Cargando…
Integrating reductive and synthetic approaches in biology using man-made cell-like compartments
We propose ‘integrated synthetic genetics' as a novel methodology that integrates reductive and synthetic approaches used in life science research. Integrated synthetic genetics enables determinations of sets of genes required for the functioning of any biological subsystem. This method utilize...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3989557/ https://www.ncbi.nlm.nih.gov/pubmed/24740007 http://dx.doi.org/10.1038/srep04722 |
_version_ | 1782312166211190784 |
---|---|
author | Aoki, Wataru Saito, Masato Manabe, Ri-ichiroh Mori, Hirotada Yamaguchi, Yoshinori Tamiya, Eiichi |
author_facet | Aoki, Wataru Saito, Masato Manabe, Ri-ichiroh Mori, Hirotada Yamaguchi, Yoshinori Tamiya, Eiichi |
author_sort | Aoki, Wataru |
collection | PubMed |
description | We propose ‘integrated synthetic genetics' as a novel methodology that integrates reductive and synthetic approaches used in life science research. Integrated synthetic genetics enables determinations of sets of genes required for the functioning of any biological subsystem. This method utilizes artificial cell-like compartments, including a randomly introduced whole gene library, strictly defined components for in vitro transcription and translation and a reporter that fluoresces ‘only when a particular function of a target biological subsystem is active.' The set of genes necessary for the target biological subsystem can be identified by isolating fluorescent artificial cells and multiplex next-generation sequencing of genes included in these cells. The importance of this methodology is that screening for the set of genes involved in a subsystem and reconstructing the entire subsystem can be done simultaneously. This methodology can be applied to any biological subsystem of any species and may remarkably accelerate life science research. |
format | Online Article Text |
id | pubmed-3989557 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-39895572014-04-18 Integrating reductive and synthetic approaches in biology using man-made cell-like compartments Aoki, Wataru Saito, Masato Manabe, Ri-ichiroh Mori, Hirotada Yamaguchi, Yoshinori Tamiya, Eiichi Sci Rep Article We propose ‘integrated synthetic genetics' as a novel methodology that integrates reductive and synthetic approaches used in life science research. Integrated synthetic genetics enables determinations of sets of genes required for the functioning of any biological subsystem. This method utilizes artificial cell-like compartments, including a randomly introduced whole gene library, strictly defined components for in vitro transcription and translation and a reporter that fluoresces ‘only when a particular function of a target biological subsystem is active.' The set of genes necessary for the target biological subsystem can be identified by isolating fluorescent artificial cells and multiplex next-generation sequencing of genes included in these cells. The importance of this methodology is that screening for the set of genes involved in a subsystem and reconstructing the entire subsystem can be done simultaneously. This methodology can be applied to any biological subsystem of any species and may remarkably accelerate life science research. Nature Publishing Group 2014-04-17 /pmc/articles/PMC3989557/ /pubmed/24740007 http://dx.doi.org/10.1038/srep04722 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/3.0/ This work is licensed under a Creative Commons Attribution 3.0 Unported License. The images in this article are included in the article's Creative Commons license, unless indicated otherwise in the image credit; if the image is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the image. To view a copy of this license, visit http://creativecommons.org/licenses/by/3.0/ |
spellingShingle | Article Aoki, Wataru Saito, Masato Manabe, Ri-ichiroh Mori, Hirotada Yamaguchi, Yoshinori Tamiya, Eiichi Integrating reductive and synthetic approaches in biology using man-made cell-like compartments |
title | Integrating reductive and synthetic approaches in biology using man-made cell-like compartments |
title_full | Integrating reductive and synthetic approaches in biology using man-made cell-like compartments |
title_fullStr | Integrating reductive and synthetic approaches in biology using man-made cell-like compartments |
title_full_unstemmed | Integrating reductive and synthetic approaches in biology using man-made cell-like compartments |
title_short | Integrating reductive and synthetic approaches in biology using man-made cell-like compartments |
title_sort | integrating reductive and synthetic approaches in biology using man-made cell-like compartments |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3989557/ https://www.ncbi.nlm.nih.gov/pubmed/24740007 http://dx.doi.org/10.1038/srep04722 |
work_keys_str_mv | AT aokiwataru integratingreductiveandsyntheticapproachesinbiologyusingmanmadecelllikecompartments AT saitomasato integratingreductiveandsyntheticapproachesinbiologyusingmanmadecelllikecompartments AT manaberiichiroh integratingreductiveandsyntheticapproachesinbiologyusingmanmadecelllikecompartments AT morihirotada integratingreductiveandsyntheticapproachesinbiologyusingmanmadecelllikecompartments AT yamaguchiyoshinori integratingreductiveandsyntheticapproachesinbiologyusingmanmadecelllikecompartments AT tamiyaeiichi integratingreductiveandsyntheticapproachesinbiologyusingmanmadecelllikecompartments |