Complete Genome Sequences of Eight Helicobacter pylori Strains with Different Virulence Factor Genotypes and Methylation Profiles, Isolated from Patients with Diverse Gastrointestinal Diseases on Okinawa Island, Japan, Determined Using PacBio Single-Molecule Real-Time Technology

We report the complete genome sequences of eight Helicobacter pylori strains isolated from patients with gastrointestinal diseases in Okinawa, Japan. Whole-genome sequencing and DNA methylation detection were performed using the PacBio platform. De novo assembly determined a single, complete contig...

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Autores principales: Satou, Kazuhito, Shiroma, Akino, Teruya, Kuniko, Shimoji, Makiko, Nakano, Kazuma, Juan, Ayaka, Tamotsu, Hinako, Terabayashi, Yasunobu, Aoyama, Misako, Teruya, Morimi, Suzuki, Rumiko, Matsuda, Miyuki, Sekine, Akihiro, Kinjo, Nagisa, Kinjo, Fukunori, Yamaoka, Yoshio, Hirano, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2014
Materias:
Prokaryotes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3990747/
https://www.ncbi.nlm.nih.gov/pubmed/24744331
http://dx.doi.org/10.1128/genomeA.00286-14
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author Satou, Kazuhito
Shiroma, Akino
Teruya, Kuniko
Shimoji, Makiko
Nakano, Kazuma
Juan, Ayaka
Tamotsu, Hinako
Terabayashi, Yasunobu
Aoyama, Misako
Teruya, Morimi
Suzuki, Rumiko
Matsuda, Miyuki
Sekine, Akihiro
Kinjo, Nagisa
Kinjo, Fukunori
Yamaoka, Yoshio
Hirano, Takashi
author_facet Satou, Kazuhito
Shiroma, Akino
Teruya, Kuniko
Shimoji, Makiko
Nakano, Kazuma
Juan, Ayaka
Tamotsu, Hinako
Terabayashi, Yasunobu
Aoyama, Misako
Teruya, Morimi
Suzuki, Rumiko
Matsuda, Miyuki
Sekine, Akihiro
Kinjo, Nagisa
Kinjo, Fukunori
Yamaoka, Yoshio
Hirano, Takashi
author_sort Satou, Kazuhito
collection PubMed
description We report the complete genome sequences of eight Helicobacter pylori strains isolated from patients with gastrointestinal diseases in Okinawa, Japan. Whole-genome sequencing and DNA methylation detection were performed using the PacBio platform. De novo assembly determined a single, complete contig for each strain. Furthermore, methylation analysis identified virulence factor genotype-dependent motifs.
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spelling pubmed-39907472014-04-18 Complete Genome Sequences of Eight Helicobacter pylori Strains with Different Virulence Factor Genotypes and Methylation Profiles, Isolated from Patients with Diverse Gastrointestinal Diseases on Okinawa Island, Japan, Determined Using PacBio Single-Molecule Real-Time Technology Satou, Kazuhito Shiroma, Akino Teruya, Kuniko Shimoji, Makiko Nakano, Kazuma Juan, Ayaka Tamotsu, Hinako Terabayashi, Yasunobu Aoyama, Misako Teruya, Morimi Suzuki, Rumiko Matsuda, Miyuki Sekine, Akihiro Kinjo, Nagisa Kinjo, Fukunori Yamaoka, Yoshio Hirano, Takashi Genome Announc Prokaryotes We report the complete genome sequences of eight Helicobacter pylori strains isolated from patients with gastrointestinal diseases in Okinawa, Japan. Whole-genome sequencing and DNA methylation detection were performed using the PacBio platform. De novo assembly determined a single, complete contig for each strain. Furthermore, methylation analysis identified virulence factor genotype-dependent motifs. American Society for Microbiology 2014-04-17 /pmc/articles/PMC3990747/ /pubmed/24744331 http://dx.doi.org/10.1128/genomeA.00286-14 Text en Copyright © 2014 Satou et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license (http://creativecommons.org/licenses/by/3.0/) .
spellingShingle Prokaryotes
Satou, Kazuhito
Shiroma, Akino
Teruya, Kuniko
Shimoji, Makiko
Nakano, Kazuma
Juan, Ayaka
Tamotsu, Hinako
Terabayashi, Yasunobu
Aoyama, Misako
Teruya, Morimi
Suzuki, Rumiko
Matsuda, Miyuki
Sekine, Akihiro
Kinjo, Nagisa
Kinjo, Fukunori
Yamaoka, Yoshio
Hirano, Takashi
Complete Genome Sequences of Eight Helicobacter pylori Strains with Different Virulence Factor Genotypes and Methylation Profiles, Isolated from Patients with Diverse Gastrointestinal Diseases on Okinawa Island, Japan, Determined Using PacBio Single-Molecule Real-Time Technology
title Complete Genome Sequences of Eight Helicobacter pylori Strains with Different Virulence Factor Genotypes and Methylation Profiles, Isolated from Patients with Diverse Gastrointestinal Diseases on Okinawa Island, Japan, Determined Using PacBio Single-Molecule Real-Time Technology
title_full Complete Genome Sequences of Eight Helicobacter pylori Strains with Different Virulence Factor Genotypes and Methylation Profiles, Isolated from Patients with Diverse Gastrointestinal Diseases on Okinawa Island, Japan, Determined Using PacBio Single-Molecule Real-Time Technology
title_fullStr Complete Genome Sequences of Eight Helicobacter pylori Strains with Different Virulence Factor Genotypes and Methylation Profiles, Isolated from Patients with Diverse Gastrointestinal Diseases on Okinawa Island, Japan, Determined Using PacBio Single-Molecule Real-Time Technology
title_full_unstemmed Complete Genome Sequences of Eight Helicobacter pylori Strains with Different Virulence Factor Genotypes and Methylation Profiles, Isolated from Patients with Diverse Gastrointestinal Diseases on Okinawa Island, Japan, Determined Using PacBio Single-Molecule Real-Time Technology
title_short Complete Genome Sequences of Eight Helicobacter pylori Strains with Different Virulence Factor Genotypes and Methylation Profiles, Isolated from Patients with Diverse Gastrointestinal Diseases on Okinawa Island, Japan, Determined Using PacBio Single-Molecule Real-Time Technology
title_sort complete genome sequences of eight helicobacter pylori strains with different virulence factor genotypes and methylation profiles, isolated from patients with diverse gastrointestinal diseases on okinawa island, japan, determined using pacbio single-molecule real-time technology
topic Prokaryotes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3990747/
https://www.ncbi.nlm.nih.gov/pubmed/24744331
http://dx.doi.org/10.1128/genomeA.00286-14
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