Design and Experimental Application of a Novel Non-Degenerate Universal Primer Set that Amplifies Prokaryotic 16S rRNA Genes with a Low Possibility to Amplify Eukaryotic rRNA Genes

The deep sequencing of 16S rRNA genes amplified by universal primers has revolutionized our understanding of microbial communities by allowing the characterization of the diversity of the uncultured majority. However, some universal primers also amplify eukaryotic rRNA genes, leading to a decrease i...

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Detalles Bibliográficos
Autores principales: Mori, Hiroshi, Maruyama, Fumito, Kato, Hiromi, Toyoda, Atsushi, Dozono, Ayumi, Ohtsubo, Yoshiyuki, Nagata, Yuji, Fujiyama, Asao, Tsuda, Masataka, Kurokawa, Ken
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
Full Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3989492/
https://www.ncbi.nlm.nih.gov/pubmed/24277737
http://dx.doi.org/10.1093/dnares/dst052

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