratio, high porosity, and pore interconnectivity. Furthermore, considering the similarities between the nanofiber networks and the extracellular matrix (ECM), as well as the accepted role of changes in ECM for hernia repair, electrospun polymer fiber assemblies have emerged as potential materials for incisional hernia repair. In this work, we describe the application of electrospun non-absorbable mats based on poly(ethylene terephthalate) (PET) in the repair of abdominal defects, comparing the performance of these meshes with that of a commercial polypropylene mesh and a multifilament PET mesh. PET and PET/chitosan electrospun meshes revealed good performance during incisional hernia surgery, post-operative period, and no evidence of intestinal adhesion was found. The electrospun meshes were flexible with high suture retention, showing tensile strengths of 3 MPa and breaking strains of 8–33%. Nevertheless, a significant foreign body reaction (FBR) was observed in animals treated with the nanofibrous materials. Animals implanted with PET and PET/chitosan electrospun meshes (fiber diameter of 0.71±0.28 µm and 3.01±0.72 µm, respectively) showed, respectively, foreign body granuloma formation, averaging 4.2-fold and 7.4-fold greater than the control commercial mesh group (Marlex). Many foreign body giant cells (FBGC) involving nanofiber pieces were also found in the PET and PET/chitosan groups (11.9 and 19.3 times more FBGC than control, respectively). In contrast, no important FBR was observed for PET microfibers (fiber diameter = 18.9±0.21 µm). Therefore, we suggest that the reduced dimension and the high surface-to-volume ratio of the electrospun fibers caused the FBR reaction, pointing out the need for further studies to elucidate the mechanisms underlying interactions between cells/tissues and nanofibrous materials in order to gain a better understanding of the implantation risks associated with nanostructured biomaterials.
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spelling pubmed-39893432014-04-21 Foreign Body Reaction Associated with PET and PET/Chitosan Electrospun Nanofibrous Abdominal Meshes Veleirinho, Beatriz Coelho, Daniela S. Dias, Paulo F. Maraschin, Marcelo Pinto, Rúbia Cargnin-Ferreira, Eduardo Peixoto, Ana Souza, José A. Ribeiro-do-Valle, Rosa M. Lopes-da-Silva, José A. PLoS One Research Article Electrospun materials have been widely explored for biomedical applications because of their advantageous characteristics, i.e., tridimensional nanofibrous structure with high surface-to-volume ratio, high porosity, and pore interconnectivity. Furthermore, considering the similarities between the nanofiber networks and the extracellular matrix (ECM), as well as the accepted role of changes in ECM for hernia repair, electrospun polymer fiber assemblies have emerged as potential materials for incisional hernia repair. In this work, we describe the application of electrospun non-absorbable mats based on poly(ethylene terephthalate) (PET) in the repair of abdominal defects, comparing the performance of these meshes with that of a commercial polypropylene mesh and a multifilament PET mesh. PET and PET/chitosan electrospun meshes revealed good performance during incisional hernia surgery, post-operative period, and no evidence of intestinal adhesion was found. The electrospun meshes were flexible with high suture retention, showing tensile strengths of 3 MPa and breaking strains of 8–33%. Nevertheless, a significant foreign body reaction (FBR) was observed in animals treated with the nanofibrous materials. Animals implanted with PET and PET/chitosan electrospun meshes (fiber diameter of 0.71±0.28 µm and 3.01±0.72 µm, respectively) showed, respectively, foreign body granuloma formation, averaging 4.2-fold and 7.4-fold greater than the control commercial mesh group (Marlex). Many foreign body giant cells (FBGC) involving nanofiber pieces were also found in the PET and PET/chitosan groups (11.9 and 19.3 times more FBGC than control, respectively). In contrast, no important FBR was observed for PET microfibers (fiber diameter = 18.9±0.21 µm). Therefore, we suggest that the reduced dimension and the high surface-to-volume ratio of the electrospun fibers caused the FBR reaction, pointing out the need for further studies to elucidate the mechanisms underlying interactions between cells/tissues and nanofibrous materials in order to gain a better understanding of the implantation risks associated with nanostructured biomaterials. Public Library of Science 2014-04-16 /pmc/articles/PMC3989343/ /pubmed/24740104 http://dx.doi.org/10.1371/journal.pone.0095293 Text en © 2014 Veleirinho et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Veleirinho, Beatriz
Coelho, Daniela S.
Dias, Paulo F.
Maraschin, Marcelo
Pinto, Rúbia
Cargnin-Ferreira, Eduardo
Peixoto, Ana
Souza, José A.
Ribeiro-do-Valle, Rosa M.
Lopes-da-Silva, José A.
Foreign Body Reaction Associated with PET and PET/Chitosan Electrospun Nanofibrous Abdominal Meshes
title Foreign Body Reaction Associated with PET and PET/Chitosan Electrospun Nanofibrous Abdominal Meshes
title_full Foreign Body Reaction Associated with PET and PET/Chitosan Electrospun Nanofibrous Abdominal Meshes
title_fullStr Foreign Body Reaction Associated with PET and PET/Chitosan Electrospun Nanofibrous Abdominal Meshes
title_full_unstemmed Foreign Body Reaction Associated with PET and PET/Chitosan Electrospun Nanofibrous Abdominal Meshes
title_short Foreign Body Reaction Associated with PET and PET/Chitosan Electrospun Nanofibrous Abdominal Meshes
title_sort foreign body reaction associated with pet and pet/chitosan electrospun nanofibrous abdominal meshes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3989343/
https://www.ncbi.nlm.nih.gov/pubmed/24740104
http://dx.doi.org/10.1371/journal.pone.0095293
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