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The Relationship between TTF-1 Expression and EGFR Mutations in Lung Adenocarcinomas

OBJECTIVE: To explore the relationship between TTF-1 and EGFR mutations in lung adenocarcinoma tissues to guide clinical treatment timely and effectively. MATERIALS AND METHODS: we collected 664 tissue samples from patients with histologically confirmed lung adenocarcinoma from May 2010 to April 201...

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Autores principales: Shanzhi, Wan, Yiping, Han, Ling, Huang, Jianming, Zheng, Qiang, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3990660/
https://www.ncbi.nlm.nih.gov/pubmed/24743427
http://dx.doi.org/10.1371/journal.pone.0095479
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author Shanzhi, Wan
Yiping, Han
Ling, Huang
Jianming, Zheng
Qiang, Li
author_facet Shanzhi, Wan
Yiping, Han
Ling, Huang
Jianming, Zheng
Qiang, Li
author_sort Shanzhi, Wan
collection PubMed
description OBJECTIVE: To explore the relationship between TTF-1 and EGFR mutations in lung adenocarcinoma tissues to guide clinical treatment timely and effectively. MATERIALS AND METHODS: we collected 664 tissue samples from patients with histologically confirmed lung adenocarcinoma from May 2010 to April 2013. All tumor tissues were collected prior to administering therapy. TTF-1 was detected byimmunohistochemistry and EGFR mutations by DNA direct sequencing. Finally, the correlation between TTF-1 expression and the presence of EGFR mutations was analyzed using χ(2) test or Fisher’s exact test with SPSS software version 18.0. RESULTS: Of the 664 lung adenocarcinoma tissue samples, 18 were partially positive for TTF-1 (+−), and 636 were positive for TTF-1 (+) resulting in a total positive rate of 98.49% (+,+−)(including partial positive). In only 10 cases was the TTF-1 negative (−); the negative rate was 1.51%. There were 402 cases without an EGFR mutation and 262 cases with EGFR mutations; the rate of mutations was 39.46%. The location of the EGFR mutation was exon 19 for 121 cases resulting in a mutation rate in exon 19 of 18.22%. The location of the EGFR mutation was exon 21 for 141 cases resulting in a mutation rate in exon 21 of 21.23%. Exon 18 and 20 detected by DNA direct sequencing no mutations.A Fisher’s exact test was used to determine the correlation between EGFR mutations and TTF-1 expression.for the whole, TTF-1 positive expression(including partial positive) has correlation with EGFR mutations (p<0.001),especially for Exon 21 expression,the correlation is significant (p = 0.008). CONCLUSION: In lung adenocarcinomas, positive and partial positive TTF-1 expression has a significant positive correlation with EGFR mutations(exon 19 and 21). In clinical practice, TTF-1 expression combine with EGFR mutations, especially exon 21 mutation can guide clinical treatment timely for lung adenocarcinomas.
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spelling pubmed-39906602014-04-21 The Relationship between TTF-1 Expression and EGFR Mutations in Lung Adenocarcinomas Shanzhi, Wan Yiping, Han Ling, Huang Jianming, Zheng Qiang, Li PLoS One Research Article OBJECTIVE: To explore the relationship between TTF-1 and EGFR mutations in lung adenocarcinoma tissues to guide clinical treatment timely and effectively. MATERIALS AND METHODS: we collected 664 tissue samples from patients with histologically confirmed lung adenocarcinoma from May 2010 to April 2013. All tumor tissues were collected prior to administering therapy. TTF-1 was detected byimmunohistochemistry and EGFR mutations by DNA direct sequencing. Finally, the correlation between TTF-1 expression and the presence of EGFR mutations was analyzed using χ(2) test or Fisher’s exact test with SPSS software version 18.0. RESULTS: Of the 664 lung adenocarcinoma tissue samples, 18 were partially positive for TTF-1 (+−), and 636 were positive for TTF-1 (+) resulting in a total positive rate of 98.49% (+,+−)(including partial positive). In only 10 cases was the TTF-1 negative (−); the negative rate was 1.51%. There were 402 cases without an EGFR mutation and 262 cases with EGFR mutations; the rate of mutations was 39.46%. The location of the EGFR mutation was exon 19 for 121 cases resulting in a mutation rate in exon 19 of 18.22%. The location of the EGFR mutation was exon 21 for 141 cases resulting in a mutation rate in exon 21 of 21.23%. Exon 18 and 20 detected by DNA direct sequencing no mutations.A Fisher’s exact test was used to determine the correlation between EGFR mutations and TTF-1 expression.for the whole, TTF-1 positive expression(including partial positive) has correlation with EGFR mutations (p<0.001),especially for Exon 21 expression,the correlation is significant (p = 0.008). CONCLUSION: In lung adenocarcinomas, positive and partial positive TTF-1 expression has a significant positive correlation with EGFR mutations(exon 19 and 21). In clinical practice, TTF-1 expression combine with EGFR mutations, especially exon 21 mutation can guide clinical treatment timely for lung adenocarcinomas. Public Library of Science 2014-04-17 /pmc/articles/PMC3990660/ /pubmed/24743427 http://dx.doi.org/10.1371/journal.pone.0095479 Text en © 2014 Shanzhi et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Shanzhi, Wan
Yiping, Han
Ling, Huang
Jianming, Zheng
Qiang, Li
The Relationship between TTF-1 Expression and EGFR Mutations in Lung Adenocarcinomas
title The Relationship between TTF-1 Expression and EGFR Mutations in Lung Adenocarcinomas
title_full The Relationship between TTF-1 Expression and EGFR Mutations in Lung Adenocarcinomas
title_fullStr The Relationship between TTF-1 Expression and EGFR Mutations in Lung Adenocarcinomas
title_full_unstemmed The Relationship between TTF-1 Expression and EGFR Mutations in Lung Adenocarcinomas
title_short The Relationship between TTF-1 Expression and EGFR Mutations in Lung Adenocarcinomas
title_sort relationship between ttf-1 expression and egfr mutations in lung adenocarcinomas
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3990660/
https://www.ncbi.nlm.nih.gov/pubmed/24743427
http://dx.doi.org/10.1371/journal.pone.0095479
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