Mucosal Adjuvant Activity of IL-2 Presenting Spores of Bacillus subtilis in a Murine Model of Helicobacter pylori Vaccination

The endospores of Bacillus subtilis are now widely used as a platform for presentation of heterologous proteins and due to their safety record and high resistance to harsh environmental conditions can be considered as potential vehicles for oral vaccination. In this research we show that recombinant...

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Autores principales: Hinc, Krzysztof, Stasiłojć, Małgorzata, Piątek, Iwona, Peszyńska-Sularz, Grażyna, Isticato, Rachele, Ricca, Ezio, Obuchowski, Michał, Iwanicki, Adam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3990602/
https://www.ncbi.nlm.nih.gov/pubmed/24743850
http://dx.doi.org/10.1371/journal.pone.0095187
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author Hinc, Krzysztof
Stasiłojć, Małgorzata
Piątek, Iwona
Peszyńska-Sularz, Grażyna
Isticato, Rachele
Ricca, Ezio
Obuchowski, Michał
Iwanicki, Adam
author_facet Hinc, Krzysztof
Stasiłojć, Małgorzata
Piątek, Iwona
Peszyńska-Sularz, Grażyna
Isticato, Rachele
Ricca, Ezio
Obuchowski, Michał
Iwanicki, Adam
author_sort Hinc, Krzysztof
collection PubMed
description The endospores of Bacillus subtilis are now widely used as a platform for presentation of heterologous proteins and due to their safety record and high resistance to harsh environmental conditions can be considered as potential vehicles for oral vaccination. In this research we show that recombinant B. subtilis spores presenting a fragment of the Helicobacter acinonychis UreB protein and expressing the ureB gene under vegetative promoter elicit a strong cellular immune response in orally immunized mice when co-administered with spores presenting IL-2. We show for the first time the successful application of two types of recombinant spores, one carrying an antigen and the other an adjuvant, in a single oral immunization.
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spelling pubmed-39906022014-04-21 Mucosal Adjuvant Activity of IL-2 Presenting Spores of Bacillus subtilis in a Murine Model of Helicobacter pylori Vaccination Hinc, Krzysztof Stasiłojć, Małgorzata Piątek, Iwona Peszyńska-Sularz, Grażyna Isticato, Rachele Ricca, Ezio Obuchowski, Michał Iwanicki, Adam PLoS One Research Article The endospores of Bacillus subtilis are now widely used as a platform for presentation of heterologous proteins and due to their safety record and high resistance to harsh environmental conditions can be considered as potential vehicles for oral vaccination. In this research we show that recombinant B. subtilis spores presenting a fragment of the Helicobacter acinonychis UreB protein and expressing the ureB gene under vegetative promoter elicit a strong cellular immune response in orally immunized mice when co-administered with spores presenting IL-2. We show for the first time the successful application of two types of recombinant spores, one carrying an antigen and the other an adjuvant, in a single oral immunization. Public Library of Science 2014-04-17 /pmc/articles/PMC3990602/ /pubmed/24743850 http://dx.doi.org/10.1371/journal.pone.0095187 Text en © 2014 Hinc et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Hinc, Krzysztof
Stasiłojć, Małgorzata
Piątek, Iwona
Peszyńska-Sularz, Grażyna
Isticato, Rachele
Ricca, Ezio
Obuchowski, Michał
Iwanicki, Adam
Mucosal Adjuvant Activity of IL-2 Presenting Spores of Bacillus subtilis in a Murine Model of Helicobacter pylori Vaccination
title Mucosal Adjuvant Activity of IL-2 Presenting Spores of Bacillus subtilis in a Murine Model of Helicobacter pylori Vaccination
title_full Mucosal Adjuvant Activity of IL-2 Presenting Spores of Bacillus subtilis in a Murine Model of Helicobacter pylori Vaccination
title_fullStr Mucosal Adjuvant Activity of IL-2 Presenting Spores of Bacillus subtilis in a Murine Model of Helicobacter pylori Vaccination
title_full_unstemmed Mucosal Adjuvant Activity of IL-2 Presenting Spores of Bacillus subtilis in a Murine Model of Helicobacter pylori Vaccination
title_short Mucosal Adjuvant Activity of IL-2 Presenting Spores of Bacillus subtilis in a Murine Model of Helicobacter pylori Vaccination
title_sort mucosal adjuvant activity of il-2 presenting spores of bacillus subtilis in a murine model of helicobacter pylori vaccination
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3990602/
https://www.ncbi.nlm.nih.gov/pubmed/24743850
http://dx.doi.org/10.1371/journal.pone.0095187
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AT piatekiwona mucosaladjuvantactivityofil2presentingsporesofbacillussubtilisinamurinemodelofhelicobacterpylorivaccination
AT peszynskasularzgrazyna mucosaladjuvantactivityofil2presentingsporesofbacillussubtilisinamurinemodelofhelicobacterpylorivaccination
AT isticatorachele mucosaladjuvantactivityofil2presentingsporesofbacillussubtilisinamurinemodelofhelicobacterpylorivaccination
AT riccaezio mucosaladjuvantactivityofil2presentingsporesofbacillussubtilisinamurinemodelofhelicobacterpylorivaccination
AT obuchowskimichał mucosaladjuvantactivityofil2presentingsporesofbacillussubtilisinamurinemodelofhelicobacterpylorivaccination
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