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Integrating reductive and synthetic approaches in biology using man-made cell-like compartments

We propose ‘integrated synthetic genetics' as a novel methodology that integrates reductive and synthetic approaches used in life science research. Integrated synthetic genetics enables determinations of sets of genes required for the functioning of any biological subsystem. This method utilize...

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Autores principales: Aoki, Wataru, Saito, Masato, Manabe, Ri-ichiroh, Mori, Hirotada, Yamaguchi, Yoshinori, Tamiya, Eiichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3989557/
https://www.ncbi.nlm.nih.gov/pubmed/24740007
http://dx.doi.org/10.1038/srep04722
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author Aoki, Wataru
Saito, Masato
Manabe, Ri-ichiroh
Mori, Hirotada
Yamaguchi, Yoshinori
Tamiya, Eiichi
author_facet Aoki, Wataru
Saito, Masato
Manabe, Ri-ichiroh
Mori, Hirotada
Yamaguchi, Yoshinori
Tamiya, Eiichi
author_sort Aoki, Wataru
collection PubMed
description We propose ‘integrated synthetic genetics' as a novel methodology that integrates reductive and synthetic approaches used in life science research. Integrated synthetic genetics enables determinations of sets of genes required for the functioning of any biological subsystem. This method utilizes artificial cell-like compartments, including a randomly introduced whole gene library, strictly defined components for in vitro transcription and translation and a reporter that fluoresces ‘only when a particular function of a target biological subsystem is active.' The set of genes necessary for the target biological subsystem can be identified by isolating fluorescent artificial cells and multiplex next-generation sequencing of genes included in these cells. The importance of this methodology is that screening for the set of genes involved in a subsystem and reconstructing the entire subsystem can be done simultaneously. This methodology can be applied to any biological subsystem of any species and may remarkably accelerate life science research.
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spelling pubmed-39895572014-04-18 Integrating reductive and synthetic approaches in biology using man-made cell-like compartments Aoki, Wataru Saito, Masato Manabe, Ri-ichiroh Mori, Hirotada Yamaguchi, Yoshinori Tamiya, Eiichi Sci Rep Article We propose ‘integrated synthetic genetics' as a novel methodology that integrates reductive and synthetic approaches used in life science research. Integrated synthetic genetics enables determinations of sets of genes required for the functioning of any biological subsystem. This method utilizes artificial cell-like compartments, including a randomly introduced whole gene library, strictly defined components for in vitro transcription and translation and a reporter that fluoresces ‘only when a particular function of a target biological subsystem is active.' The set of genes necessary for the target biological subsystem can be identified by isolating fluorescent artificial cells and multiplex next-generation sequencing of genes included in these cells. The importance of this methodology is that screening for the set of genes involved in a subsystem and reconstructing the entire subsystem can be done simultaneously. This methodology can be applied to any biological subsystem of any species and may remarkably accelerate life science research. Nature Publishing Group 2014-04-17 /pmc/articles/PMC3989557/ /pubmed/24740007 http://dx.doi.org/10.1038/srep04722 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/3.0/ This work is licensed under a Creative Commons Attribution 3.0 Unported License. The images in this article are included in the article's Creative Commons license, unless indicated otherwise in the image credit; if the image is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the image. To view a copy of this license, visit http://creativecommons.org/licenses/by/3.0/
spellingShingle Article
Aoki, Wataru
Saito, Masato
Manabe, Ri-ichiroh
Mori, Hirotada
Yamaguchi, Yoshinori
Tamiya, Eiichi
Integrating reductive and synthetic approaches in biology using man-made cell-like compartments
title Integrating reductive and synthetic approaches in biology using man-made cell-like compartments
title_full Integrating reductive and synthetic approaches in biology using man-made cell-like compartments
title_fullStr Integrating reductive and synthetic approaches in biology using man-made cell-like compartments
title_full_unstemmed Integrating reductive and synthetic approaches in biology using man-made cell-like compartments
title_short Integrating reductive and synthetic approaches in biology using man-made cell-like compartments
title_sort integrating reductive and synthetic approaches in biology using man-made cell-like compartments
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3989557/
https://www.ncbi.nlm.nih.gov/pubmed/24740007
http://dx.doi.org/10.1038/srep04722
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