CRISPR/Cas9 Allows Efficient and Complete Knock-In of a Destabilization Domain-Tagged Essential Protein in a Human Cell Line, Allowing Rapid Knockdown of Protein Function

Although modulation of protein levels is an important tool for study of protein function, it is difficult or impossible to knockdown or knockout genes that are critical for cell growth or viability. For such genes, a conditional knockdown approach would be valuable. The FKBP protein-based destabiliz...

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Detalles Bibliográficos
Autores principales: Park, Arnold, Won, Sohui T., Pentecost, Mickey, Bartkowski, Wojciech, Lee, Benhur
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3990584/
https://www.ncbi.nlm.nih.gov/pubmed/24743236
http://dx.doi.org/10.1371/journal.pone.0095101

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3990584/
https://www.ncbi.nlm.nih.gov/pubmed/24743236
http://dx.doi.org/10.1371/journal.pone.0095101

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